Anti-PPP2CA Antibody (CAB6702)

Antibody Name:	Anti-PPP2CA Antibody
Antibody SKU:	CAB6702
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human PPP2CA (NP_002706.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, MCF7, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human PPP2CA (NP_002706.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDEK VFTK ELDQ WIEQ LNEC KQLS ESQV KSLC EKAK EILT KESN VQEV RCPV TVCG DVHG QFHD LMEL FRIG GKSP DTNY LFMG DYVD RGYY SVET VTLL VALK VRYR ERIT ILRG NHES RQIT QVYG FYDE CLRK YGNA NVWK YFTD LFDY LPLT ALVD GQIF CLHG GLSP SIDT LDHI RALD RLQE VPHE GPMC DLLW SDPD DRGG WGIS PRGA GYTF GQDI SETF NHAN GLTL VSRA HQLV MEGY NWCH DRNV VTIF SAPN YCYR CGNQ AAIM ELDD TLKY SFLQ FDPA PRRG EPHV TRRT PDYF L
Gene ID:	5515
Uniprot:	P67775
Cellular Location:	Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, spindle pole
Calculated MW:	29kDa/35kDa
Observed MW:	36KDa

Synonyms:

PPP2CA, PP2Ac, PP2CA, PP2Calpha, RP-C

Background:

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.

UniProt Protein Function:	PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
UniProt Protein Details:	Protein type:EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; nucleus; cytosol; chromosome, pericentric region Molecular Function:protein dimerization activity; protein C-terminus binding; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; regulation of transcription, DNA-dependent; meiotic cell cycle; regulation of cell differentiation; regulation of growth; mesoderm development; regulation of receptor activity; regulation of DNA replication; inactivation of MAPK activity; second-messenger-mediated signaling; regulation of cell adhesion; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of protein catabolic process; mitotic cell cycle; negative regulation of cell growth; ceramide metabolic process
NCBI Summary:	This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
UniProt Code:	P67775
NCBI GenInfo Identifier:	54038809
NCBI Gene ID:	5515
NCBI Accession:	P67775.1
UniProt Related Accession:	P67775
Molecular Weight:
NCBI Full Name:	Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
NCBI Synonym Full Names:	protein phosphatase 2 catalytic subunit alpha
NCBI Official Symbol:	PPP2CA
NCBI Official Synonym Symbols:	RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha
NCBI Protein Information:	serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Protein Name:	Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Synonym Protein Names:	Replication protein C
Protein Family:	PP2A regulatory
UniProt Gene Name:	PPP2CA
UniProt Entry Name:	PP2AA_HUMAN

	Western blot analysis of extracts of various cell lines, using PPP2CA Rabbit pAb (CAB6702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human stomach using PPP2CA antibody (CAB6702) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using PPP2CA antibody (CAB6702) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using PPP2CA antibody (CAB6702) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug PPP2CA antibody (CAB6702). Western blot was performed from the immunoprecipitate using PPP2CA antibody (CAB6702) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-Phospho-PP2A alpha-Y307 Antibody (CABP1043)	PP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA Kit
Phospho-PPP2CA (Y307) Antibody (PACO02992)	PP2A-alpha Colorimetric Cell-Based ELISA Kit
PPP2CA Antibody (PACO06773)
PPP2CA Antibody (PACO11429)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)