The Recombinant Keratin 6 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111205) against Keratin 6. The term 'keratin' covers all intermediate filament-forming proteins with specific physicochemical properties and produced in any vertebrate epithelia. Similarly, the nomenclature of epithelia as cornified, keratinized or non-keratinized is based historically on the notion that only the epidermis of skin modifications such as horns, claws and hooves is cornified. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC and IF applications. It has been validated by WB analysis of A431, Rat skin, Mouse skin, IHC analysis of Human breast, Human esophagus, Human lung squamous carcinoma, Human prostate hyperplasia and IF analysis of A431. The target is localized to the cytoplasm.
Product Name:
Recombinant Keratin 6 Monoclonal Antibody
SKU:
AGEL6510
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
KRT6C, KRT6E
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant human Keratin 6 fragment
Applications:
WBIHCIF
Clonality:
Recombinant Monoclonal
Clone No:
C111205
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Keratin 6
Conjugation:
Unconjugated
Observed MW:
56 kDa
Calculated MW:
60 kDa
Recommended Dilution:
WB 1:500-2000; IHC 1:200-1000; IF 1:50
UniProt:
P48668
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescent analysis of (100% Ice-cold methanol) fixed A431 cells using anti-Keratin 6 Monoclonal Antibody at dilution of 1:50.
Immunohistochemistry of paraffin-embedded Human breast using Keratin 6 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human esophagus using Keratin 6 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human lung squamous carcinoma using Keratin 6 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human prostate hyperplasia using Keratin 6 Monoclonal Antibody at dilution of 1:1000.
Western Blot with Keratin 6 Monoclonal Antibody at dilution of 1:2000. Lane 1: A431, Lane 2: Rat skin, Lane 3: Mouse skin Observed-MW:56 kDa Calculated-MW:60 kDa
