The PP2A Catalytic alpha Antibody (CAB6702) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high specificity for human samples and is validated for use in Western blot applications. By targeting the PPP2CA protein, researchers can investigate its role in cellular processes such as cell cycle regulation, apoptosis, and DNA repair.PP2A is a major serine/threonine phosphatase that plays a crucial role in the regulation of various signaling pathways, making it a significant target for research in cancer, neurodegenerative diseases, and other disorders.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PP2A Catalytic alpha Antibody
SKU:
CAB6702
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Chromosome, Cytoplasm, Nucleus, Centromere, Cytoskeleton, Spindle Pole.
Calculated MW:
36kDa
Observed MW:
36kDa
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
Purification Method
Affinity purification
Gene ID
5515
RRID
AB_2767286
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using PP2A Catalytic α Rabbit pAb (CAB6702) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using PP2A Catalytic α Rabbit pAb (CAB6702) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using PP2A Catalytic α Rabbit pAb (CAB6702) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using PP2A Catalytic α Rabbit pAb (CAB6702) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg PP2A Catalytic α antibody (CAB6702). Western blot was performed from the immunoprecipitate using PP2A Catalytic α antibody (CAB6702) at a dilution of 1:1000.
Immunoprecipitation analysis of 500 μg extracts of Rat brain cells using 3 μg PP2A Catalytic α antibody (CAB6702). Western blot was performed from the immunoprecipitate using PP2A Catalytic α antibody (CAB6702) at a dilution of 1:1000.
