Human Cancer Discovery Array (30 targets) (SARB0029)
- SKU:
- SARB0029
- Product Type:
- Protein Array
- Analytes:
- Alpha-fetoprotein
- Beta-2 Microglobulin
- bFGF
- CA125
- CA19-9
- CEA
- CRP (C-Reactive Protein)
- DKK-1
- ErbB2
- Ferritin
- FSH
- Galectin-3
- GDF-15
- HGF
- ICAM-1 (CD54)
- IL-8 (CXCL8)
- MIF
- MMP-2
- NSE
- Osteoprotegerin (TNFRSF11B)
- Osteopontin (SPP1)
- PAI-1
- PSA-free
- RANTES (CCL5)
- SCF
- TGF beta 1
- Thrombospondin 1
- TIMP-1
- uPA
- VEGF-A
- Reactivity:
- Human
- Applications:
- Multiplex Array
Frequently bought together:
Description
system_update_altDatasheet
Product Sku: | SARB0029 |
Size: | 2, 4, or 8 |
Species Detected: | Human |
Number of Targets Detected: | 30 |
Gene Symbols: | AFP, B2M, CA (19-9), CCL5, CEACAM5, CGA, CRP, CXCL8, DKK1, ENO2|ERBB2, FGF2, FSHB, FTH1, GDF15, HGF, ICAM1, KITLG, KLK3, LGALS3, MIF, MMP2, MUC16, PRAP1, SERPINE1, SPP1, TGFB1, THBS1, TIMP1, TNFRSF11B, VEGFA |
Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
Design Principle: | Sandwich-based |
Method of Detection: | Chemiluminescence |
Pathway: | |
Quantitative/Semi-Quantitative: | Semi-Quantitative |
Solid Support: | Membrane |
Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Hover over target for more information | ||||
---|---|---|---|---|
Alpha-fetoprotein
| Beta-2 Microglobulin
| bFGF
| CA125
| CA19-9
|
CEA
| CRP (C-Reactive Protein)
| DKK-1
| ErbB2
| Ferritin
|
FSH
| Galectin-3
| GDF-15
| HGF
| ICAM-1 (CD54)
|
IL-8 (CXCL8)
| MIF
| MMP-2
| NSE
| Osteoprotegerin (TNFRSF11B)
|
Osteopontin (SPP1)
| PAI-1
| PSA-free
| RANTES (CCL5)
| SCF
|
TGF beta 1
| Thrombospondin 1
| TIMP-1
| uPA
| VEGF-A
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cancer Discovery Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1. | Block membranes |
2. | Incubate with Sample |
3. | Incubate with Biotinylated Detection Antibody Cocktail |
4. | Incubate with HRP-Conjugated Streptavidin |
5. | Incubate with Detection Buffers |
6. | Image with chemiluminescent imaging system |
7. | Perform densitometry and analysis |