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CD39: A Novel Target to Overcome Immunosuppression in Cancer(Post)
Introduction to CD39 and Cancer Immunosuppression CD39 is an ectonucleotidase enzyme that plays a crucial role in generating adenosine, a powerful immunosuppressive molecule within the tu
NLRP3 Inflammasome and Pyroptosis: Pathway, Function and Assays(Post)The inflammasome is one of the few innate pathways that destroys the cell it activates. A macrophage that assembles NLRP3 has committed to a decision it cannot reverse: a priming signal through TLR4
Types of PCR – 7 Methods, Principles & Applications(Post)Molecular Biology · Techniques Guide Types of PCR – 7 Methods, Principles & Applications PCR stands as a cornerstone in molecular biology, ena
Human Multiplex ELISA Panels(Page)Human Multiplex Cytokine ELISA Panels | Assay Genie
Combien coûte réellement un anticorps de recherche ?(Post)Combien coûte réellement un anticorps de recherche ? Prévoyez un budget de 130 à 2 500 dollars par anticorps selon le format et l’application visée, mais ce chiffre ne raconte qu’une partie de l’hist
TMB Incubation Time in ELISA: Why It Makes or Breaks Results(Post)Quick answer A researcher ran a high-sensitivity porcine insulin sandwich ELISA on EDTA plasma and saw main-run duplicate CVs as high as 29.6% (kit spec: intra-assay CV <8%) togethe
Targeting Immune Checkpoints as Cancer Therapy(Post)In recent years, the field of oncology has witnessed a paradigm shift with the advent of immunotherapy, a treatment modality that harnesses the body's immune system to combat cancer. Among the mos
GPCRs (G Protein Coupled Receptors): A Guide(Post)Cell Signalling · Receptor BiologyGPCRs (G Protein-Coupled Receptors): A Complete GuideG protein-coupled receptors are the largest family of cell-surface receptors in the human genome and the ta
Immunometabolism – A therapeutic perspective(Post)What is Immunometabolism? Immunometabolism is an exponentially growing, multi-disciplinary field of research aiming at deciphering the dynamic cellular and mole
Competitive vs Sandwich ELISA: Should You Subtract the Blank?(Post)Quick answer: A researcher subtracted the blank from a competitive ELISA (a Serotonin kit) and every result turned negative. In a competitive assay the signal is inversely proportional to
Mouse Multiplex ELISA Panels(Page)Mouse Multiplex ELISA & Cytokine Assay Panels | Assay Genie
TGF-Beta SMAD Signalling Pathway: Function, Readouts and Assays(Post)Almost all of the TGF-β in a tissue is already there, and almost none of it is active. That single fact reorganises how the pathway should be studied. Unlike most cytokines, TGF-β is deposited into t
RIPA Buffer Recipe - Cell Lysis | Assay Genie(Post)RIPA (Radio Immuno Precipitation Assay) buffer is mostly used when carrying out a western blot or immunoprecipatation assay. A RIPA buffer is used in order to lyse cells and extract protein fro
Typical Workflow of CRISPR-Cas9 Genome Editing(Post)Molecular Biology · ProtocolsTypical Workflow of CRISPR-Cas9 Genome EditingA CRISPR experiment is only as good as the steps around the cut. This guide walks the full workflow — designing and scoring a
COVID-19's Impact on Brain Aging and Cognitive Decline(Post)Impact of COVID-19 on Brain Aging and Cognitive DeclineThe COVID-19 pandemic has had profound effects on various aspects of human health, including mental health. A recent study involving nearly 1,000
What is the difference between PBS and dPBS?(Post)Lab Buffers · ProtocolsPBS vs DPBS: What’s the Difference?PBS and DPBS are the everyday isotonic, pH-balanced salt solutions used to wash, dilute and transport cells and proteins. This guide bre
ELISA Troubleshooting: When Standards Read Below the Blank(Post)Quick answer On the GDF-6 (Growth Differentiation Factor 6) ELISA Kit, a researcher saw an elevated blank (OD ≈ 0.41) with the two lowest standards reading at or below it, and no signa
CD8+ T Cells: Understanding the Role of Cytotoxic T Cells in Immunity(Post)Immunology · T LymphocytesCD8+ T Cells: Cytotoxic Mechanisms, Memory and ExhaustionCD8+ T cells kill infected and transformed cells directly, and they do it with a specificity nothing else in the immu
PD-L1 in Cancer Immunotherapy: Key Insights(Post)PD-L1 in Cancer Immunotherapy: Insights from Recent ResearchRecent advancements in cancer immunotherapy have underscored the critical role of programmed death-ligand 1 (PD-L1) in mediating immune evas
Leukocytes : Basics to Diseases(Post)Only 1% of our blood is made up of white blood cells, but they have a significant impact. Alternatively referred to as leukocytes, white blood cells. We are shielded from disease and