Mouse Cytokine Array (144 targets)

Product Sku:	SARB0079
Size:	2, 4, or 8
Species Detected:	Mouse
Number of Targets Detected:	144
Gene Symbols:	ACE, AGER, AREG, AXL, CCL1, CCL11, CCL12, CCL17, CCL19, CCL2\|CCL20, CCL21, CCL22, CCL24, CCL25, CCL27, CCL3, CCL5, CCL9, CD27, CD36, CD40, CD40LG, CD70, CDH1, CHRD, CSF1, CSF2, CSF2RB, CSF3, CTF1, CTLA4, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL15, CXCL16, CXCL2, CXCL5, CXCL9, DCN, DKK1, DPP4, EGF, ENG, EPGN, EREG, F11R, FASLG, FCGR2B, FGF2, FIGF, FLT1, FLT3LG, FLT4, GAS1, GAS6, GZMB, HGF, ICAM1, IFNG, IFNL2, IGF1, IGF2, IGFBP2, IGFBP3, IGFBP5, IGFBP6, IL10, IL11, IL12A, IL13, IL15, IL17A, IL17B, IL17F, IL17RB, IL1A, IL1B, IL1RL1, IL1RN, IL2, IL20, IL21, IL25, IL2RA, IL3, IL4, IL5, IL6, IL6R, IL7, IL9, KDR, KITLG, LEP, LEPR, LGALS1, MADCAM1, MET, MFGE8, MME, MMP2, MMP3, MMP9, PF4, PPBP, PRL, PTX3, RETN, SELE, SELL, SELP, SHH, SLPI, SPINT1, SPP1, THPO, TIMP1, TIMP2, TNF, TNFRSF11B, TNFRSF13B, TNFRSF18, TNFRSF19, TNFRSF8, TNFRSF9, TNFSF11, TNFSF12, TNFSF18, TNFSF8, TNFSF9, TPO, TREM1, TSLP, TYRO3, VCAM1, VEGFA, XCL1
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
4-1BB (TNFRSF9/CD137) Gene Symbol: TNFRSF9 Gene ID: N/A Accession #: P20334	6Ckine (CCL21) Gene Symbol: CCL21 Gene ID: N/A Accession #: P86792	ACE Gene Symbol: ACE Gene ID: N/A Accession #: P22967	ALK-1 Gene Symbol: SLPI Gene ID: N/A Accession #: O09081	Amphiregulin Gene Symbol: AREG Gene ID: N/A Accession #: P31955
Axl Gene Symbol: Axl Gene ID: N/A Accession #: Q00993	bFGF Gene Symbol: FGF2 Gene ID: N/A Accession #: P15655	BLC (CXCL13) Gene Symbol: CXCL13 Gene ID: N/A Accession #: O55038	Cardiotrophin-1 (CT-1) Gene Symbol: CTF1 Gene ID: N/A Accession #: Q60753	CD27 (TNFRSF7) Gene Symbol: CD27 Gene ID: N/A Accession #: P41272
CD27 Ligand (TNFSF7) Gene Symbol: CD70 Gene ID: N/A Accession #: P32970	CD30 Ligand (TNFSF8) Gene Symbol: CD30LG Gene ID: N/A Accession #: P32972	CD30 (TNFRSF8) Gene Symbol: CD30 Gene ID: N/A Accession #: Q60846	CD36 (SR-B3) Gene Symbol: CD36 Gene ID: N/A Accession #: Q08857	CD40 (TNFRSF5) Gene Symbol: CD40 Gene ID: N/A Accession #: Q99NE2
CD40 Ligand (TNFSF5) Gene Symbol: CD40LG Gene ID: N/A Accession #: P27548	Chordin Gene Symbol: CHRD Gene ID: N/A Accession #: Q9Z0E2	CRG-2 Gene Symbol: Cxcl10 Gene ID: N/A Accession #: P17515	CTACK (CCL27) Gene Symbol: CCL27 Gene ID: N/A Accession #: Q9Z1X0	CTLA-4 (CD152) Gene Symbol: Ctla4 Gene ID: N/A Accession #: Q9QZZ7
CXCL16 Gene Symbol: CXCL16 Gene ID: N/A Accession #: Q5F2D5	Decorin Gene Symbol: DCN Gene ID: N/A Accession #: P28654	DKK-1 Gene Symbol: DKK1 Gene ID: N/A Accession #: Q80UL5	CD26 (DPPIV) Gene Symbol: DPP4 Gene ID: N/A Accession #: P28843	Dtk Gene Symbol: TYRO3 Gene ID: N/A Accession #: O09080
