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Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bact

Cell Culture · ProtocolsCell Synchronisation Methods: Protocols for G1, S, G2 and M Phase ArrestAn asynchronous culture contains cells at every stage of the cycle at once, so any measurement is an ave

Lab Methods · Flow CytometryFlow Cytometry Protocol: Two Worked Protocols, Controls and 10 Practical TipsFlow cytometry measures individual cells in a fluid stream at hundreds per second, reporting si

Site-Directed Mutagenesis | Protocol, Kits & Troubleshooting | Assay Genie

Biochemistry · Cell SignallingPhosphorylation: Types, ATP Synthesis and Cell SignallingAdding a phosphate group is how cells both store energy and switch proteins on and off. The same chemistry underl

Workplace Testing for COVID-19 Corporate Testing for COVID-19 The impact of COVID-19 on the econom

What is CD31? CD31, also known as PECAM-1, was originally described as a cell surface antigen present on platelets and megakaryocytes. CD31 is

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The mast cell is loaded long before the allergen arrives. IgE bound to FcεRIα is not waiting to be recruited the way an antibody waits for antigen in a serum assay — it is already installed on the ce

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant

Cell culture techniques are fundamental to biomedical research, providing crucial insights into cellular functions, drug discovery, and disease mechanisms. A critical step in cell culture is the p

If your knockout showed a type 2 phenotype and you attributed it to Th2 cells, there is a reasonable chance you were looking at something else. Innate lymphoid cells run T-bet, GATA3 and RORγt — not