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Converting RPM to G-force in Centrifugation(Post)
Lab Methods · CentrifugationRPM to G-Force (RCF) Conversion: Formula, Calculator Method and Common ErrorsA protocol that says “spin at 3,000 rpm” is incomplete, because the force a sample experiences
Cgas sting pathway deep · HT(Page) Mononuclear Phagocytes in Health and Disease(Post)Mononuclear phagocytes, essential in immunity and homeostasis, perform diverse roles from phagocytosis to tissue repair, influencing health and disease. Key Takeaways: Mononuclear p
CD47: Overcoming Tumor Evasion Through Macrophage Activation(Post)One of the greatest challenges in cancer therapy is how tumors evade immune detection. Among the mechanisms cancer cells exploit, the CD47 pathway has emerged as a major player in helping
Keep your Lab Book up to Date(Post)When PhDs and Post-Docs start in new lab they have the best intentions to keep their lab books up to date with notes, new research methods, data, and protocol information. These best efforts us
Rovalpituzumab Tesirine: Advancing Small Cell Lung Cancer Research(Post)Quick Facts About Rovalpituzumab TesirineWhat is Rovalpituzumab Tesirine?Rovalpituzumab Tesirine (Rova-T) is an antibody-drug conjugate (ADC) designed to target DLL3, a protein overexpre
Spatial Transcriptomics: Mapping the Future of Medicine at Cellular Resolution(Post)Spatial Transcriptomics: Mapping the Future of Medicine at Cellular Resolution Imagine being able to create a detailed map of a city, not just showing the streets and buildings, but also revealing th
Kreuzreaktivität ELISA: Praxisleitfaden für Forschende(Post)Kreuzreaktivität ELISA: Praxisleitfaden für Forschende Kreuzreaktivität im ELISA bedeutet, dass ein Antikörper an ein strukturell ähnliches Nicht-Ziel bindet und dadurch falsch-positive oder verfälsc
Non-Small Cell Lung Carcinoma: Understanding, Diagnosing, and Treating NSCLC(Post)Non-Small Cell Lung Carcinoma (NSCLC) is the most common type of lung cancer, representing about 85% of all lung cancer cases. NSCLC includes several subtypes, with distinct biological ch
Apoptosis Pathway: Death Receptor, Mitochondrial and Granzyme Routes(Post)Apoptosis is not one pathway but three routes into the same execution machinery. A death ligand at the surface, a mitochondrion that loses outer-membrane integrity, and a granzyme delivered by a cyto
Gut Inflammation and Inflammatory Diseases (Post)What is Gut Inflammation? The gut, or gastrointestinal (GI) tract is a long tube that starts from the mouth and extends to the anus. It includes various organs such
Esophageal Cancer: Causes, Symptoms, Diagnosis, Treatment, and Prevention(Post)Esophageal cancer is a type of cancer that affects the esophagus, which is the tube that carries food and liquids from your mouth to your stomach. Esophageal cancer can cause many di
NLRP3 Inflammasome and Pyroptosis: Pathway, Function and Assays(Post)The inflammasome is one of the few innate pathways that destroys the cell it activates. A macrophage that assembles NLRP3 has committed to a decision it cannot reverse: a priming signal through TLR4
Codrituzumab: Unveiling Its Role in Cancer Research and Treatment(Post)What You Need to Know About CodrituzumabWhat is Codrituzumab?Codrituzumab is an investigational monoclonal antibody targeting CD47, an immune checkpoint that regulates immune responses i
Colorectal Cancer Review (CRC) - Assay Genie(Post)By Charlotte O’Donnell PhD Cancer Carcinogenesis is a multi-step process that develops through epigenetic changes and muta
OX40 Agonists: Revitalizing the Immune Response in Cancer(Post)Cancer immunotherapy has revolutionized treatment options, offering strategies to harness the immune system to target and destroy tumors. One emerging area of interest is the use of OX40
ELISA Inter-Plate Variability: Same Sample, Different Results(Post)Quick answer On the Dog Beta-endorphin (BEP) ELISA Kit, a researcher ran the same canine serum aliquots on two plates from the same lot and saw roughly double the concentration on one
Mycoplasma Detection Kit Troubleshooting: The Importance of Cell Confluence(Post)Quick answer A researcher's samples read negative at the standard 1-hour incubation on the MycoGenie Rapid Mycoplasma Detection Kit (MORV0011), but many turned positive after 2 hours.
The role of IL-18 in health and disease(Post)Exploring IL-18: A key cytokine in inflammation and disease. Key Takeaways: IL-18, a pro-inflammatory cytokine, plays a pivotal role in immune responses, particularly in inducing Type II
Six étapes pour un protocole ELISA sandwich reproductible en laboratoire(Post)Le protocole ELISA sandwich standard suit six étapes: revêtement du puits avec l’anticorps de capture, blocage, incubation des standards et échantillons, ajout de l’anticorps de détection, puis du