The Recombinant PAKγ Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110815) against PAKγ. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of K562 and IHC analysis of Human breast carcinoma. The target is localized to the cytoplasm, MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane, Nucleus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
Product Name:
Recombinant PAKγ Monoclonal Antibody
SKU:
AGEL6121
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
65-KD, C-t-PAK2, CB422, EC 2.7.11.1, Gamma PAK, Gamma-PAK, hPAK65, Kinase, p21 (CDKN1A) activated kinase 2, p21 (CDKN1A)-activated kinase 2a, p21 activated kinase 2, p21 protein (Cdc42/Rac)-activated kinase 2, p21 protein Cdc42 Rac activated kinase 2, p21-activated kinase, p21-activated kinase 2, p21-activated protein kinase I, p21CDKN1A activated kinase 2, p27, p34, p58, p65PAK, PAK, PAK 2, PAK-2, PAK-2p34, PAK2, PAK65, PAKgamma, S6 H4 kinase, S6/H4 kinase, Serine threonine protein kinase PAK 2, Serine/threonine protein kinase PAK 2
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human PAKγ protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110815
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
PAKγ
Conjugation:
Unconjugated
Observed MW:
58 kDa
Calculated MW:
58 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
Q13177
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
