546 results for 'edit head'

Molecular Biology · ProtocolsTypical Workflow of CRISPR-Cas9 Genome EditingA CRISPR experiment is only as good as the steps around the cut. This guide walks the full workflow — designing and scoring a

Quick Facts About CetuximabWhat is Cetuximab?Cetuximab is a monoclonal antibody targeting the epidermal growth factor receptor (EGFR), widely used in cancer therapy.How does Cetuximab wor

Introduction to PD-1, PD-L1, and PD-L2 in Cancer ImmunotherapyThe PD-1/PD-L1 immune checkpoint pathway has been instrumental in cancer immunotherapy, with PD-1 (programmed death-1) inhibi

Quick Facts About MonalizumabWhat is Monalizumab?Monalizumab is a first-in-class immune checkpoint inhibitor targeting NKG2A, developed to enhance anti-tumor immune response by blocking

What You Need to Know About VopratelimabWhat is Vopratelimab?Vopratelimab is an experimental monoclonal antibody that targets the Inducible T-cell CO-Stimulator (ICOS) protein, playing a

Immunotherapy has transformed the landscape of cancer treatment by leveraging the body’s immune system to target and destroy cancer cells. A key player in this process is Inducible T-cell

Quick answer A researcher's samples read negative at the standard 1-hour incubation on the MycoGenie Rapid Mycoplasma Detection Kit (MORV0011), but many turned positive after 2 hours.

Quick Facts About EnapotamabWhat is Enapotamab?Enapotamab is an experimental antibody-drug conjugate (ADC) developed to target tumor cells selectively, offering new hope in cancer treatm

Quick Facts About DurvalumabWhat is Durvalumab?Durvalumab (Imfinzi) is a PD-L1 checkpoint inhibitor used in immunotherapy to treat various cancers, including non-small cell lung cancer (

Quick Facts About Tisotumab VedotinWhat is Tisotumab Vedotin?Tisotumab Vedotin (Tivdak™) is an antibody-drug conjugate (ADC) designed to target tissue factor (TF) and is FDA-approved for

Key Facts: PembrolizumabIs Pembrolizumab Safe?Pembrolizumab is widely recognized as safe for most patients, with side effects ranging from mild to severe. Commonly reported side effects

CD25, a key molecule in immune regulation, is integral to the function of activated lymphocytes and regulatory T cells. It's central in mediating immune responses, with implications in autoimmune

Protocols · Western BlottingBoiling Proteins for Western BlottingHeating protein samples in SDS loading buffer denatures proteins and coats them in a uniform negative charge for clean SDS-PAGE s

Announcement · Powered by Assay GenieIntroducing BioSymposia: The Gathering Place for ScienceWe’re excited to announce the launch of BioSymposia, our new sister platform and a free home for scientific

Complement is firing on your own cells right now. Not in response to anything — C3 hydrolyses spontaneously in plasma at a low, constant rate, and every hydrolysed molecule is a potential convertase

For reproducible, isoform-specific detection, choose a monoclonal antibody; for robust detection across multiple epitopes or faster turnaround, choose a polyclonal antibody. The real tradeoff is epit

Ubiquitin is a small, highly conserved protein found in eukaryotic cells that plays a crucial role in regulating various cellular processes. The process of ubiquitination involves the covalent att

Alzheimer's disease stands as a formidable challenge in the realm of neurological disorders, characterized by its progressive nature and profound impact on cognitive functions. This article delve

The advent of immune checkpoint targeting marks a significant milestone in the oncological field, offering a beacon of hope for patients battling cancer. This innovative approach leverages the bod

Quick Facts About UtomilumabWhat is Utomilumab?Utomilumab is a monoclonal antibody targeting 4-1BB (CD137), a co-stimulatory receptor on T-cells, designed to enhance anti-tumor immunity.

Key Facts About EtigilimabWhat is Etigilimab?Etigilimab is an anti-TIGIT monoclonal antibody being studied for its potential role in cancer immunotherapy.What role does Etigilimab play i

Quick Facts About AtezolizumabWhat is Atezolizumab?Atezolizumab is an anti-PD-L1 monoclonal antibody used in immunotherapy to treat various cancers by enhancing the immune system's abili

In the realm of immunology, the terms "immunoglobulin" and "antibody" often find themselves used interchangeably, sparking confusion among those keen on understanding the immune system's fine deta

Cancer immunotherapy has revolutionized treatment options, offering strategies to harness the immune system to target and destroy tumors. One emerging area of interest is the use of OX40

