10075 results for 'buffer'

RIPA (Radio Immuno Precipitation Assay) buffer is mostly used when carrying out a western blot or immunoprecipatation assay. A RIPA buffer is used in order to lyse cells and extract protein fro

免疫组织化学缓冲液与配方（IHC，中文） | Assay Genie

RIPA 缓冲液配方与细胞裂解方案（中文） | Assay Genie<

蛋白质印迹缓冲液与配方（中文） | Assay Genie

Protein Analysis · ProtocolsWestern Blot Sample Preparation: A Practical GuideMost failed Western blots fail before the gel is loaded. Lysis buffer choice, inhibitor selection, accurate protein quanti

Lab Buffers · ProtocolsPBS vs DPBS: What’s the Difference?PBS and DPBS are the everyday isotonic, pH-balanced salt solutions used to wash, dilute and transport cells and proteins. This guide bre

In the realm of biological and biochemical research, solutions play a pivotal role in various experimental procedures, from cell culture to molecular biology assays. Among the plethora o

Western Blot Protocol & Troubleshooting Guide | 14 Steps + 101 Tips | Assay Genie

Chemistry · Acid-BaseDeprotonation: Mechanism, pKa and Choosing the Right BaseDeprotonation is the removal of a proton from a molecule to give its conjugate base. Whether it happens, and how completel

Protocols & Assay Guides | ELISA, Multiplex, Western Blot | Assay Genie

Protocols · Western BlottingBoiling Proteins for Western BlottingHeating protein samples in SDS loading buffer denatures proteins and coats them in a uniform negative charge for clean SDS-PAGE s

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Both Assay Genie MORV0010 and Promega E1910 effectively quenched Firefly luciferase before Renilla detection, minimizing signal overlap. E1910 achieved 99.95% quenching efficiency, while

Western blotting is a powerful and widely used technique in molecular biology, essential for detecting and quantifying specific proteins in complex biological samples. However, despite its popu

Quick answer On the GDF-6 (Growth Differentiation Factor 6) ELISA Kit, a researcher saw an elevated blank (OD ≈ 0.41) with the two lowest standards reading at or below it, and no signa

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant

Exploring DNA's essence, this guide illuminates its structure, function, and purification techniques, essential in fields like medicine and biotechnology. Key Takeaways: DNA's Struct

Flow Cytometry · Cell SortingGuide to Fluorescence-Activated Cell Sorting (FACS)Fluorescence-activated cell sorting (FACS) is a specialised form of flow cytometry that physically isolates individual c

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Techniques · Gel ElectrophoresisAgarose vs Polyacrylamide: A Comparative AnalysisAgarose and polyacrylamide are the two workhorse gel matrices of electrophoresis. Agarose gels resolve larger DNA and R

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Structure, Function, and Detection Assays for COVID-2019 Background The global spread of the novel cor

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Testing for COVID-19 Since the start of the COVID-19 pandemic, the World Health Organisation have emphasised the crucial importance of

SARS-CoV-2 qPCR assay from Assay Genie Assay Genie have just launched a qPCR assay for the detection of SARS-CoV-2. The SARS-CoV-2 One-Step RT-qPCR Kit for Coro

BRIEF HISTORY OF THE “GREEN” EVOLUTION OF OXYGEN AND CARBON DIOXIDE WITHIN OUR ATMOSPHERE Million years ago carbon dioxide (CO2) levels used to be conside

Immunodetection · ProtocolsSecondary Antibody Incubation: Times, Conditions and TroubleshootingThe secondary antibody step determines how much signal you get and how much background comes with it. Inc

Cell Culture · ProtocolsCell Synchronisation Methods: Protocols for G1, S, G2 and M Phase ArrestAn asynchronous culture contains cells at every stage of the cycle at once, so any measurement is an ave

What does the COVID-19 Rapid POC CE-IVD Test detect? The test detects the presence of patient-generated antibodies against spike protein, the virus which causes the disease COVID-19. The tes

