Description
c-Jun (Phospho-Thr239)Transcription Factor Activity Assay
The c-Jun Phospho-Thr239 Transcription Factor Activity Assay is a cutting-edge research tool designed for the sensitive and specific detection of c-Jun phosphorylation at Thr239, a key post-translational modification that regulates transcription factor activity. This assay kit offers unparalleled accuracy and reproducibility, enabling researchers to investigate the role of c-Jun in various cellular processes and disease pathways.c-Jun is a critical component of the AP-1 transcription factor complex and plays a crucial role in regulating gene expression, cell proliferation, and apoptosis. Phosphorylation of c-Jun at Thr239 has been implicated in various diseases, including cancer, inflammation, and neurodegenerative disorders, making it an attractive target for therapeutic intervention.
With its high performance and versatility, the c-Jun Phospho-Thr239 Transcription Factor Activity Assay is an invaluable tool for researchers studying signal transduction pathways, gene regulation, and disease mechanisms. This assay kit is suitable for use with a wide range of sample types, including cell lysates, tissue homogenates, and protein extracts, making it an indispensable resource for advancing biomedical research.
| Product Name: | c-Jun (Phospho-Thr239)Transcription Factor Activity Assay |
| Product Code: | TFAB00007 |
| Target: | c-Jun (Phospho-Thr239) |
| Synonyms: | Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie c-Jun (Phospho-Thr239) transcription factor activity assay allows for the detection and qualitative analysis of c-Jun (Phospho-Thr239) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). |
| NCBI Summary: | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P05412 |
| NCBI GenInfo Identifier: | 135298 |
| NCBI Gene ID: | 3725 |
| NCBI Accession: | P05412.2 |
| UniProt Secondary Accession: | P05412,Q6FHM7, Q96G93, |
| UniProt Related Accession: | P05412 |
| Molecular Weight: | |
| NCBI Full Name: | Transcription factor AP-1 |
| NCBI Synonym Full Names: | jun proto-oncogene |
| NCBI Official Symbol: | JUN |
| NCBI Official Synonym Symbols: | AP1; AP-1; c-Jun |
| NCBI Protein Information: | transcription factor AP-1 |
| UniProt Protein Name: | Transcription factor AP-1 |
| UniProt Synonym Protein Names: | Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 |
| Protein Family: | C-Jun-amino-terminal kinase-interacting protein |
| UniProt Gene Name: | JUN |
| UniProt Entry Name: | JUN_HUMAN |