E-Cadherin Gene Symbol: CDH1 Gene ID: N/A Accession #: P09803	EGF Gene Symbol: EGF Gene ID: N/A Accession #: E9QNX6	Endoglin (CD105) Gene Symbol: ENG Gene ID: N/A Accession #: Q63961	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Gene ID: N/A Accession #: P48298	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Gene ID: N/A Accession #: Q9JKC0
Epigen Gene Symbol: EPGN Gene ID: N/A Accession #: Q8CEX5	Epiregulin Gene Symbol: EREG Gene ID: N/A Accession #: Q61521	E-Selectin Gene Symbol: SELE Gene ID: N/A Accession #: Q00690	Fas Ligand (TNFSF6) Gene Symbol: FASLG Gene ID: N/A Accession #: P41047	Fc gamma RIIB (CD32b) Gene Symbol: FCGR2B Gene ID: N/A Accession #: P08101
Flt-3 Ligand Gene Symbol: FLT3LG Gene ID: N/A Accession #: P49772	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Gene ID: N/A Accession #: O35188	Galectin-1 Gene Symbol: LGALS1 Gene ID: N/A Accession #: P17601	GCSF Gene Symbol: CSF3 Gene ID: N/A Accession #: P09920	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Gene ID: N/A Accession #: Q9JKR2
GITR Ligand (TNFSF18) Gene Symbol: TNFSF18 Gene ID: N/A Accession #: Q7TS55	GM-CSF Gene Symbol: CSF2 Gene ID: N/A Accession #: P26955	Granzyme B Gene Symbol: GZMB Gene ID: N/A Accession #: Q3V2P1	Gas 1 Gene Symbol: GAS1 Gene ID: N/A Accession #: Q01721	Gas 6 Gene Symbol: Gas6 Gene ID: N/A Accession #: Q99K57
HAI-1 Gene Symbol: SPINT1 Gene ID: N/A Accession #: Q9R097	HGF Gene Symbol: HGF Gene ID: N/A Accession #: Q53WS5	HGFR Gene Symbol: MET Gene ID: N/A Accession #: D3YVY2	ICAM-1 (CD54) Gene Symbol: ICAM1 Gene ID: N/A Accession #: P13597	IFN-gamma Gene Symbol: IFNG Gene ID: N/A Accession #: P01580
IGFBP-2 Gene Symbol: IGFBP2 Gene ID: N/A Accession #: P47877	IGFBP-3 Gene Symbol: IGFBP3 Gene ID: N/A Accession #: P47878	IGFBP-5 Gene Symbol: IGFBP5 Gene ID: N/A Accession #: Q07079	IGFBP-6 Gene Symbol: IGFBP6 Gene ID: N/A Accession #: Q91X24	IGF-1 Gene Symbol: IGF1 Gene ID: N/A Accession #: P05018
IGF-2 Gene Symbol: Igf2 Gene ID: N/A Accession #: P09535	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: N/A Accession #: P10749	IL-1 R4 (ST2) Gene Symbol: IL1RL1 Gene ID: N/A Accession #: Q05208	IL-10 Gene Symbol: IL10 Gene ID: N/A Accession #: Q0VBJ1	IL-11 Gene Symbol: IL11 Gene ID: N/A Accession #: P47873
IL-12 p40/p70 Gene Symbol: IL12A; IL12B Gene ID: N/A Accession #: P43432	IL-12 p70 Gene Symbol: IL12A Gene ID: N/A Accession #: P43431	IL-13 Gene Symbol: IL13 Gene ID: N/A Accession #: P20109	IL-15 Gene Symbol: IL15 Gene ID: N/A Accession #: Q1AHQ7	IL-17A Gene Symbol: IL17A Gene ID: N/A Accession #: Q60971
