Description
Human NF-kB Pathway Phosphorylation Array (11 targets) (SARB0059)
The Human NF-kB Pathway Phosphorylation Array (SARB0059) offered by AssayGenie is a powerful tool for studying the activation of the NF-kB signaling pathway. This array contains antibodies specific to 11 target proteins involved in the NF-kB pathway, allowing for the simultaneous detection and analysis of phosphorylation events.The NF-kB pathway is crucial for regulating immune responses, inflammation, and cell survival, making it a key focus in research related to cancer, autoimmune diseases, and inflammatory conditions. By using the Human NF-kB Pathway Phosphorylation Array, researchers can gain insights into the activation status of key proteins in this pathway, facilitating the identification of potential drug targets and biomarkers for disease diagnosis and prognosis.
This array is compatible with various sample types, including cell lysates and tissue homogenates, and its high sensitivity and specificity ensure reliable and reproducible results. Whether studying basic mechanisms of cell signaling or exploring potential therapeutic interventions, the Human NF-kB Pathway Phosphorylation Array is a valuable resource for researchers in immunology, oncology, and beyond.
| Product Sku: | SARB0059 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 11 |
| Gene Symbols: | ATM, EIF2S1, HDAC2, HDAC4, MAP3K7, NFKBIA, RELA, RPS6KA5, STAT1, TBK1|ZAP70 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | MAPK Signaling / mTOR Signaling / NFkB Signaling / p53 Signaling / PKC Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
ATM (P-Ser1981)
| eIF-2a (P-Ser52)
| HDAC2 (P-Ser394)
| HDAC4 (P-Ser632)
| IKB-alpha (P-Ser32)
|
MSK1 (P-Ser376)
| NF-kB (P-Ser536)
| STAT1 (P-Ser727)
| TAK1 (P-Ser412)
| TBK1 (P-Ser172)
|
ZAP70 (P-Tyr292)
| ||||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human NF-kB Pathway Phosphorylation Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit-IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or plastic Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system: CCD camera, X-ray Film and a suitable film processor, gel documentation system, or another chemiluminescent detection system capable of imaging a western blot.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated anti-IgG |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
