279 results for 'cold'

RIPA (Radio Immuno Precipitation Assay) buffer is mostly used when carrying out a western blot or immunoprecipatation assay. A RIPA buffer is used in order to lyse cells and extract protein fro

The selection of a secondary antibody is a critical step in the experimental design of various immunodetection methods, including Western Blot, ELISA, immunohistochemistry (IHC), and immunofluores

Tertiary Lymphoid Structures: The Body's Hidden Arsenals in the Fight Against Cancer Imagine a battlefield within the body, where the immune system stands as the last line of defense against the rele

Protein Analysis · ProtocolsWestern Blot Sample Preparation: A Practical GuideMost failed Western blots fail before the gel is loaded. Lysis buffer choice, inhibitor selection, accurate protein quanti

Transforming Growth Factor-Beta (TGF-β) signaling pathway plays a pivotal role in orchestrating various cellular processes, ranging from embryonic development to tissue homeostasis and immune resp

Lab Methods · Flow CytometryFlow Cytometry Protocol: Two Worked Protocols, Controls and 10 Practical TipsFlow cytometry measures individual cells in a fluid stream at hundreds per second, reporting si

Quick Facts About CanakinumabWhat is Canakinumab?Canakinumab is a human monoclonal antibody that targets interleukin-1 beta (IL-1β), reducing inflammation associated with various disease

For decades, the battle against cancer has focused on the cellular 'soldiers' and the genetic 'blueprints' within them. Yet, a vast, invisible world of communication exists between these cells—a secr

COVID-19 Testing Results in 15 Minutes Accurate detection of IgM and IgG patient-generated antibodies with the rapid, point

Quick Facts About TiragolumabWhat is Tiragolumab?Tiragolumab is an anti-TIGIT monoclonal antibody developed to enhance immune responses against cancer cells. It is currently under invest

Apoptosis, or programmed cell death, is a fundamental process that plays a critical role in the development and maintenance of healthy tissues. Central to this process are caspases, a family of cy

By Shane Houston, PhD Candidate Queen’s University Belfast Cancer is an age old adversary of the human race. This out-of-control growth of abnormal cells has been a shadow over human health t

Immunology · Cell IsolationPeripheral Blood Mononuclear Cells (PBMCs): Composition, Isolation and UseMononuclear cells are the leukocytes with a single, unlobed nucleus — lymphocytes and monocytes. Is

Workplace Testing for COVID-19 Corporate Testing for COVID-19 The impact of COVID-19 on the econom

Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bact

Cryopreservation of stimulated T cells is a common technique in immunology research, allowing for long-term storage while maintaining their functional viability. Stimulated T cells—especi

Progression of the JAK STAT Signalling Pathway Cellular communication is a complex network of molecular interactions that govern various physiological processes. Among the many signal

Western Blot Protocol & Troubleshooting Guide | 14 Steps + 101 Tips | Assay Genie

Immunodetection · ProtocolsSecondary Antibody Incubation: Times, Conditions and TroubleshootingThe secondary antibody step determines how much signal you get and how much background comes with it. Inc

Techniques · Gel ElectrophoresisAgarose vs Polyacrylamide: A Comparative AnalysisAgarose and polyacrylamide are the two workhorse gel matrices of electrophoresis. Agarose gels resolve larger DNA and R

Sweet Deception: How a New Antibody Therapy Unmasks Pancreatic Cancer Sweet Deception: How a New Antibody Therapy Unmasks Pancreatic Cancer Pancreatic canc

What You Need to Know About MilatuzumabWhat is Milatuzumab?Milatuzumab is a humanized monoclonal antibody targeting CD74, a molecule involved in antigen presentation and cell signaling.W

Cell Culture · ProtocolsPassaging Hybridoma Cells: A Detailed GuideHybridomas are productive but temperamental. They grow in suspension, are sensitive to shear and to overgrowth, and — the problem tha

Quick Facts About TarextumabWhat is Tarextumab?Tarextumab is a novel therapeutic antibody designed to target the Notch signaling pathway, which plays a crucial role in tumor progression

Rapid Antibody & Antigen tests available from Assay Genie

Imagine a living drug, engineered from a patient's own immune system, that can hunt down and destroy cancer cells with remarkable precision. This isn't science fiction; it's the reality of Chimeric An

Imagine a high-stakes game of hide-and-seek where the seeker is your immune system and the hider is a master of disguise: cancer. For decades, we believed the 'wanted' posters for tumor cells were li

By Dr. Lisa Dwane, Postdoctoral Researcher, Royal College of Surgeons in Ireland The first link between estrogen and breast cancer was made in the late 1

For decades, the primary strategy in cancer treatment has been to trigger apoptosis, a form of programmed cell death, in malignant cells. However, many cancers develop resistance to apoptosis-inducin

What if our first line of immune defense, the innate immune system, could learn from past encounters to fight future battles more effectively? For decades, we believed this was the exclusive domain of

Imagine a microscopic sniper, capable of delivering a lethal blow to a single cancer cell while leaving its healthy neighbors virtually untouched. This is the promise of targeted alpha therapy, a rev

Novel Gene Therapies for Cystic Fibrosis Cystic Fibrosis Cystic fibrosis (CF) is an autosomal recessive, life-limiting disease resulting from gene mutations that encode the cystic fibr

Lab Methods · CentrifugationRPM to G-Force (RCF) Conversion: Formula, Calculator Method and Common ErrorsA protocol that says “spin at 3,000 rpm” is incomplete, because the force a sample experiences

Cell Signalling · InflammationMAPK Signalling in Inflammatory Cytokine PathwaysMAPK cascades convert receptor engagement into changes in gene expression, and in inflammation they do it in both directi

Cell Death · BiomarkersCleaved Caspase-3: Activation, Function and Detection in ApoptosisCaspase-3 sits at the point where apoptotic signalling becomes irreversible. It is made as an inactive proenzym

Cell Biology · PathologyNecrosis: Types, Causes, Mechanisms and How It Is MeasuredNecrosis is cell death accompanied by membrane rupture and release of intracellular contents — which is what makes it

In Vivo Methods · ProtocolsMouse Injection Guide: Intraperitoneal, Subcutaneous, Intramuscular & Intravenous RoutesRoute choice and injection technique determine how much of a dose actually reaches it

BRIEF HISTORY OF THE “GREEN” EVOLUTION OF OXYGEN AND CARBON DIOXIDE WITHIN OUR ATMOSPHERE Million years ago carbon dioxide (CO2) levels used to be conside

Toll-Like Receptors The innate immune response has come under the spotlight in recent years due to its central role in propagating the pathogen