Description
Human RB1 Recombinant Protein (N-GST)
Human RB1 Recombinant Protein (N-GST) is a recombinant protein expressed in E. coli, spanning Gln639-Thr778, with an N-terminal GST tag. Predicted molecular weight: 43.67 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Retinoblastoma-associated protein (RB1) is a ~106 kDa protein. Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle. For research use only.
The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes. Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription. Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase. RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C.
| Product Name: | Human RB1 Recombinant Protein (N-GST) |
| SKU: | GXRP00625 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | pRb, p110-RB1, Retinoblastoma-associated protein, Rb, p105-Rb, RB1, pp110 |
| Tag: | N-GST |
| Expression Host: | E. coli |
| Protein Length: | Gln639-Thr778 |
| Calculated MW: | 43.67 kDa |
| UniProt: | P06400 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
