10103 results for 'lysis buffer'

免疫组织化学缓冲液与配方（IHC，中文） | Assay Genie

RIPA 缓冲液配方与细胞裂解方案（中文） | Assay Genie<

蛋白质印迹缓冲液与配方（中文） | Assay Genie

Protein Analysis · ProtocolsWestern Blot Sample Preparation: A Practical GuideMost failed Western blots fail before the gel is loaded. Lysis buffer choice, inhibitor selection, accurate protein quanti

Chemistry · Acid-BaseDeprotonation: Mechanism, pKa and Choosing the Right BaseDeprotonation is the removal of a proton from a molecule to give its conjugate base. Whether it happens, and how completel

Lab Buffers · ProtocolsPBS vs DPBS: What’s the Difference?PBS and DPBS are the everyday isotonic, pH-balanced salt solutions used to wash, dilute and transport cells and proteins. This guide bre

Antibodies, or immunoglobulins, stand as critical components of the immune system, orchestrating the identification and neutralization of pathogens like viruses and bacteria. This extensive artic

Techniques · Gel ElectrophoresisAgarose vs Polyacrylamide: A Comparative AnalysisAgarose and polyacrylamide are the two workhorse gel matrices of electrophoresis. Agarose gels resolve larger DNA and R

Protocols & Assay Guides | ELISA, Multiplex, Western Blot | Assay Genie

Western Blot Protocol & Troubleshooting Guide | 14 Steps + 101 Tips | Assay Genie

Quick answer On the Fatty Acid Oxidation (FAO) Assay Kit (BR00001), a researcher found that a subset of skeletal-muscle samples returned negative values once the control well was subtr

Protocols · Western BlottingBoiling Proteins for Western BlottingHeating protein samples in SDS loading buffer denatures proteins and coats them in a uniform negative charge for clean SDS-PAGE s

Flow Cytometry · GatingExcluding Doublets in Flow Cytometry: Pulse Geometry and Gating StrategyTwo cells passing the laser together are recorded as one event, with roughly double the signal. In DNA co

Glycolysis & Carbohydrate Assay Kits | Glucose, Lactate, ATP | Assay Genie

Lab Methods · CentrifugationRPM to G-Force (RCF) Conversion: Formula, Calculator Method and Common ErrorsA protocol that says “spin at 3,000 rpm” is incomplete, because the force a sample experiences

Flow cytometry is a powerful analytical technology employed in cell biology, immunology, and oncology research. It enables the rapid measurement of physical and chemical characteristics of cells o

Immunophenotyping, a cornerstone of modern immunology, involves identifying and characterizing cell populations based on their surface markers. This technique provides insights into the heterog

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant

Cell-Based ELISA Kits | 1,600+ Total & Phospho Targets | Assay Genie

In the realm of biological and biochemical research, solutions play a pivotal role in various experimental procedures, from cell culture to molecular biology assays. Among the plethora o

Western blotting, a cornerstone technique in molecular biology, biochemistry, and immunogenetics, is pivotal for detecting specific proteins in a sample. Its applications span various scientific f

SuperSet DIY Development ELISA Kits | Coat Your Own Plates | Assay Genie

Western blotting is a powerful and widely used technique in molecular biology, essential for detecting and quantifying specific proteins in complex biological samples. However, despite its popu

Both Assay Genie MORV0010 and Promega E1910 effectively quenched Firefly luciferase before Renilla detection, minimizing signal overlap. E1910 achieved 99.95% quenching efficiency, while

Quick answer On the GDF-6 (Growth Differentiation Factor 6) ELISA Kit, a researcher saw an elevated blank (OD ≈ 0.41) with the two lowest standards reading at or below it, and no signa

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer ELISA and Western Blot are both antibody-based protein detection methods. ELISA is faster, higher through

Immunology · Cell IsolationPeripheral Blood Mononuclear Cells (PBMCs): Composition, Isolation and UseMononuclear cells are the leukocytes with a single, unlobed nucleus — lymphocytes and monocytes. Is

Exploring DNA's essence, this guide illuminates its structure, function, and purification techniques, essential in fields like medicine and biotechnology. Key Takeaways: DNA's Struct

Immunoglobulin antibodies stand at the forefront of the immune defense system, offering a sophisticated mechanism for recognizing and neutralizing a vast array of pathogens, including bacteria, v

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Localised Resources in Multiple Languages | Assay Genie

TUNEL Assay Kits | 1-Step In Situ Apoptosis Detection | Assay Genie

3D Organoid Cell Viability Assay (AKES081) effectively measures BENO model viability across different storage conditions. AKES081 detects significant viability differences, particularly

IHC Antibodies | Validated Antibodies for Accurate IHC Staining | Assay Genie

Immunodetection · ProtocolsSecondary Antibody Incubation: Times, Conditions and TroubleshootingThe secondary antibody step determines how much signal you get and how much background comes with it. Inc

