TFE3 Transcription Factor Activity Assay
- SKU:
- TFAB00180
- Product Type:
- ELISA Kit
- ELISA Type:
- Transcription Factor Assay
- Analytes:
- TFE3
- Reactivity:
- Human
- Mouse
Description
| Product Name: | TFE3 Transcription Factor Activity Assay |
| Product Code: | TFAB00180 |
| Target: | TFE3 |
| Synonyms: | Class E basic helix-loop-helix protein 33, bHLHe33 |
| Reactivity: | Human, Mouse |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie TFE3 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | TFE3: Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein. Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3. Belongs to the MiT/TFE family. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding; Oncoprotein Chromosomal Location of Human Ortholog: Xp11.22 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:protein dimerization activity; transcription factor activity Biological Process: positive regulation of cell adhesion; regulation of osteoclast differentiation; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; humoral immune response Disease: Renal Cell Carcinoma, Xp11-associated |
| NCBI Summary: | This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| UniProt Code: | P19532 |
| NCBI GenInfo Identifier: | 160113240 |
| NCBI Gene ID: | 7030 |
| NCBI Accession: | P19532.4 |
| UniProt Secondary Accession: | P19532,Q5JU74, Q92757, Q92758, Q99964, A8MZL6, |
| UniProt Related Accession: | P19532 |
| Molecular Weight: | 575 |
| NCBI Full Name: | Transcription factor E3 |
| NCBI Synonym Full Names: | transcription factor binding to IGHM enhancer 3 |
| NCBI Official Symbol: | TFE3Â Â |
| NCBI Official Synonym Symbols: | TFEA; RCCP2; RCCX1; bHLHe33Â Â |
| NCBI Protein Information: | transcription factor E3; transcription factor for IgH enhancer; transcription factor E family, member A; class E basic helix-loop-helix protein 33; transcription factor for immunoglobulin heavy-chain enhancer 3 |
| UniProt Protein Name: | Transcription factor E3 |
| UniProt Synonym Protein Names: | Class E basic helix-loop-helix protein 33; bHLHe33 |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | TFE3Â Â |
| UniProt Entry Name: | TFE3_HUMAN |
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