Anti-FSCN1 Antibody (CAB1904)

Antibody Name:	Anti-FSCN1 Antibody [KO Validated]
Antibody SKU:	CAB1904
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human FSCN1 (NP_003079.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, HeLa, NCI-H460, HepG2, SH-SY5Y, Mouse brain, Mouse spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human FSCN1 (NP_003079.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FIGC RKVT GTLD ANRS SYDV FQLE FNDG AYNI KDST GKYW TVGS DSAV TSSG DTPV DFFF EFCD YNKV AIKV GGRY LKGD HAGV LKAS AETV DPAS LWEY
Gene ID:	6624
Uniprot:	Q16658
Cellular Location:	Cell junction, Cell projection, Cytoplasm, cytoskeleton, cytosol, filopodium, invadopodium, microvillus
Calculated MW:	54kDa
Observed MW:	50kDa, 55kDa

Synonyms:

FSCN1, FAN1, HSN, SNL, p55, fascin

Background:

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15.

UniProt Protein Function:	Fascin: Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Associates with beta-catenin. Ubiquitous. Belongs to the fascin family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding Chromosomal Location of Human Ortholog: 7p22 Cellular Component: cytoplasm; stress fiber; cell junction; cytosol; actin cytoskeleton; filopodium Molecular Function:protein binding, bridging; actin filament binding; protein binding; drug binding; actin binding Biological Process: cell proliferation; actin filament bundle formation; cell migration; cell motility involved in cell locomotion; actin cytoskeleton organization and biogenesis
NCBI Summary:	This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]
UniProt Code:	Q16658
NCBI GenInfo Identifier:	2498357
NCBI Gene ID:	6624
NCBI Accession:	Q16658.3
UniProt Related Accession:	Q16658
Molecular Weight:	54kDa
NCBI Full Name:	Fascin
NCBI Synonym Full Names:	fascin actin-bundling protein 1
NCBI Official Symbol:	FSCN1
NCBI Official Synonym Symbols:	HSN; SNL; p55; FAN1
NCBI Protein Information:	fascin
UniProt Protein Name:	Fascin
UniProt Synonym Protein Names:	55 kDa actin-bundling protein; Singed-like protein; p55
Protein Family:	Fascin
UniProt Gene Name:	FSCN1
UniProt Entry Name:	FSCN1_HUMAN

	Western blot analysis of extracts of various cell lines, using FSCN1 antibody (CAB1904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts from normal (control) and FSCN1 knockout (KO) HeLa cells, using FSCN1 antibody (CAB1904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded mouse testis using [KO Validated] FSCN1 Rabbit pAb (CAB1904) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using [KO Validated] FSCN1 Rabbit pAb (CAB1904) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] FSCN1 Rabbit pAb (CAB1904) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-FSCN1 Antibody (CAB13355)	Human Fascin ELISA Kit
Anti-Fascin Antibody (CAB9566)	Human FSCN (Fascin) CLIA Kit (HUES00809)
FSCN1 Antibody (PACO01925)	Human Fascin (FSCN1) ELISA Kit
FSCN1 Antibody (PACO09350)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)