10341 results for 'c-Myc'

MORV0013 functioned effectively in both qPCR machines and water baths, with or without paraffin oil, maintaining consistent sensitivity and specificity. MORV0013 performed reliably betwe

As a PhD student delving deep into the realm of cellular biology, there is one antagonist that consistently challenges my research: mycoplasma. This microscopic bacterium, with its a

The MycoGenie Rapid Mycoplasma Detection Kit enables the detection of 28 Mycoplasma species in under 1 hour using a simple visual determination method, eliminating the need for expensive

The T-cell receptor is the most finely calibrated detector in immunology. It must discriminate an agonist peptide present at a few dozen copies from a self peptide present at tens of thousands, and i

Biomarkers · InflammationC-Reactive Protein (CRP): Structure, Function and Clinical UseCRP is the most widely measured marker of inflammation in medicine — fast to rise, fast to fall, and entirely non

Beginnen Sie standardmäßig mit HIER (Heat-Induced Epitope Retrieval); PIER reservieren Sie für spezifische Antigene oder Fälle, in denen HIER nachweislich versagt. Puffer-pH entscheidet dabei oft

Quick answer A researcher's samples read negative at the standard 1-hour incubation on the MycoGenie Rapid Mycoplasma Detection Kit (MORV0011), but many turned positive after 2 hours.

Mycoprotein is an alternative source of protein that can be used in place of meat. It is made from a fungi called Fusarium venenatum, and is consumed by vegetarians and vegans as a meat sub

Microbiology · AntibioticsPenicillin vs Streptomycin: The Battle of AntibioticsPenicillin and streptomycin are two of the most important antibiotics ever discovered — and they work in completely diffe

PI3K does not switch on a protein — it rewrites a membrane. Everything downstream follows from one chemical event: the 3-hydroxyl of a phosphoinositide is phosphorylated, PIP₂ becomes PIP₃, and every

Cancer Metabolism is the mechanism by which cancer cells make energy in order for them to grow and spread. Even in the presence of oxygen, cancer cells increase glucose uptake and produce lactate,

Research Antibodies: Primary, Secondary & In Vivo | Assay Genie

The inception of induced pluripotent stem cells (iPSCs) has heralded a new dawn in the realm of biomedical research and regenerative medicine, setting the stage for groundbreaking advancements in

Protocols · Western BlottingBoiling Proteins for Western BlottingHeating protein samples in SDS loading buffer denatures proteins and coats them in a uniform negative charge for clean SDS-PAGE s

Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bact

3D Organoid Cell Viability Assay (AKES081) effectively measures BENO model viability across different storage conditions. AKES081 detects significant viability differences, particularly

What is Hepatocyte Growth Factor? Hepatocyte growth factor (HGF) is a cytokine that is mainly produced by the liver. HGF is a pleiotrophic cytokine that regulates cell growth, motilit

Cryopreservation of stimulated T cells is a common technique in immunology research, allowing for long-term storage while maintaining their functional viability. Stimulated T cells—especi

Cancer Biology · Cell Signallingp53: Structure, Signalling, Regulation and Mutation in Cancerp53 is the most frequently mutated gene in human cancer, and the reason is structural: a single tetrameric

Site-Directed Mutagenesis | Protocol, Kits & Troubleshooting | Assay Genie

Cell culture techniques are fundamental to biomedical research, providing crucial insights into cellular functions, drug discovery, and disease mechanisms. A critical step in cell culture is the p

Apoptosis is not one pathway but three routes into the same execution machinery. A death ligand at the surface, a mitochondrion that loses outer-membrane integrity, and a granzyme delivered by a cyto

Cell Signalling · HaemostasisBlood Coagulation Signaling Pathways: A Critical OverviewBlood coagulation is a tightly regulated cascade in which circulating clotting factors are sequentially activated

Custom Multiplex Panels | 415+ Analytes, 5 Species | Assay Genie

Protein Analysis · ProtocolsWestern Blot Sample Preparation: A Practical GuideMost failed Western blots fail before the gel is loaded. Lysis buffer choice, inhibitor selection, accurate protein quanti

Explore antigen presentation's crucial role in adaptive immunity and its impact on cancer immunotherapy, highlighting the diversity and function of MHC class I molecules. Key Takeaway

The Interleukin-1 (IL-1) family represents a crucial group of signaling molecules that play a pivotal role in regulating the immune system and inflammatory responses. Comprising 11 members, inc

