Description
system_update_altDatasheet
Human Cytokine Array (40 targets) (SARB0033)
The Human Cytokine Array (40 Targets) SARb0033 is a comprehensive research tool for analyzing cytokine levels in human samples. This array features 40 different cytokine targets, allowing for a broad assessment of immune response and inflammation. Designed for use with human samples, this cytokine array is a valuable tool for researchers studying various disease states, including autoimmune disorders, cancer, and inflammatory conditions. By simultaneously measuring multiple cytokines, researchers can gain a deeper understanding of the immune response and identify potential therapeutic targets.
With its high sensitivity and specificity, the Human Cytokine Array SARb0033 provides reliable and accurate results for a wide range of applications, including biomarker discovery, drug development, and disease monitoring. This array offers a convenient and cost-effective solution for studying cytokine profiles and unraveling the complexities of immune regulation.
| Product Sku: | SARB0033 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 40 |
| Gene Symbols: | ADAM9, BMP2, C5, CD200, CD244, CD97, CTSL, FABP2, FAP, FGF19|FRZB, GDF2, GPNMB, IFNAR2, IGF2R, IL1RL2, IL24, IL33, KLK14, LGALS3, LGMN, MBL2, MET, MME, NOTCH1, NOV, OLR1, PDCD1, PGLYRP1, RARRES2, SERPINA4, TF, THBD, TLR2, TNFRSF6B, TNFSF13, WIF1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
2B4 (CD244)
| ADAM-9
| APRIL
| BMP-2
| BMP-9
|
Cathepsin L
| CD200
| CD97
| Chemerin
| C5a
|
DcR3
| FABP2
| FAP
| FGF-19
| Galectin-3
|
HGFR
| IFN-alpha / beta R2
| IGF-2 R
| IL-1 R6 (IL-1 Rrp2)
| IL-24
|
IL-33 (IL-1 F11)
| Kallikrein 14
| Legumain
| LOX-1
| MBL
|
Neprilysin
| Notch-1
| NOV (CCN3)
| Osteoactivin (GPNMB)
| PD-1
|
PGRPs
| SERPINA4
| sFRP-3
| Thrombomodulin
| TLR2
|
Transferrin
| WIF-1
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C11 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