IL-17B Gene Symbol: IL17B Gene ID: N/A Accession #: Q99MY3	IL-17 RB Gene Symbol: IL17RB Gene ID: N/A Accession #: Q9JIP3	IL-17E (IL-25) Gene Symbol: IL25 Gene ID: N/A Accession #: Q8VHH8	IL-17F Gene Symbol: IL17F Gene ID: N/A Accession #: Q7TNI7	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: N/A Accession #: P01582
IL-1 RA (IL-1 F3) Gene Symbol: IL1RN Gene ID: N/A Accession #: P25085	IL-2 Gene Symbol: IL2 Gene ID: N/A Accession #: Q791T3	IL-2 R alpha Gene Symbol: IL2RA Gene ID: N/A Accession #: Q61731	IL-20 Gene Symbol: IL20 Gene ID: N/A Accession #: Q9JKV9	IL-21 Gene Symbol: IL21 Gene ID: N/A Accession #: E9PX58
IL-28A (IFN-lambda 2) Gene Symbol: IFNL2 Gene ID: N/A Accession #: Q4VK74	IL-3 Gene Symbol: IL3 Gene ID: N/A Accession #: P01586	IL-3 R beta Gene Symbol: Csf2rb Gene ID: N/A Accession #: P26955	IL-4 Gene Symbol: IL4 Gene ID: N/A Accession #: P07750	IL-5 Gene Symbol: IL5 Gene ID: N/A Accession #: P04401
IL-6 Gene Symbol: IL6 Gene ID: N/A Accession #: P08505	IL-6 R Gene Symbol: IL6R Gene ID: N/A Accession #: P22272	IL-7 Gene Symbol: IL7 Gene ID: N/A Accession #: P10168	IL-9 Gene Symbol: IL9 Gene ID: N/A Accession #: P15247	I-TAC (CXCL11) Gene Symbol: CXCL11 Gene ID: N/A Accession #: Q9JHH5
JAM-A (CD321/F11R) Gene Symbol: F11R Gene ID: N/A Accession #: Q8VC39	KC (CXCL1) Gene Symbol: CXCL1 Gene ID: N/A Accession #: P12850	Leptin Gene Symbol: LEP Gene ID: N/A Accession #: P41160	Leptin R Gene Symbol: LEPR Gene ID: N/A Accession #: Q61215	LIX Gene Symbol: Cxcl5 Gene ID: N/A Accession #: P50228
L-Selectin (CD62L) Gene Symbol: SELL Gene ID: N/A Accession #: Q3TCF3	Lungkine (CXCL15) Gene Symbol: CXCL15 Gene ID: N/A Accession #: Q3UQ15	Lymphotactin (XCL1) Gene Symbol: XCL1 Gene ID: N/A Accession #: P47993	MAdCAM-1 Gene Symbol: MADCAM1 Gene ID: N/A Accession #: Q61278	MCP-1 (CCL2) Gene Symbol: CCL2 Gene ID: N/A Accession #: Q9QYD7
MCP-5 Gene Symbol: Ccl12 Gene ID: N/A Accession #: Q9QYD6	M-CSF Gene Symbol: CSF1 Gene ID: N/A Accession #: P07141	MDC (CCL22) Gene Symbol: CCL22 Gene ID: N/A Accession #: O88430	MFG-E8 Gene Symbol: Mfge8 Gene ID: N/A Accession #: Q9R1X9	MIG (CXCL9) Gene Symbol: CXCL9 Gene ID: N/A Accession #: P18340
MIP-1 alpha (CCL3) Gene Symbol: CCL3 Gene ID: N/A Accession #: P14096	MIP-1 gamma Gene Symbol: Ccl9 Gene ID: N/A Accession #: Q5QNW2	MIP-2 Gene Symbol: Cxcl2 Gene ID: N/A Accession #: P10889	MIP-3 beta (CCL19) Gene Symbol: CCL19 Gene ID: N/A Accession #: O70460	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Gene ID: N/A Accession #: O89093