Quick Facts About Patritumab DeruxtecanWhat is Patritumab Deruxtecan?Patritumab Deruxtecan (HER3-DXd) is an investigational antibody-drug conjugate (ADC) targeting HER3, a receptor impli

TIGIT (T cell immunoreceptor with Ig and ITIM domains) has emerged as a promising target in cancer immunotherapy and the treatment of autoimmune diseases. As a checkpoint receptor, TIGIT

Delve into the pivotal role of Neurofilament Light chain (NEFL) in neuron structure and its emerging importance as a biomarker for various neurodegenerative diseases, offering insights into dis

Cancer immunotherapy has revolutionized the treatment landscape for various malignancies. One of the most promising therapeutic strategies is the use of immune checkpoint inhibitors, part

What if our first line of immune defense, the innate immune system, could learn from past encounters to fight future battles more effectively? For decades, we believed this was the exclusive domain of

Assay Genie attended AACR 2025 in ChicagoSean, Shane and David had a great few days in Chicago discussing research with scientists from across the globe at the Assay Genie booth. "It's g

In Vivo Methods · ProtocolsMouse Injection Guide: Intraperitoneal, Subcutaneous, Intramuscular & Intravenous RoutesRoute choice and injection technique determine how much of a dose actually reaches it

For decades, the brain has been considered an immune sanctuary, protected by the formidable blood-brain barrier. This intricate defense system, while vital for safeguarding our most complex organ, ha

Introduction to BTLA in Immune Regulation B and T lymphocyte attenuator (BTLA) is an immune checkpoint molecule that plays a critical role in regulating immune responses by suppressing T

Cure CLCN4 2025Assay Genie was honored to attend and present at the 2025 Cure CLCN4 Scientific Conference, held on May 19–20 at Friends House in London and online. This annual event, now

Every T-cell response begins with a protein being taken apart. Before a CD8 or CD4 T cell can see anything, an antigen has to be degraded into peptides of the right length, delivered to the right com

Protein Analysis · ProtocolsWestern Blot Sample Preparation: A Practical GuideMost failed Western blots fail before the gel is loaded. Lysis buffer choice, inhibitor selection, accurate protein quanti

What is Cystic Fibrosis? Cystic fibrosis (CF) is a genetic disorder that impairs the body's ability to produce sweat and thick mucus. CF causes mucus to build up in t

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The intricate process of stem cell differentiation and self-renewal is a cornerstone of developmental biology and regenerative medicine. At the heart of this complex regulatory mechanism are trans

In the evolving landscape of cellular biology, intracellular flow cytometry (ICFC) stands out as a powerful technique for analyzing the complexities within cells. This advanced method extends the

In the dynamic landscape of biomedical research, organoids have emerged as a groundbreaking tool, offering three-dimensional (3D) models that mimic the complex architecture and functionality of hu

For decades, the fight against cancer has been a relentless battle of incremental gains. While immunotherapies like CAR-T cell therapy have offered revolutionary hope, their personalized nature, high

The inception of induced pluripotent stem cells (iPSCs) has heralded a new dawn in the realm of biomedical research and regenerative medicine, setting the stage for groundbreaking advancements in

For decades, the fight against cancer has been a grueling battle of attrition, with treatments often as harsh as the disease itself. But a revolutionary approach, chimeric antigen receptor (CAR)-T cel

In the intricate world of cancer biology, a silent conversation is constantly unfolding between tumor cells and their environment. For decades, we viewed this dialogue as a one-way street, with cancer

Toll-like receptors are the reason a milligram of endotoxin can kill a mouse. They are the fastest, least discriminating layer of immunity: germline-encoded sensors that read conserved microbial chem

Techniques · Gene DeliveryTransduction vs Transfection: Understanding Gene Delivery TechniquesTransduction and transfection are the two main routes for introducing genetic material into cells. Transdu

Cancer detection often relies on advanced imaging or invasive procedures, frequently catching the disease at later, more challenging stages. Imagine a future where a simple, non-invasive test could s

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Immunology Guide MHC Class I vs Class II: Key Differences & Functions [Guide] Major Histocompatibility Complex molecules present antigens to T

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Molecular Biology · Techniques Guide Types of PCR – 7 Methods, Principles & Applications PCR stands as a cornerstone in molecular biology, ena

Immunology · Innate LymphocytesNatural Killer Cells: Receptors, Subsets and FunctionNK cells kill without prior sensitisation and without antigen-specific receptors, using a balance of activating and