The Phospho-TIFA-T9 Polyclonal Antibody (CABP1395) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes an adapter protein involved in adaptive and innate immunity. This protein includes a forkhead-associated (FHA) domain that specifically binds to phosphorylated serine and threonine residues. In response to bacterial infection, the encoded host cell protein undergoes an intermolecular interaction between the FHA domain and a phosphorylated threonine that leads to protein oligomerization and stimulation of the NF-kappa B and other downstream signaling pathways. This protein exhibits reduced expression in hepatocellular carcinoma and may suppress hepatocellular carcinoma progression. This protein may also play a role in the DNA damage response. RRID Gene ID 92610 Swiss Prot Synonym T2BP; T6BP; TIFAA; Phospho-TIFA-T9
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-TIFA-T9 Polyclonal Antibody
SKU:
CABP1395
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
T2BP, T6BP, TIFAA, Phospho-TIFA-T9
Positive Sample:
TIFA knockout (KO) 293T treated with ADP-heptose after transfected with human TIFA
Cellular Localization:
Cytoplasm.
Calculated MW:
21 kDa
Observed MW:
21 kDa
This gene encodes an adapter protein involved in adaptive and innate immunity. This protein includes a forkhead-associated (FHA) domain that specifically binds to phosphorylated serine and threonine residues. In response to bacterial infection, the encoded host cell protein undergoes an intermolecular interaction between the FHA domain and a phosphorylated threonine that leads to protein oligomerization and stimulation of the NF-kappa B and other downstream signaling pathways. This protein exhibits reduced expression in hepatocellular carcinoma and may suppress hepatocellular carcinoma progression. This protein may also play a role in the DNA damage response. RRID Gene ID 92610 Swiss Prot Synonym T2BP; T6BP; TIFAA; Phospho-TIFA-T9
Purification Method:
Affinity purification
Gene ID:
92610
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from TIFA knockout (KO) 293T cells transfected with TIFA using Phospho-TIFA-T9 Rabbit pAb (CABP1395) at 1:1000 dilution incubated overnight at 4℃. TIFA knockout (KO) 293T cells transfected with TIFA were treated with ADP-heptose. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
