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Mononuclear Phagocytes in Health and Disease(Post)
Mononuclear phagocytes, essential in immunity and homeostasis, perform diverse roles from phagocytosis to tissue repair, influencing health and disease. Key Takeaways: Mononuclear p
von Willebrand Factor and Cancer(Post)What is von Willebrand Factor? Von Willebrand Factor (VWF) is a crucial glycoprotein that plays a fundamental role in blood clotting and hemostasis. Produced by endot
ELISA Cross-Reactivity: Tagged Standards vs Native Samples(Post)Quick answer A researcher validating two isoform assays — the Human ALT1 (HUFI00557) and ALT2 (HUFI00593) ELISA kits — spiked the ALT2 kit's standard onto the ALT1 kit's plate and saw
RIPA Buffer Recipe - Cell Lysis | Assay Genie(Post)RIPA (Radio Immuno Precipitation Assay) buffer is mostly used when carrying out a western blot or immunoprecipatation assay. A RIPA buffer is used in order to lyse cells and extract protein fro
Keep your Lab Book up to Date(Post)When PhDs and Post-Docs start in new lab they have the best intentions to keep their lab books up to date with notes, new research methods, data, and protocol information. These best efforts us
Necrosis: Types, Causes, Symptoms & Prevention Guide(Post)Cell Biology · PathologyNecrosis: Types, Causes, Mechanisms and How It Is MeasuredNecrosis is cell death accompanied by membrane rupture and release of intracellular contents — which is what makes it
A broccoli juice a day keep the heart attack away(Post)Alex Lloyd PhD Candidate, Dublin Institute of Technology Did you know that roughly 17.5 million people worldwide die annually from Cardiovascular diseases
The Adenosine Axis: How CD39 and CD73 Switch Immunity Off(Post)The most abundant immunosuppressive molecule in a solid tumour is not a protein. It is adenosine — a metabolite, produced from ATP in two enzymatic steps, at concentrations orders of magnitude above
Unconventional T Cells: Gamma Delta, iNKT and MAIT(Page) Transmission of SARS-CoV-2 (COVID-19) via Droplets and Aerosols (Post)Transmission of SARS-CoV-2 (COVID-19) via Droplets and Aerosols What is COVID-19? COVID-19, the disea
Guide to Fluorescence Activated Cell Sorting (FACs): The Latest Technology in Cell Separation(Post)Flow Cytometry · Cell SortingGuide to Fluorescence-Activated Cell Sorting (FACS)Fluorescence-activated cell sorting (FACS) is a specialised form of flow cytometry that physically isolates individual c
Onvatilimab: Redefining the Role of Anti-CD47 in Cancer Research(Post)Quick Facts About OnvatilimabWhat is Onvatilimab?Onvatilimab is a monoclonal antibody targeting CD47, often referred to as the "don't eat me" signal, which is overexpressed on many cance
Western Blot Training: A Comprehensive Guide(Post)Western blotting, a cornerstone technique in molecular biology, biochemistry, and immunogenetics, is pivotal for detecting specific proteins in a sample. Its applications span various scientific f
Inflammasomes: NLRP3, Caspase-1 and IL-1(Page) Guide to Bacterial Transformation: The Science of Genetic Manipulation(Post)Techniques · Molecular CloningGuide to Bacterial Transformation: The Science of Genetic ManipulationBacterial transformation is the process by which bacteria take up foreign DNA — usually a plasmid —
ELISA Plate-to-Plate OD Differences: Same-Lot Troubleshooting(Post)Quick answer When plates from the same lot give very different OD values, the cause is almost always a comparison problem, not a manufacturing problem. The three usual culprits are a t
Biofilms: The Bacteria Invaders You Can't Ignore(Post)If you've ever had a urinary tract infection, you know all about bacteria. These tiny organisms can invade your body and cause all sorts of problems. But what you may not know is tha
Rosopatamab: Unveiling the Potential of Anti-CD47 Therapy in Cancer Research(Post)Quick Facts About RosopatamabWhat is Rosopatamab?Rosopatamab is a monoclonal antibody targeting CD47, a critical immune checkpoint that helps cancer cells evade immune detection. By bloc
ELISA Troubleshooting: High %CV at Low Standards(Post)QUICK ANSWER On a Human I-FABP/FABP2 ColorStep ELISA, the top of the standard curve looked perfect while the three lowest standards showed duplicate %CVs of 37–450% — because their op
Mycoplasma Troubleshooting Tips & FAQ(Page)