IHC Buffers & Recipes

Immunohistochemistry (IHC) Buffers & Recipes

Outlined below you will find a selection of immunohistochemistry buffers and recipes including: fixation buffers, buffers for heat-induced epitope retrieval (HIER), enzymatic detection buffers, permeabilization and blocking buffers.

Sample Fixation Buffers

Zenker’s Solution

Use Zenker’s solution for bloody samples such as spleen and connective tissue

Reagent Amount
Mercuric Chloride
5 g
Potassium Dichromate
2.5 g

ddH20

100 ml

*Heat and cool. Add 5 ml of glacial acetic acid just before use. Never use metal forceps when handling tissues fixed in Zenker Solution.

Helly’s Solution

Helly’s solution can be used on blood forming organs such as bone marrow, liver and spleen.

Reagent Amount
Mercuric Chloride
5 g
Potassium Dichromate
2.5 g

ddH20

100 ml

*Heat and cool. Add 5 ml of formaldehyde just before use.

Bouin’s Fixative

Bouin’s Fixative should be used when soft tissue structures need to be preserved.

Reagent Amount
Picric Acid (Saturated)
75 ml
Formaldehyde (37-40%)
25 ml
Glacial Acetic Acid
5 ml

Carnoy’s Solution

Carnoy’s solution should be used for the fixation of DNA and RNA.

Reagent Amount
Ethanol (100%)
60 ml
Chloroform
30 ml
Glacial Acetic Acid
10 ml

Zinc Formalin

  1. Make a 0.1M Tris Buffer, pH 7.4