QUICK ANSWER A researcher ran a competitive 2,3-bisphosphoglycerate (2,3-BPG) ELISA on red blood cell (RBC) lysate. The standard curve was textbook, yet every lysate sample read at the

Introduction to CD155 in Cancer ImmunotherapyCD155, also known as Poliovirus Receptor (PVR), is a transmembrane protein that plays a pivotal role in tumor immune evasion. While CD155 is n

Antibody Structure Antibody S

Introduction to SLAMF7 in Cancer Immunotherapy SLAMF7, also known as Signaling Lymphocytic Activation Molecule F7, is a surface receptor found on natural killer (NK) cells, T cells, plasm

What does the COVID-19 Rapid POC CE-IVD Test detect? The test detects the presence of patient-generated antibodies against spike protein, the virus which causes the disease COVID-19. The tes

The Assay Genie 3D Organoid Cell Viability Assay (AKES081) provided superior results compared to ThermoScientific PrestoBlue, maintaining over 100% viability at all time points in untrea

References Key Points Three-dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fa

Research Antibodies: Primary, Secondary & In Vivo | Assay Genie

Biochemistry · Cell SignallingPhosphorylation: Types, ATP Synthesis and Cell SignallingAdding a phosphate group is how cells both store energy and switch proteins on and off. The same chemistry underl

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Quick answer On the Periaxin (PRX) ELISA, a researcher found every serum sample reading above the top standard — optical density (OD) around 2.8–3.0 at 450 nm, well beyond the highest

Quick Facts About GirentuximabWhat is Girentuximab?Girentuximab is a monoclonal antibody targeting carbonic anhydrase IX (CAIX), predominantly expressed in renal cell carcinoma (RCC) cel

Quick Facts About ZolbetuximabWhat is Zolbetuximab?Zolbetuximab is a monoclonal antibody that targets Claudin 18.2, a protein overexpressed in gastric and pancreatic cancers, enhancing t

What You Need to Know About VarlilumabWhat is Varlilumab?Varlilumab is a monoclonal antibody targeting CD27, a receptor on T-cells that plays a critical role in immune modulation.What is

Enzyme-Linked Immunosorbent Assay (ELISA) is a powerful analytical biochemistry assay that utilizes antibodies and color change to identify a substance. It is widely used in the field of immunolog

Cell Biology · PathologyNecrosis: Types, Causes, Mechanisms and How It Is MeasuredNecrosis is cell death accompanied by membrane rupture and release of intracellular contents — which is what makes it

Quick Facts About LirilumabWhat is Lirilumab?Lirilumab is an anti-KIR monoclonal antibody designed to enhance natural killer (NK) cell activity by blocking killer-cell immunoglobulin-lik

Site-Directed Mutagenesis | Protocol, Kits & Troubleshooting | Assay Genie

Quick Facts About FarletuzumabWhat is Farletuzumab?Farletuzumab is a humanized monoclonal antibody targeting folate receptor alpha (FRα), a protein overexpressed in ovarian and other epi

Microbiology · AntibioticsPenicillin vs Streptomycin: The Battle of AntibioticsPenicillin and streptomycin are two of the most important antibiotics ever discovered — and they work in completely diffe

Haematology · CoagulationBlood Coagulation Pathway: Factors, Cascade and DisordersThe coagulation cascade is usually taught as two pathways converging on a common one. That framework maps neatly onto

Quick Facts About RituximabWhat is Rituximab?Rituximab is a monoclonal antibody targeting CD20, a protein found on the surface of B cells. It plays a crucial role in treating autoimmune

Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bact

Flow cytometry stands as a pivotal technique in the realms of molecular biology and immunology, enabling the analysis of physical and chemical characteristics of cells or particles as they flow in

Introduction to KIR Blockade in Cancer ImmunotherapyNatural killer (NK) cells are crucial components of the innate immune system, responsible for recognizing and destroying virally infec

Understanding the dynamics of sodium and potassium ions within biological systems is crucial for deciphering cellular processes and developing therapeutic strategies. Sodium and potassium indicato

Immunology · Complement SystemThe Alternative Complement PathwayThe alternative pathway is one of three routes that activate complement — an antibody-independent, always-on surveillance system d

Annexin V Apoptosis Assay Kits | Flow Cytometry Detection | Assay Genie

Cell counting is an important part of many different scientific experiments, enabling researchers to gather crucial data about cell populations and their characteristics. In this step-by-st

Immunology · Cytokine SignallingTNF-alpha and Inflammation: Structure, Signalling & TherapyTumour necrosis factor alpha (TNF-α) is one of the most important pro-inflammatory cytokines in the body. Sig

Biomarkers · InflammationC-Reactive Protein (CRP): Structure, Function and Clinical UseCRP is the most widely measured marker of inflammation in medicine — fast to rise, fast to fall, and entirely non