MMP-2 Gene Symbol: MMP2 Gene ID: N/A Accession #: P33434	MMP-3 Gene Symbol: MMP3 Gene ID: N/A Accession #: P28862	Neprilysin Gene Symbol: MME Gene ID: N/A Accession #: Q6NXX5	Osteopontin (SPP1) Gene Symbol: SPP1 Gene ID: N/A Accession #: P19008	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Gene ID: N/A Accession #: O70202
Pentraxin-3 (TSG-14) Gene Symbol: PTX3 Gene ID: N/A Accession #: P48759	Platelet Factor 4 (CXCL4) Gene Symbol: PF4, CXCL4, SCYB4 Gene ID: N/A Accession #: Q9Z126	Prolactin Gene Symbol: PRL Gene ID: N/A Accession #: P06879	Pro-MMP-9 Gene Symbol: Mmp9 Gene ID: N/A Accession #: Q80XI8	P-Selectin Gene Symbol: SELP Gene ID: N/A Accession #: Q01102
RAGE Gene Symbol: MOK Gene ID: N/A Accession #: Q9WVS4	RANTES (CCL5) Gene Symbol: CCL5 Gene ID: N/A Accession #: P30882	Resistin Gene Symbol: RETN Gene ID: N/A Accession #: Q99P87	SCF Gene Symbol: KITLG Gene ID: N/A Accession #: P20826	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Gene ID: N/A Accession #: P40224
Sonic Hedgehog N-Terminal (Shh-N) Gene Symbol: SHH Gene ID: N/A Accession #: N/A	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: N/A Accession #: P25118	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: N/A Accession #: O88734	TACI (TNFRSF13B) Gene Symbol: TNFRSF13B Gene ID: N/A Accession #: Q9DBZ3	TARC (CCL17) Gene Symbol: CCL17 Gene ID: N/A Accession #: Q9WUZ6
I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: N/A Accession #: P14098	TECK (CCL25) Gene Symbol: CCL25 Gene ID: N/A Accession #: O35903	TCK-1 (CXCL7) Gene Symbol: Ppbp Gene ID: N/A Accession #: Q9EQI5	TIMP-1 Gene Symbol: TIMP1 Gene ID: N/A Accession #: P12032	TIMP-2 Gene Symbol: TIMP2 Gene ID: N/A Accession #: Q6GXA8
TNF alpha Gene Symbol: TNF Gene ID: N/A Accession #: Q62326	Thrombopoietin (TPO) Gene Symbol: THPO Gene ID: N/A Accession #: P40226	TRANCE (TNFSF11) Gene Symbol: TNFSF11 Gene ID: N/A Accession #: Q9R1Y0	TREM-1 Gene Symbol: TREM1 Gene ID: N/A Accession #: Q3TAL7	TROY (TNFRSF19) Gene Symbol: TNFRSF19 Gene ID: N/A Accession #: Q80T13
TSLP Gene Symbol: TSLP Gene ID: N/A Accession #: Q9JIE6	TWEAK (TNFSF12) Gene Symbol: TNFSF12 Gene ID: N/A Accession #: O54907	TWEAK R (TNFRSF12) Gene Symbol: TNFRSF12A Gene ID: N/A Accession #: Q9CR75	VCAM-1 (CD106) Gene Symbol: VCAM1 Gene ID: N/A Accession #: P29533	VEGF-A Gene Symbol: VEGFA Gene ID: N/A Accession #: Q00731
VEGFR1 Gene Symbol: Flt1 Gene ID: N/A Accession #: Q61517	VEGFR2 Gene Symbol: KDR Gene ID: N/A Accession #: Q3UQZ6	VEGFR3 Gene Symbol: FLT4 Gene ID: N/A Accession #: P35917	VEGF-D Gene Symbol: FIGF Gene ID: N/A Accession #: A2AIH3

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Mouse Cytokine Antibody Array C2000 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis