Description
system_update_altDatasheet
Porcine Cytokine Array (48 targets) (SARB0095)
The Porcine Cytokine Array (SARB0095) from Assay Genie is a powerful tool for researchers studying cytokine expression in porcine samples. This array allows for the simultaneous detection of 48 different cytokines, providing a comprehensive overview of the immune response in porcine models.With a high sensitivity and specificity, this array delivers reliable and reproducible results, making it ideal for a wide range of applications including immunology research, drug development, and biomarker discovery.
The array is easy to use and offers a quick and cost-effective way to analyze cytokine profiles in porcine samples.Whether you are investigating immune responses in infectious diseases, inflammatory conditions, or vaccine development, the Porcine Cytokine Array from Assay Genie is a valuable tool that can help advance your research goals. Order yours today and unlock the potential of cytokine analysis in porcine studies.
| Product Sku: | SARB0095 |
| Size: | 2, 4, or 8 |
| Species Detected: | Porcine |
| Number of Targets Detected: | 48 |
| Gene Symbols: | ANGPT1, CCL4, CCL5, CSF2, CXCL8, CXCL9, DCN, EPO, FGF21, IFN-BETA|IFNB, IFNB1, IFNG, IGF2, IGFBP5, IGIF, IL10, IL12A, IL13, IL15, IL17, IL17A, IL17F, IL18, IL1A, IL1B, IL1RN, IL21, IL22, IL28B, IL4, IL6, IL8, INS, INTERFERON-BETA-1, INTERFERON-BETA/POIFN-BETA, IRAP1, KITLG, MGF, MIF, MIP1B, NCAM1, PDGFB, PECAM1, PLGF, SCY4A, SCYA5, TGFB1, TIMP-2, TIMP1, TIMP2, TNF, TNFA, TNFRSF11B, TNFRSF12A, TNFSF2, VEGF, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Angiopoietin-1
| CCL3L1
| Decorin
| Eotaxin-1 (CCL11)
| Erythropoietin
|
FGF-21
| Galectin-9
| GASP-1 (WFIKKNRP)
| GM-CSF
| IFN alpha
|
IFN-beta
| IFN-gamma
| IGF-2
| IGFBP-5
| IL-1 alpha (IL-1 F1)
|
IL-1 beta (IL-1 F2)
| IL-1 Ra (IL-1 F3)
| IL-10
| IL-12 p70
| IL-13
|
IL-15
| IL-17A
| IL-17F
| IL-18
| IL-21
|
IL-22
| IL-28B
| IL-4
| IL-6
| IL-8 (CXCL8)
|
Insulin
| MIF
| MIG (CXCL9)
| MIP-1 beta (CCL4)
| NCAM-1 (CD56)
|
Osteoprotegerin (TNFRSF11B)
| PDGF-BB
| PECAM-1 (CD31)
| PLGF-2
| RANTES (CCL5)
|
SCF
| TGF alpha
| TGF beta 1
| TIMP-1
| TIMP-2
|
TNF alpha
| TWEAK R (TNFRSF12)
| VEGF-A
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Porcine Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
