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3–5 Waschgänge: Waschpuffer im ELISA für Forschende optimieren Verwenden Sie für ELISA-Anwendungen einen speziell dafür formulierten Waschpuffer auf PBS- oder TuS-Basis mit einem milden Detergens wie

HER2 has no ligand. HER3 has no kinase. Together they are the most potent signalling pair in human biology. That single sentence explains most of what is strange about this receptor family and all of

Before I even started my PhD I was already thinking about carrying out PostDoc in a foreign country and had my sights set on going to the US. I had previously carried out a degree in Genetics

Cómo eliminar endotoxinas de proteínas recombinantes sin perder actividad biológica La forma más fiable de obtener proteínas recombinantes con niveles seguros de endotoxina es combinar medidas upstre

Over the past few years I have traversed through academia in a few different guises, first as a PhD student for about 5 years, then onto the traditional route of a post-doc, followed by a sales

Cell Signalling · MetabolismInsulin Signalling & Receptor Tyrosine KinasesInsulin controls metabolism and growth through the insulin receptor — a receptor tyrosine kinase that branches into the

Evite 3–6 meses de retrabajo: selección de anticuerpos para investigadores La selección de anticuerpos consiste en identificar y validar la molécula con la especificidad y el desempeño necesarios par

Epidermal Growth Factor (EGF) is a protein that stimulates cell growth and differentiation by binding to its receptor epidermal growth factor receptor (EGFR) which triggers ligand-induced d

Valider en labo vos anticorps pour western blot : testez 2–3 dilutions Pour un Western blot fiable, choisissez un anticorps validé sur des lysats biologiques pertinents, plutôt qu’un anticorps choisi

Flow Cytometry · Cell SortingGuide to Fluorescence-Activated Cell Sorting (FACS)Fluorescence-activated cell sorting (FACS) is a specialised form of flow cytometry that physically isolates individual c

Like needles in a haystack, interesting speakers that bring charisma and rock n roll to their talks are hard to come by. No matter what the research interests of the audience, these speakers hy

Signal 1 tells a T cell what it is looking at; signal 2 tells it whether to care. The same peptide–MHC engagement that launches a productive effector response can just as easily install an anergy pro

Making the switch! This article provides some help on CV writing tips for scientists for PhDs and PostDocs looking for jobs outside of academia and what they might need to

Flow Cytometry · GatingExcluding Doublets in Flow Cytometry: Pulse Geometry and Gating StrategyTwo cells passing the laser together are recorded as one event, with roughly double the signal. In DNA co

Metabolism · Biochemistry Guide 10 Steps of Glycolysis: Enzymes, Diagram & ATP Yield The complete ten-step pathway that converts one glucose m

Almost all of the TGF-β in a tissue is already there, and almost none of it is active. That single fact reorganises how the pathway should be studied. Unlike most cytokines, TGF-β is deposited into t

Idiopathic Pulmonary Fibrosis Idiopathic Pulmonary Fibrosis (IPF) is a chronic, irreversible and ultimately fatal disease characterized by prog

Chemistry · Acid-BaseDeprotonation: Mechanism, pKa and Choosing the Right BaseDeprotonation is the removal of a proton from a molecule to give its conjugate base. Whether it happens, and how completel

Lab Methods · Flow CytometryFlow Cytometry Protocol: Two Worked Protocols, Controls and 10 Practical TipsFlow cytometry measures individual cells in a fluid stream at hundreds per second, reporting si

I'll start this post off with a story. When I was half way through my PhD and I had found my passion for science communication, I approached my supervisor for the first time to ask “Can I ha