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Guide to Bacterial Transformation: The Science of Genetic Manipulation(Post)
Techniques · Molecular CloningGuide to Bacterial Transformation: The Science of Genetic ManipulationBacterial transformation is the process by which bacteria take up foreign DNA — usually a plasmid —
ELISA Plate-to-Plate OD Differences: Same-Lot Troubleshooting(Post)Quick answer When plates from the same lot give very different OD values, the cause is almost always a comparison problem, not a manufacturing problem. The three usual culprits are a t
Cell Signalling – Mini Review(Post)Cell Signalling Cell signalling pathways have an important role in integrating a plethora of extracellular and intracellular signals to produce a controlled optimal o
Polo-like kinase (Plk1) substrates and mitosis(Post)Polo-like kinase (Plk1) Polo-like kinase (Plk1), is a serine/threonine kinase that is active during mitosis (Hamanaka et al., 1995). Its name is derived from its origi
PI3K / AKT / mTOR Signalling in T Cells(Page) Colorectal Cancer Review (CRC) - Assay Genie(Post)By Charlotte O’Donnell PhD Cancer Carcinogenesis is a multi-step process that develops through epigenetic changes and muta
Microglial Functions: Immune Response + Neuroinflammation(Post)Key Takeaways: Microglia are immune cells in the CNS, originating from myeloid precursor cells. They surveil the CNS, respond to changes, and maintain neural health. Microglia exist in diff
Types of ELISA: Direct, Indirect, Sandwich & Competitive Compared(Post)Written by Seán Mac Fhearraigh, PhD • Updated: 19 May 2026 • ~9 min read Quick Answer The five main types of ELISA are sandwich, competitive, direct, indirect and multiplex. Sandwich ELISA is
ST2 and IL33(Post)ST2, a member of the IL-1R Subfamily ST2 is a member of the IL-1R subfamily and was identified in 1989 [1]. The ST2 gene is located on chromoso
C-Reactive Protein (CRP): Function, Levels & What They Mean(Post)Biomarkers · InflammationC-Reactive Protein (CRP): Structure, Function and Clinical UseCRP is the most widely measured marker of inflammation in medicine — fast to rise, fast to fall, and entirely non
Deprotonation: Unveiling the Chemistry Behind It(Post)Chemistry · Acid-BaseDeprotonation: Mechanism, pKa and Choosing the Right BaseDeprotonation is the removal of a proton from a molecule to give its conjugate base. Whether it happens, and how completel
Bococizumab: Mechanism, Clinical Applications, and Biosimilars in Research(Post)Quick Facts About BococizumabWhat is Bococizumab?Bococizumab is a monoclonal antibody that targets PCSK9, a protein involved in cholesterol regulation.How Does Bococizumab Work?It inhibi
Angiotensin Pathways: Unlocking the Secrets to Blood Pressure Regulation and Beyond(Post)The angiotensin pathway is a pivotal hormonal system that plays a crucial role in regulating blood pressure and maintaining fluid and electrolyte balance within the body. This complex biochemical
Unveiling the Potential of Cy3 Wavelength: Illuminating the Path to Advanced Biomedical Imaging(Post)In the realm of biomedical imaging, the quest for precision and clarity drives researchers to explore innovative technologies. One such breakthrough is the Cy3 wavelength, a powerful tool revol
In the Thymus, Co-stimulation Kills(Post)In the periphery, co-stimulation rescues a T cell. In the thymus, the same molecules kill it. A peripheral T cell that engages its receptor without CD80 and CD86 becomes anergic; engage them and it r
Molecular Biology(Page)Molecular Biology Tools, PCR & Cloning Kits | Assay Genie
Mucosal Homing and Tissue-Resident Memory(Page) Praluzatamab: Unveiling the Promise of CD47-Targeted Therapy in Cancer Research(Post)Quick Facts About PraluzatamabWhat is Praluzatamab?Praluzatamab is an experimental monoclonal antibody that targets CD47, a “don’t eat me” signal used by cancer cells to evade immune des
Ferroptosis: A New Frontier in Cancer Therapy(Post)For decades, the primary strategy in cancer treatment has been to trigger apoptosis, a form of programmed cell death, in malignant cells. However, many cancers develop resistance to apoptosis-inducin
NLRP3 Inflammasome and Pyroptosis: Pathway, Function and Assays(Post)The inflammasome is one of the few innate pathways that destroys the cell it activates. A macrophage that assembles NLRP3 has committed to a decision it cannot reverse: a priming signal through TLR4