Description
Porcine Inflammation Array (26 targets) (SARB0097)
The Porcine Inflammation Array - 26 Targets (SARB0097) is a comprehensive tool for studying inflammation in porcine samples. This array covers 26 key targets involved in the inflammatory response, allowing for simultaneous analysis of multiple cytokines and chemokines in pigs.With this array, researchers can easily and efficiently study the expression levels of these inflammation-related targets in porcine samples, providing valuable insight into the immune response in pigs. By measuring the levels of these targets, researchers can better understand the mechanisms underlying inflammatory processes in pigs, leading to potential advances in the treatment and management of inflammatory conditions in porcine health.
This array is a valuable tool for studies on inflammation, immune response, and infectious diseases in pigs, offering a high-throughput solution for analyzing multiple targets in a single experiment. Its ease of use, sensitivity, and specificity make it an ideal choice for researchers looking to deepen their understanding of inflammation in porcine models.
| Product Sku: | SARB0097 |
| Size: | 2, 4, or 8 |
| Species Detected: | Porcine |
| Number of Targets Detected: | 26 |
| Gene Symbols: | CCL4, CCL5, CSF2, CXCL8, CXCL9, IFN-BETA, IFNB, IFNB1, IFNG, IGIF|IL10, IL12A, IL13, IL15, IL17, IL17A, IL17F, IL18, IL1A, IL1B, IL1RN, IL21, IL22, IL28B, IL4, IL6, IL8, INTERFERON-BETA-1, INTERFERON-BETA/POIFN-BETA, IRAP1, MIF, MIP1B, SCY4A, SCYA5, TNF, TNFA, TNFSF2 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Eotaxin-1 (CCL11)
| GM-CSF
| IFN alpha
| IFN-beta
| IFN-gamma
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 Ra (IL-1 F3)
| IL-10
| IL-12 p70
|
IL-13
| IL-15
| IL-17A
| IL-17F
| IL-18
|
IL-21
| IL-22
| IL-28B
| IL-4
| IL-6
|
IL-8 (CXCL8)
| MIF
| MIG (CXCL9)
| MIP-1 beta (CCL4)
| RANTES (CCL5)
|
TNF alpha
| ||||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Porcine Inflammation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