The Assay Genie 3D Organoid Cell Viability Assay (AKES081) provided superior results compared to ThermoScientific PrestoBlue, maintaining over 100% viability at all time points in untrea

References Key Points Three-dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fa

Research Antibodies: Primary, Secondary & In Vivo | Assay Genie

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کیت غربالگری مهار کننده آنزیم آنژیوتانسین II (ACE2) آنزیم تبدیل آنژیوتانسین II(ACE2, EC 3.4.17.23)، کربوکسی پپتیداز بخشی از سی

Quick answer On the Periaxin (PRX) ELISA, a researcher found every serum sample reading above the top standard — optical density (OD) around 2.8–3.0 at 450 nm, well beyond the highest

Enzyme-Linked Immunosorbent Assay (ELISA) is a powerful analytical biochemistry assay that utilizes antibodies and color change to identify a substance. It is widely used in the field of immunolog

Ana Catarina Ferreira Psychiatric disorders, including anxiety and depression, are highly prevalent conditions and its incidence is expected to increase as i

By Ana Catarina Ferreira, PhD Candidate, Univeristy of Minho, Portugal Psychiatric disorders, including anxiety and depression, are highly prevalent conditio

Site-Directed Mutagenesis | Protocol, Kits & Troubleshooting | Assay Genie

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Lab Methods · CentrifugationRPM to G-Force (RCF) Conversion: Formula, Calculator Method and Common ErrorsA protocol that says “spin at 3,000 rpm” is incomplete, because the force a sample experiences

Western blotting, a cornerstone technique in molecular biology, biochemistry, and immunogenetics, is pivotal for detecting specific proteins in a sample. Its applications span various scientific f

Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bact

Flow cytometry stands as a pivotal technique in the realms of molecular biology and immunology, enabling the analysis of physical and chemical characteristics of cells or particles as they flow in

Understanding the dynamics of sodium and potassium ions within biological systems is crucial for deciphering cellular processes and developing therapeutic strategies. Sodium and potassium indicato

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Immunology · Cell IsolationPeripheral Blood Mononuclear Cells (PBMCs): Composition, Isolation and UseMononuclear cells are the leukocytes with a single, unlobed nucleus — lymphocytes and monocytes. Is

Cell counting is an important part of many different scientific experiments, enabling researchers to gather crucial data about cell populations and their characteristics. In this step-by-st

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer ELISA and Western Blot are both antibody-based protein detection methods. ELISA is faster, higher through

Immunology · Cytokine SignallingTNF-alpha and Inflammation: Structure, Signalling & TherapyTumour necrosis factor alpha (TNF-α) is one of the most important pro-inflammatory cytokines in the body. Sig

3D Organoid Cell Viability Assay (AKES081) effectively measures BENO model viability across different storage conditions. AKES081 detects significant viability differences, particularly

Techniques · Molecular CloningGuide to Bacterial Transformation: The Science of Genetic ManipulationBacterial transformation is the process by which bacteria take up foreign DNA — usually a plasmid —

Millions of people are afflicted with cancer each year. 2016 statistics for the USA alone show over half a million deaths attributed to cancer 1. We have come a long way from our early underst

Descripción Para más información, por favor, contacte a hello@assaygenie.com El kit Assay Genie us

Flow Cytometry · GatingExcluding Doublets in Flow Cytometry: Pulse Geometry and Gating StrategyTwo cells passing the laser together are recorded as one event, with roughly double the signal. In DNA co

Lab Methods · Flow CytometryFlow Cytometry Protocol: Two Worked Protocols, Controls and 10 Practical TipsFlow cytometry measures individual cells in a fluid stream at hundreds per second, reporting si

Biochemistry · Cell SignallingPhosphorylation: Types, ATP Synthesis and Cell SignallingAdding a phosphate group is how cells both store energy and switch proteins on and off. The same chemistry underl

Cell Signalling · EnzymologyProtein Kinases: Function, Classification, Structure and InhibitorsProtein kinases transfer a phosphate group from ATP onto specific residues of target proteins, switching