10525 results for 'IL-5'

Molecular Biology Tools, PCR & Cloning Kits | Assay Genie

Western Blot Protocol & Troubleshooting Guide | 14 Steps + 101 Tips | Assay Genie

Ionotropic glutamate receptors (iGluRs) are pivotal in the fast excitatory synaptic transmission in the mammalian central nervous system (CNS). These receptors are not only crucial for normal brai

In Vivo Antibody Sampler Packs | Checkpoint & Depletion Panels | Assay Genie

RIPA 缓冲液配方与细胞裂解方案（中文） | Assay Genie<

GeniePlex Multiplex Assays | Bead-Based Multiplex Immunoassays | Assay Genie

免疫组织化学缓冲液与配方（IHC，中文） | Assay Genie

CD16, also known as FcγRIII (Fc gamma receptor III), plays a crucial role in the immune system, serving as a receptor for the Fc region of immunoglobulin G (IgG). This receptor is a key player in

QUICK ANSWER If your ELISA returns sample concentrations that are orders of magnitude too high, the assay itself is usually fine — the error is almost always in data analysis. The most

CD16, also known as FcγRIII (Fc gamma receptor III), plays a crucial role in the immune system, serving as a receptor for the Fc region of immunoglobulin G (IgG). This receptor is a key player in

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer ELISA and Western Blot are both antibody-based protein detection methods. ELISA is faster, higher through

Metabolism · Biochemistry Guide 10 Steps of Glycolysis: Enzymes, Diagram & ATP Yield The complete ten-step pathway that converts one glucose m

Autophagy, a fundamental cellular process, plays an indispensable role in maintaining cellular homeostasis and ensuring the efficient recycling of cellular components. Through this p

Lipid Metabolism Assay Kits | Cholesterol, Fatty Acids & Bile Acids | Assay Genie

Molecular Biology · Gene SilencingWhat is RNAi? A Complete Guide to RNA InterferenceRNA interference (RNAi) is a conserved biological process in which small double-stranded RNA molecules silence gene

Techniques · Molecular CloningGuide to Bacterial Transformation: The Science of Genetic ManipulationBacterial transformation is the process by which bacteria take up foreign DNA — usually a plasmid —

3D Organoid Cell Viability Assay (AKES081) effectively measures BENO model viability across different storage conditions. AKES081 detects significant viability differences, particularly

亚细胞分级分离方案（细胞核/线粒体/胞质，中文） | Assay Genie

蛋白质印迹缓冲液与配方（中文） | Assay Genie

Non-Small Cell Lung Carcinoma (NSCLC) is the most common type of lung cancer, representing about 85% of all lung cancer cases. NSCLC includes several subtypes, with distinct biological ch

Chemokines are small signaling proteins that play a crucial role in the immune system by directing the movement of cells, orchestrating immune responses, and maintaining tissue homeostasis. The

Lab Methods · Flow CytometryFlow Cytometry Protocol: Two Worked Protocols, Controls and 10 Practical TipsFlow cytometry measures individual cells in a fluid stream at hundreds per second, reporting si

What is preeclampsia? According to the World Health Organization (WHO), one in ten pregnant women is affected by hypertensive disorders, and preeclampsia a

Quick Facts About BevacizumabWhat is Bevacizumab?Bevacizumab is a monoclonal antibody that targets vascular endothelial growth factor (VEGF), inhibiting angiogenesis (the formation of ne

Quick Facts About TigatuzumabWhat is Tigatuzumab?Tigatuzumab is a monoclonal antibody that targets the death receptor 5 (DR5) pathway, inducing apoptosis in cancer cells.What is the mech

Nuclear Factor Erythroid 2–Related Factor 2 (NRF2) orchestrates a principal defense mechanism against oxidative stress and plays a pivotal role in inflammation and disease pathogenesis. This artic

In the realm of immunology, the terms "immunoglobulin" and "antibody" often find themselves used interchangeably, sparking confusion among those keen on understanding the immune system's fine deta

The STING (Stimulator of Interferon Genes) pathway plays a pivotal role in the innate immune system's response to cancerous cells and DNA viruses. Exploiting this pathway through STING activators

Western blotting is a powerful and widely used technique in molecular biology, essential for detecting and quantifying specific proteins in complex biological samples. However, despite its popu

SARS-CoV-2, the causative viral agent of the novel coronavirus disease COVID-19, has sparked a global effort for creating prophylactic and therapeutic interventions. The following

SfN 2024: Meet Assay Genie at the Society for NeuroscienceAssay Genie is delighted to be attending SfN 2024 in Chicago, Illinois on 5th to the 9th of October at the McCormick Convention

Cell Culture · ProtocolsCell Synchronisation Methods: Protocols for G1, S, G2 and M Phase ArrestAn asynchronous culture contains cells at every stage of the cycle at once, so any measurement is an ave

What is COVID-19 COVID-19, the disease caused by the coronavirus named SARS-CoV-2, first identified in Wuhan, China in December 2019 has spre

