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ELISA Troubleshooting: High %CV at Low Standards(Post)
QUICK ANSWER On a Human I-FABP/FABP2 ColorStep ELISA, the top of the standard curve looked perfect while the three lowest standards showed duplicate %CVs of 37–450% — because their op
The Complement System - pathways & function in the immune system(Post)The Complement System Function Gone are the days when the complement system was considered solely a companion of its impressive adaptive immune system counte
Diabetic Retinopathy: premature cellular senescence and microvascular dysfunction(Post)By Pietro Maria Bertelli, PhD Candidate, Queens University Belfast Introduction Eye complications are very frequent in patients with diabetes, and thi
Nod-Like Receptor Signaling Pathway: A Keystone in Innate Immunity(Post)The innate immune system, a primary line of defense against pathogens, comprises various cellular and molecular mechanisms. Among these, the Nod-like Receptor (NLR) signaling pathways play a criti
Tezepelumab: A Breakthrough in Severe Asthma Treatment and Research(Post)Quick Facts About TezepelumabWhat is Tezepelumab?Tezepelumab is a monoclonal antibody designed to inhibit thymic stromal lymphopoietin (TSLP), a key driver of airway inflammation in seve
Oxidative Stress: Causes, Biomarkers & Disease(Post)Oxidative Stress Definition Oxidative stress is a condition that results when the body produces or consumes more reactive oxygen species (ROS) than it can neutraliz
Avoid Reagent Risk: Polyclonal vs Monoclonal Antibodies for Researchers(Post)For reproducible, isoform-specific detection, choose a monoclonal antibody; for robust detection across multiple epitopes or faster turnaround, choose a polyclonal antibody. The real tradeoff is epit
IQGAP1 signaling review(Post)IQGAP1: IQ-motif containing GTPase-activating-like protein-1 reviewIQ motif-containing GTPase-activating-like protein-1 (IQGAP1) is a 190 KDa protein that belongs to a conserved family of sc
Cell Viability, Cytotoxicity & Proliferation Assays(Page)Cell Viability, Cytotoxicity & Proliferation Assay Kits | Assay Genie
Bullying in the Laboratory and workplace(Post)The lab can be one of the greatest places in the world to make live long friends. Spending countless hours in a tissue culture room late into the middle of the night, sharing similar frustratio
What is the difference between PBS and dPBS?(Post)Lab Buffers · ProtocolsPBS vs DPBS: What’s the Difference?PBS and DPBS are the everyday isotonic, pH-balanced salt solutions used to wash, dilute and transport cells and proteins. This guide bre
MAPK Signaling: Unraveling the Pathway of Mitogen Stimulation(Post)Cell Signalling · Kinase CascadesMAPK Signaling: The Pathway of Mitogen StimulationThe mitogen-activated protein kinase (MAPK) pathway is one of the most important signalling cascades in biology, conv
Phosphatases and PTP1B – Mini review(Post)IntroductionThe human kinome is estimated to contain 518 genes, in comparison to the estimated 180 genes that comprise the phosphatome (Arena et al., 2005). These kinase genes represent a signif
Tumour Inflammation & Signaling | Assay Genie(Post)By Charlotte O’Donnell PhD Inflammation and Cancer The link between inflammation and cancer is well established, with infl
IHC Buffers & Recipes - Mandarin(Page)免疫组织化学缓冲液与配方(IHC,中文) | Assay Genie
An Overview of the glycoprotein GM-CSF(Post)This article breaks down the glycoprotein Granulocyte-macrophage colony-stimulating factor (GM-CSF) detailing its structure, function and therapeutic uses. It also describes its role in imm
The double-edged sword in prostate cancer therapy(Post)By Zoe Angel, PhD Student, Ulster University Prostate cancer is now the commonest cancer in men and it is the fifth leading cause of death (1). Researchers h
Ionotropic Glutamate Receptors: Gateways to Neural Communication(Post)Ionotropic glutamate receptors (iGluRs) are pivotal in the fast excitatory synaptic transmission in the mammalian central nervous system (CNS). These receptors are not only crucial for normal brai
Myeloid Checkpoints: CD47-SIRPa, LILRB and Siglec-10(Page) Indusatumab: Advancing Anti-CD47 Cancer Research with Biosimilar Innovations(Post)Quick Facts About IndusatumabWhat is Indusatumab?Indusatumab is an experimental antibody therapy targeting CD47, a protein that enables cancer cells to evade immune destruction.How does