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LPS ELISA Troubleshooting: The Importance of Sample Nature(Post)
Quick answer A researcher measuring circulating lipopolysaccharide (LPS) in human serum with our LPS ELISA saw almost no color, even after 30 minutes with substrate. Samples had been test
High-Fidelity DNA Polymerase(Page) ELISA Troubleshooting: High %CV at Low Standards(Post)QUICK ANSWER On a Human I-FABP/FABP2 ColorStep ELISA, the top of the standard curve looked perfect while the three lowest standards showed duplicate %CVs of 37–450% — because their op
Une courbe par plaque pour la courbe standard ELISA au laboratoire(Post)Une courbe par plaque pour la courbe standard ELISA au laboratoire Une courbe standard ELISA convertit les densités optiques mesurées en concentrations exploitables, à partir d’une série d’étalons de
엘리사 버퍼 및 레시피(Page) Avant le premier pipetage : plan expérimental ELISA, modèles copiables(Post)Un plan expérimental ELISA fiable repose sur six éléments non négociables: des contrôles complets (blanc, négatif, positif, matrice), des étalons pour la courbe de calibration, une série de diluti
ELISA Plate-to-Plate OD Differences: Same-Lot Troubleshooting(Post)Quick answer When plates from the same lot give very different OD values, the cause is almost always a comparison problem, not a manufacturing problem. The three usual culprits are a t
ELISA Troubleshooting: A Low Standard Curve and Samples Reading Off-Scale(Post)Quick answer On the Periaxin (PRX) ELISA, a researcher found every serum sample reading above the top standard — optical density (OD) around 2.8–3.0 at 450 nm, well beyond the highest
TMB Incubation Time in ELISA: Why It Makes or Breaks Results(Post)Quick answer A researcher ran a high-sensitivity porcine insulin sandwich ELISA on EDTA plasma and saw main-run duplicate CVs as high as 29.6% (kit spec: intra-assay CV <8%) togethe
Optimiser un ELISA : les réglages qui changent vraiment le signal(Post)Optimiser un ELISA : les réglages qui changent vraiment le signal Pour améliorer immédiatement la précision d’un test ELISA, priorisez trois leviers dans l’ordre : la validation et la titration des a
Converting RPM to G-force in Centrifugation(Post)Lab Methods · CentrifugationRPM to G-Force (RCF) Conversion: Formula, Calculator Method and Common ErrorsA protocol that says “spin at 3,000 rpm” is incomplete, because the force a sample experiences
Sandwich ELISA: Step-by-Step Protocol & Troubleshooting(Post)Written by Seán Mac Fhearraigh, PhD • Updated: 19 May 2026 • ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant
3D Organoid Cell Viability Assay: Performance Assessment in Human Colon Organoids(Post)The 3D Organoid Cell Viability Assay (AKES081) demonstrated high sensitivity and low cytotoxicity, making it ideal for long incubation studies (24-48 hours) and high-throughput applicati
Immunoassays(Page)Immunoassays: ELISA, Multiplex & ELISpot Kits | Assay Genie
Cellular Respiration: Stages, ATP Production & Pathway Guide(Post)Metabolism · Biochemistry Guide Cellular Respiration: Stages, ATP Production & Pathway Guide How cells convert glucose and oxygen into ATP acr
Mouse Injection Guide: Intraperitoneal, Subcutaneous, Intramuscular & Intravenous Routes(Post)In Vivo Methods · ProtocolsMouse Injection Guide: Intraperitoneal, Subcutaneous, Intramuscular & Intravenous RoutesRoute choice and injection technique determine how much of a dose actually reaches it
3D Respiratory Organoids Cell Viability in a High-throughput 384-well Plate Assay(Post)The Assay Genie 3D Organoid Cell Viability Assay (AKES081) provided superior results compared to ThermoScientific PrestoBlue, maintaining over 100% viability at all time points in untrea
ELISA Troubleshooting: When Standards Read Below the Blank(Post)Quick answer On the GDF-6 (Growth Differentiation Factor 6) ELISA Kit, a researcher saw an elevated blank (OD ≈ 0.41) with the two lowest standards reading at or below it, and no signa
Évitez la rupture de réactifs en 6 actions pour gestionnaires labo(Post)Évitez la rupture de réactifs en 6 actions pour gestionnaires labo Pour éviter la majorité des ruptures de réactifs, auditez votre inventaire critique, fixez des seuils de commande calculés sur le dé
DNA Assembly Cloning(Page)Featured
Human CAT (Catalase) ELISA Kit (HUFI07676)
- Product Code:
- HUFI07676
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 9.375 pg/mL
- Range:
- 15.625-1000 pg/mL
Human MICA ELISA Kit (HUFI00015)
- Product Code:
- HUFI00015
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 46.875 pg/mL
- Range:
- 78.125-5000 pg/mL
Fatty Acid Oxidation (FAO) Assay Kit (BR00001)
- Product Code:
- BR00001
- Instrument:
- Microplate Reader
Anti-Mouse IFNAR-1 In Vivo Antibody - Low Endotoxin (IVMB0202)
- Product Code:
- IVMB0202
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- MAR1-5A3
- Protein:
- IFNAR1
- Isotype:
- Mouse IgG1
Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin (IVMB0148)
- Product Code:
- IVMB0148
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- PC61
- Protein:
- CD25
- Isotype:
- Rat IgG1 lambda
Anti-Mouse NK1.1 In Vivo Antibody - Low Endotoxin (IVMB0100)
- Product Code:
- IVMB0100
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- PK136
- Protein:
- NK1.1
- Isotype:
- Mouse IgG2a kappa
![Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214372/569320/anti-mouse-pd-1-cd279-rmp1-14-in-vivo-antibody-low-endotoxin__18202.1743527968.jpg?c=2 "Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody - Low Endotoxin")
Anti-Mouse PD-1 Antibody [RMP1-14] In Vivo-Low Endotoxin (IVMB0037)
- Product Code:
- IVMB0037
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- RMP1-14
- Protein:
- PD-1
- Isotype:
- Rat IgG2a kappa
LSAMP Rabbit Polyclonal Antibody (CAB14248)
- Product Code:
- CAB14248
- Antibody Type:
- Polyclonal Antibody
MCM4 Rabbit Polyclonal Antibody (CAB13513)
- Product Code:
- CAB13513
- Antibody Type:
- Polyclonal Antibody
VEGF Rabbit Polyclonal Antibody (CAB12303)
- Product Code:
- CAB12303
- Antibody Type:
- Polyclonal Antibody
MDA ELISA Kit (UNFI0048)
- Product Code:
- UNFI0048
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 4.688 ng/mL
- Range:
- 7.813-500 ng/mL
Dopamine ELISA Kit (UNFI0013)
- Product Code:
- UNFI0013
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 0.938 ng/mL
- Range:
- 1.563-100 ng/mL
Serotonin ELISA Kit (UNFI0018)
- Product Code:
- UNFI0018
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 0.938 ng/mL
- Range:
- 1.563-100 ng/mL
PGE2 ELISA Kit (UNFI0032)
- Product Code:
- UNFI0032
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 18.75 pg/mL
- Range:
- 31.25-2000 pg/mL
Human IL-6 ELISA Kit (HUFI00180)
- Product Code:
- HUFI00180
- Sensitivity:
- 2.813pg/ml
- Range:
- 4.688-300pg/ml
- ELISA Type:
- Sandwich
