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Immuno-Biomaterial Development | Assay Genie(Post)
Background I am a 3rd year PhD student in Trinity College Dublin and my project is very much an interdisciplinary one. I’m very lucky to get to work between two great labs, Dr. Aisling Dunn
Oxidative Stress Assays(Page)Oxidative Stress Assay Kits | ROS, Antioxidant & Glutathione | Assay Genie
PD-1/PD-L1 Checkpoint Signalling: Pathway, Function and Assays(Post)PD-1 does not switch a T cell off; it moves the threshold. Engagement of PD-1 by PD-L1 recruits a single phosphatase into the immunological synapse, and that one recruitment event is enough to collap
Cell Viability, Cytotoxicity & Proliferation Assays(Page)Cell Viability, Cytotoxicity & Proliferation Assay Kits | Assay Genie
Comprehensive Guide to Mononuclear Cells(Post)Immunology · Cell IsolationPeripheral Blood Mononuclear Cells (PBMCs): Composition, Isolation and UseMononuclear cells are the leukocytes with a single, unlobed nucleus — lymphocytes and monocytes. Is
Passaging Hybridoma Cells: A Detailed Guide(Post)Cell Culture · ProtocolsPassaging Hybridoma Cells: A Detailed GuideHybridomas are productive but temperamental. They grow in suspension, are sensitive to shear and to overgrowth, and — the problem tha
Insulin Signaling and RTK: An Overview(Post)Cell Signalling · MetabolismInsulin Signalling & Receptor Tyrosine KinasesInsulin controls metabolism and growth through the insulin receptor — a receptor tyrosine kinase that branches into the
SLAMF7: Activating Natural Killer Cells for Potent Anti-Cancer Responses(Post)Introduction to SLAMF7 in Cancer Immunotherapy SLAMF7, also known as Signaling Lymphocytic Activation Molecule F7, is a surface receptor found on natural killer (NK) cells, T cells, plasm
JAK-STAT Signalling and Cytokines: The What, How, and Why(Post)Cell Signalling · CytokinesJAK-STAT Signalling: Mechanism, Regulation and InhibitorsJAK-STAT is the shortest route from a cytokine outside the cell to a change in gene transcription — receptor engagem
How to Thaw Cells: Best Practices for Cell Culture Success(Post)Cell culture techniques are fundamental to biomedical research, providing crucial insights into cellular functions, drug discovery, and disease mechanisms. A critical step in cell culture is the p
Western Blot Training: A Comprehensive Guide(Post)Western blotting, a cornerstone technique in molecular biology, biochemistry, and immunogenetics, is pivotal for detecting specific proteins in a sample. Its applications span various scientific f
How to Choose a Secondary Antibody(Post)The selection of a secondary antibody is a critical step in the experimental design of various immunodetection methods, including Western Blot, ELISA, immunohistochemistry (IHC), and immunofluores
Innate Lymphoid Cells: ILC1, ILC2 & ILC3(Page) Dual PD-1/PD-L2 Blockade: Expanding the Horizons of Cancer Immunotherapy(Post)Introduction to PD-1, PD-L1, and PD-L2 in Cancer ImmunotherapyThe PD-1/PD-L1 immune checkpoint pathway has been instrumental in cancer immunotherapy, with PD-1 (programmed death-1) inhibi
The Role of Claudins in Cancer (Post)Introduction Claudins are a family of proteins that play a critical role in the formation of t
Understanding CD16: A Comprehensive Overview(Post)CD16, also known as FcγRIII (Fc gamma receptor III), plays a crucial role in the immune system, serving as a receptor for the Fc region of immunoglobulin G (IgG). This receptor is a key player in
Osteoimmunology: The RANKL-OPG Axis & Bone Erosion(Page) Understanding CD16: A Comprehensive Overview(Post)CD16, also known as FcγRIII (Fc gamma receptor III), plays a crucial role in the immune system, serving as a receptor for the Fc region of immunoglobulin G (IgG). This receptor is a key player in
Carbon Dioxide sensing in Immune Cells(Post)BRIEF HISTORY OF THE “GREEN” EVOLUTION OF OXYGEN AND CARBON DIOXIDE WITHIN OUR ATMOSPHERE Million years ago carbon dioxide (CO2) levels used to be conside
No Ligand, No Kinase, No Problem(Post)HER2 has no ligand. HER3 has no kinase. Together they are the most potent signalling pair in human biology. That single sentence explains most of what is strange about this receptor family and all of