Hypoxia, or low oxygen levels, poses a significant challenge to cells, necessitating rapid and efficient adaptation mechanisms. The Hypoxia-Inducible Factor (HIF) pathway is a well-known regula

Immunology · Cell IsolationPeripheral Blood Mononuclear Cells (PBMCs): Composition, Isolation and UseMononuclear cells are the leukocytes with a single, unlobed nucleus — lymphocytes and monocytes. Is

By Shane Houston, PhD Candidate Queen’s University Belfast Cancer is an age old adversary of the human race. This out-of-control growth of abnormal cells has been a shadow over human health t

Ferroptosis Assay Kits | Lipid Peroxidation, Iron & Glutathione | Assay Genie

Clinical pathology labs play a crucial role in modern healthcare, providing critical diagnostic testing services for a wide array of diseases and conditions. These laboratories are design

Mesenchymal stem cells (MSCs) have emerged as pivotal players in the realm of regenerative medicine due to their unique properties and versatile potential. Originally discovered in the bone marrow

Before I even started my PhD I was already thinking about carrying out PostDoc in a foreign country and had my sights set on going to the US. I had previously carried out a degree in Genetics

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TUNEL Assay Kits | 1-Step In Situ Apoptosis Detection | Assay Genie

Sweet Deception: How a New Antibody Therapy Unmasks Pancreatic Cancer Sweet Deception: How a New Antibody Therapy Unmasks Pancreatic Cancer Pancreatic canc

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer The five main types of ELISA are sandwich, competitive, direct, indirect and multiplex. Sandwich ELISA is

細胞溶解のための超音波処理プロトコル | Assay Genie | Assay Genie

共免疫沉淀（Co-IP）方案（中文） | Assay Genie

细胞培养指南——技术与方案 | Assay Genie

The Brain's Sentinels: How Microglia Shape Neuroinflammation in Disease In the intricate landscape of the human brain, a silent, ever-watchful guardian stands ready to defend against injury and invas

Like needles in a haystack, interesting speakers that bring charisma and rock n roll to their talks are hard to come by. No matter what the research interests of the audience, these speakers hy

蛋白质印迹（Western Blot）指南（中文） | Assay Genie

Cell Culture · ProtocolsPassaging Hybridoma Cells: A Detailed GuideHybridomas are productive but temperamental. They grow in suspension, are sensitive to shear and to overgrowth, and — the problem tha

Introduction to BTLA in Immune Regulation B and T lymphocyte attenuator (BTLA) is an immune checkpoint molecule that plays a critical role in regulating immune responses by suppressing T

ELISA バッファーとレシピ | Assay Genie | Assay Genie

MORV0013 functioned effectively in both qPCR machines and water baths, with or without paraffin oil, maintaining consistent sensitivity and specificity. MORV0013 performed reliably betwe

Embryonic induced pluripotent stem cells (iPSCs) hold immense potential in the field of regenerative medicine due to their ability to differentiate into various cell types. Understanding the diffe

Objectives & Key Results Objectives & Key Results (OKRs) is a framework for setting and tracking goals. At Assay Genie, we've tailored this approach to suit researc

QUICK ANSWER A researcher ran a competitive 2,3-bisphosphoglycerate (2,3-BPG) ELISA on red blood cell (RBC) lysate. The standard curve was textbook, yet every lysate sample read at the

By Juan Quintana, PhD student, University of Edinbrugh What are Neglected Tropical Diseases (NTDs)? The World Health Organ

Metabolism · Biochemistry Guide Cellular Respiration: Stages, ATP Production & Pathway Guide How cells convert glucose and oxygen into ATP acr

Glucagon-like peptide-1 (GLP-1) is a critical hormone in the regulation of glucose metabolism and has garnered significant attention due to its therapeutic potential in managing diabetes

Apoptosis Assay Kits | Caspase, Annexin V, TUNEL & JC-1 | Assay Genie

An antibody's variable domains decide what it binds. Its Fc decides what happens next — and that is not a detail of formulation, it is half the mechanism. Which Fcγ receptor the constant region engag

Understanding the EGFR Pathway and YAP in Non-Small Cell Lung CancerThe epidermal growth factor receptor (EGFR) pathway plays a pivotal role in the development and progression of non-small cell lung c

T cell immunoglobulin and mucin-domain containing-3 (TIM-3) is an immune checkpoint receptor that plays a pivotal role in T cell exhaustion, a state where T cells lose their ability to ef

What do scientists consider their skill set when looking for jobs outside of academia? Mine where hard to sum. I was a PhD in cell biology. I could Western Blot, I had used thousands of anti

During my PhD I knew how networking was important, it helped me source reagents off other labs, get the best advice and helped me land a post doc at Cambridge University. Its not what y

Quick answer On the Periaxin (PRX) ELISA, a researcher found every serum sample reading above the top standard — optical density (OD) around 2.8–3.0 at 450 nm, well beyond the highest

Platelet adhesion is a critical process in the maintenance of hemostasis, the body's response to bleeding. This intricate process involves a series of interactions between platelets, the cellular