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QUICK ANSWER If your ELISA returns sample concentrations that are orders of magnitude too high, the assay itself is usually fine — the error is almost always in data analysis. The most

Acute pancreatitis (AP) is a condition in which activated enzymes and inflammatory mediators released from the pancreas cause damage to the pancreas and surrounding tissues. AP is furt

Gastroenterology · Barrier BiologyZonulin and Intestinal PermeabilityZonulin is the best-characterised endogenous regulator of intestinal tight junctions — the protein that opens the gut barrier on de

By Catríona Dowling, Post-Doc, RCSI I want you to come on a journey with me where we are going to the botanic gardens. You will pick a section of the garden that is yours: yours to treasure

BRIEF HISTORY OF THE “GREEN” EVOLUTION OF OXYGEN AND CARBON DIOXIDE WITHIN OUR ATMOSPHERE Million years ago carbon dioxide (CO2) levels used to be conside

Dozens of cytokines converge on four kinases and seven transcription factors. That compression is the problem JAK–STAT has to solve, and it is why this map is drawn as receptor, kinase and STAT colum

By Rachel Bleach, PhD Student, RCSI By the age of 79 years, 1 in 14 women will develop breast cancer (Global Burden of Disease Cancer 2017). Beatson reported

Molecular Biology · ProtocolsTypical Workflow of CRISPR-Cas9 Genome EditingA CRISPR experiment is only as good as the steps around the cut. This guide walks the full workflow — designing and scoring a

Oesophageal Adenocarcinoma is on the rise globally affecting more than 450,000 people worldwide (Pennathur, Gibson et al. 2013). Once diagnosed overall 5-year survival ranges from 15 % to 2

IntroductionThe human kinome is estimated to contain 518 genes, in comparison to the estimated 180 genes that comprise the phosphatome (Arena et al., 2005). These kinase genes represent a signif

Millions of people are afflicted with cancer each year. 2016 statistics for the USA alone show over half a million deaths attributed to cancer 1. We have come a long way from our early underst

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Renal Cell Carcinoma (RCC) is the most common form of kidney cancer, originating in the lining of the proximal renal tubules. RCC accounts for approximately 90% of all kidney cancers and

QUICK ANSWER A researcher ran a panel of EMT-marker ELISA kits (E-cadherin, claudin-1, N-cadherin, vimentin, ZEB1, TWIST1) on breast cell-line lysates and found E-cadherin reading "pos

Microglia Astrocytes Oligodendrocytes Glial

SARS-CoV-2, the causative viral agent of the novel coronavirus disease COVID-19, has sparked a global effort for creating prophylactic and therapeutic interventions. The following

The antigen tells a CD4 T cell to respond; the cytokine tells it what kind of response to make. A naive CD4⁺ T cell leaving the thymus is uncommitted. Within about seventy-two hours of engaging TCR/C

Immune Regulation in COVID-19: Immunomodulatory Mechanisms against SARS-CoV-2 Infection What is COVID-19?