In the realm of immunotherapy, T cell expansion has emerged as a pivotal strategy, holding promise in the treatment of various diseases, particularly cancer. T cells are a critical component of

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant

Explore the complexities of Interleukin-10 (IL-10) signaling and regulation, a crucial aspect in immune response modulation and its dual roles in cancer development and therapy. Key Takeaways:

Hypoxia, or low oxygen levels, poses a significant challenge to cells, necessitating rapid and efficient adaptation mechanisms. The Hypoxia-Inducible Factor (HIF) pathway is a well-known regula

Horseshoe crabs, often referred to as "living fossils," are invaluable to modern medicine due to their unique blue blood. This blood is the source of Limulus Amebocyte Lysate (LAL), a sub

Acetylation, a chemical process characterized by the addition of an acetyl functional group to a molecule, stands as a cornerstone in both biochemical and biological landscapes. Its significanc

Th17 cells, a subset of T helper cells characterized by their production of interleukin-17 (IL-17), play a significant role in the immune system's response to pathogens and in the pathology of au

Mononuclear phagocytes, essential in immunity and homeostasis, perform diverse roles from phagocytosis to tissue repair, influencing health and disease. Key Takeaways: Mononuclear p

Articular cartilage, a key player in joint function, owes its unique properties to its extracellular matrix (ECM). This complex, highly specialized structure is not only fundamental in maintaining

Platelet activation plays a pivotal role in hemostasis, the process that stops bleeding and initiates wound healing. Central to this process is the activation of G protein-coupled receptors (GPCRs

T cells are central players in adaptive immunity, responsible for recognizing and eliminating infected or aberrant cells. Upon encountering antigens, T cells undergo clonal expansion and differ

Recent advances in immunotherapy have highlighted the importance of targeting immune checkpoints to enhance the immune system’s ability to combat cancer. A new potential target in this gr

Molecular Biology · Techniques Guide Types of PCR – 7 Methods, Principles & Applications PCR stands as a cornerstone in molecular biology, ena

In the realm of immunology, CXCL chemokines emerge as fascinating conductors. These small proteins, marked by their CXC motif, wield considerable influence over our immune responses and a ra

Transplant rejection is the fastest, most violent adaptive immune response a body can mount — and it is a response to a molecule rather than to a pathogen. Somewhere between one and ten per cent of a

Alzheimer's disease stands as a formidable challenge in the realm of neurological disorders, characterized by its progressive nature and profound impact on cognitive functions. This article delve

Western blotting, a cornerstone technique in molecular biology, biochemistry, and immunogenetics, is pivotal for detecting specific proteins in a sample. Its applications span various scientific f

The selection of a secondary antibody is a critical step in the experimental design of various immunodetection methods, including Western Blot, ELISA, immunohistochemistry (IHC), and immunofluores

Anti-angiogenic therapy does not work by starving tumours, and the trials that assumed it did are the reason the field spent a decade disappointed. Cutting off the blood supply was the original pitch

共免疫沉淀（Co-IP）方案（中文） | Assay Genie

Innate Lymphoid Cells (ILCs) represent a fascinating component of the immune system, playing a crucial role in maintaining tissue homeostasis and mounting rapid responses against pathogens. The

Western Blot Protocol & Troubleshooting Guide | 14 Steps + 101 Tips | Assay Genie

Biomarkers, measurable indicators of biological processes or responses to therapeutic interventions, play a pivotal role in modern healthcare. They provide clinicians with valuable information

For decades, the primary strategy in cancer treatment has been to trigger apoptosis, a form of programmed cell death, in malignant cells. However, many cancers develop resistance to apoptosis-inducin

Parkinson's disease (PD) is a progressive neurological disorder that primarily affects the motor system, leading to a wide array of symptoms ranging from tremors and stiffness to bradykinesia (slo

Cell Culture · ProtocolsPassaging Hybridoma Cells: A Detailed GuideHybridomas are productive but temperamental. They grow in suspension, are sensitive to shear and to overgrowth, and — the problem tha

Cell Signalling · Kinase CascadesMAPK Signaling: The Pathway of Mitogen StimulationThe mitogen-activated protein kinase (MAPK) pathway is one of the most important signalling cascades in biology, conv

Tumor-Associated Macrophages: Double Agents in the Cancer Battlefield In the intricate theater of the human body, the immune system acts as a vigilant guardian, dispatching cellular soldiers to seek