-
MAPK Signaling: Unraveling the Pathway of Mitogen Stimulation(Post)
Cell Signalling · Kinase CascadesMAPK Signaling: The Pathway of Mitogen StimulationThe mitogen-activated protein kinase (MAPK) pathway is one of the most important signalling cascades in biology, conv
CIDE: The New Bioinformatics Engine Unmasking Secreted Proteins for Precision Immunotherapy(Post)For decades, the battle against cancer has focused on the cellular 'soldiers' and the genetic 'blueprints' within them. Yet, a vast, invisible world of communication exists between these cells—a secr
Transduction vs Transfection: Understanding Gene Delivery Techniques(Post)Techniques · Gene DeliveryTransduction vs Transfection: Understanding Gene Delivery TechniquesTransduction and transfection are the two main routes for introducing genetic material into cells. Transdu
The Battle of Antibiotics: Penicillin vs. Streptomycin(Post)Microbiology · AntibioticsPenicillin vs Streptomycin: The Battle of AntibioticsPenicillin and streptomycin are two of the most important antibiotics ever discovered — and they work in completely diffe
CD4 T-Helper Differentiation: Th1, Th2, Th17, Treg and Tfh(Post)The antigen tells a CD4 T cell to respond; the cytokine tells it what kind of response to make. A naive CD4⁺ T cell leaving the thymus is uncommitted. Within about seventy-two hours of engaging TCR/C
ELISA Troubleshooting: Total Protein Normalization in Lysates(Post)QUICK ANSWER A researcher ran a panel of EMT-marker ELISA kits (E-cadherin, claudin-1, N-cadherin, vimentin, ZEB1, TWIST1) on breast cell-line lysates and found E-cadherin reading "pos
The common characteristics of cancer | Assay Genie(Post)By Shane Houston, PhD Candidate Queen’s University Belfast Cancer is an age old adversary of the human race. This out-of-control growth of abnormal cells has been a shadow over human health t
In Vivo Cell Depletion: Antibody Targets, Fc Mechanisms and Dosing(Post)A depletion experiment is only as good as the depletion you can prove. Injecting a functional-grade antibody and assuming the population is gone is the most common failure mode in in vivo immunology.
How do different types of vaccines work? (Post)What is a vaccine? A vaccine is a pharmacological product that gives the recieving patient's immune system a chance to learn how to fight infection with decreased risk of illness. Vacci
Protease vs Peptidase: Understanding Enzymatic Digestion(Post)Enzymology · Protein DegradationProtease vs Peptidase: Understanding the DifferenceProteases and peptidases are the enzymes that cleave peptide bonds — breaking proteins down into peptides and peptide
Validate Western Blot Antibodies: Datasheet Checks Labs Should Ask(Post)Validate Western Blot Antibodies: Datasheet Checks Labs Should Ask Reliable results come from antibodies validated specifically for denatured western blot conditions: recombinant monoclonals or well-
TCA Cycle Assays(Page) Trained Immunity: Reprogramming Innate Immune Memory for Future Health(Post)What if our first line of immune defense, the innate immune system, could learn from past encounters to fight future battles more effectively? For decades, we believed this was the exclusive domain of
Beta Oxidation of Fatty Acids(Post)Metabolism · Biochemistry GuideBeta-Oxidation of Fatty AcidsBeta-oxidation is the mitochondrial pathway that breaks fatty acids down into acetyl-CoA for energy. This guide covers where it happen
miRNAs as therapeutics and biomarkers for pancreatic cancer(Post)Pancreatic cancer survival rates Despite recent advances in our understanding of this disease, pancreatic cancer is one of the world’s most lethal malignan
The Role of Tumor Suppressor Genes in Cancer: Knudson Hypothesis & Oncogenes(Post)Cancer Biology · GeneticsTumour Suppressor Genes: Roles, Examples & CancerTumour suppressor genes are the brakes on cell growth — they restrain proliferation, repair damaged DNA and trigger apoptosis
Immune Cell 'Bloodhounds': Engineering Metabolite-Sensing Receptors to Infiltrate Solid Tumors(Post)Imagine an elite squad of soldiers sent to neutralize a hidden fortress, only to find themselves wandering aimlessly in a thick, disorienting fog just outside the gates. This is the current reality f
MHC Class I vs Class II: Key Differences & Functions [Guide](Post)Immunology Guide MHC Class I vs Class II: Key Differences & Functions [Guide] Major Histocompatibility Complex molecules present antigens to T
Beyond Burnout: How T Cell Exhaustion Hijacks Cancer Immunity and What We Can Do About It(Post)Imagine a highly trained soldier, elite and effective, sent to the front lines of a relentless war. Day after day, they fight without rest, their supplies dwindle, and the enemy never stops coming. Ev
Transformation Protocol – Sample Transformation Protocols(Post)Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bact