10313 results for 'lisa i'

What are MHC Class I molecules? The MHC class I is a glycoprotein that is expressed on all nucleated cells of the human body. MHC class I are important in disease defense against viruses, ba

QUICK ANSWER If your ELISA returns sample concentrations that are orders of magnitude too high, the assay itself is usually fine — the error is almost always in data analysis. The most

RIG-I: Antiviral Properties in COVID-19 (SARS-CoV-2) Infection Key Points RIG-I is a cytosolic RNA h

Quick answer A competitive ELISA is running correctly when the blank (zero standard) gives the highest OD, the standard curve drops smoothly with rising concentration, and your samples land in the

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer The five main types of ELISA are sandwich, competitive, direct, indirect and multiplex. Sandwich ELISA is

Over the past few years I have traversed through academia in a few different guises, first as a PhD student for about 5 years, then onto the traditional route of a post-doc, followed by a sales

Enzyme-Linked Immunosorbent Assay (ELISA) is a powerful analytical biochemistry assay that utilizes antibodies and color change to identify a substance. It is widely used in the field of immunolog

QUICK ANSWER A researcher ran a competitive 2,3-bisphosphoglycerate (2,3-BPG) ELISA on red blood cell (RBC) lysate. The standard curve was textbook, yet every lysate sample read at th

Quick answer A researcher preparing a Human IL-18 ELISA wasn't sure whether the protocol's “300-fold” HRP-Streptavidin step meant adding 40 µL of concentra

Quick answer On the GDF-6 (Growth Differentiation Factor 6) ELISA Kit, a researcher saw an elevated blank (OD ≈ 0.41) with the two lowest standards reading at or below it, and no signa

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant

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Immunology Guide MHC Class I vs Class II: Key Differences & Functions [Guide] Major Histocompatibility Complex molecules present antigens to T

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Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer ELISA and Western Blot are both antibody-based protein detection methods. ELISA is faster, higher through

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By Dr. Lisa Dwane, Postdoctoral Researcher, Royal College of Surgeons in Ireland The first link between estrogen and breast cancer was made in the late 1

What do scientists consider their skill set when looking for jobs outside of academia? Mine where hard to sum. I was a PhD in cell biology. I could Western Blot, I had used thousands of anti

I'll start this post off with a story. When I was half way through my PhD and I had found my passion for science communication, I approached my supervisor for the first time to ask “Can I ha

Writer’s block can be a big stumbling block when trying to write your PhD thesis, grant or a paper you are trying to get publish. During the early stages of writing my PhD thesis I suffered

Explore antigen presentation's crucial role in adaptive immunity and its impact on cancer immunotherapy, highlighting the diversity and function of MHC class I molecules. Key Takeaway

Before I even started my PhD I was already thinking about carrying out PostDoc in a foreign country and had my sights set on going to the US. I had previously carried out a degree in Genetics

During my PhD I knew how networking was important, it helped me source reagents off other labs, get the best advice and helped me land a post doc at Cambridge University. Its not what y

Stress and anxiety are a normal part of our daily lives and impact everything we do from our relationships with loved ones to our performance in the laboratory. Like any scientist, I experienced s

Explore 29 essential tips for crafting a compelling and well-structured PhD thesis, blending effective writing practices with time management and academic rigor for a successful dissertation journ

By Dr. Niclas Thomas “Data science” is a recent addition to the vocabulary of analytics, and can mean many different things to different people. As far as

Quick Facts About AnifrolumabWhat is Anifrolumab?Anifrolumab is a monoclonal antibody that targets the type I interferon receptor, playing a crucial role in treating systemic lupus eryth

Science communication (Scicomm) is currently on the up. As rebellion against the increasing distrust of science stemmed from bigoted and ignorant leaders, scientists are turning to s

Bone Biology · BiomarkersBone Resorption: Process, Markers & Clinical SignificanceBone resorption is the process by which osteoclasts break down bone tissue and release its stored minerals into the bl

How to increase lab productivityWhen you look back at some weeks in the lab it seems like you have achieved nothing and your lab productivity can be low. Your cloning hasn’t worked or your mice

What is COVID-19 COVID-19, the disease caused by the coronavirus named SARS-CoV-2, first identified in Wuhan, China in December 2019 has spre

Immunology · T LymphocytesCD8+ T Cells: Cytotoxic Mechanisms, Memory and ExhaustionCD8+ T cells kill infected and transformed cells directly, and they do it with a specificity nothing else in the immu

By Catríona Dowling, Post-Doc, RCSI I want you to come on a journey with me where we are going to the botanic gardens. You will pick a section of the garden that is yours: yours to treasure

Alex Lloyd PhD Candidate, Dublin Institute of Technology Did you know that roughly 17.5 million people worldwide die annually from Cardiovascular diseases

Interferons (IFNs) are a group of signalling proteins that are produced and released from cells in response to viral infection and other stimuli such as normal and tumour cell survival and

TEST RAPIDO POC COVID-19 Descrizione Il kit Assay Genie solo utilizzo per la ricerca. Il kit rapido CO

Introduction to KIR Blockade in Cancer ImmunotherapyNatural killer (NK) cells are crucial components of the innate immune system, responsible for recognizing and destroying virally infec

Bone Morphogenetic Proteins (BMP) were first discovered in the 1960s by Dr.Marshall Urist, an orthopaedic surgeon at UCLA (Urist 1965). BMPs are classically associated with their roles in limb

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The STING (Stimulator of Interferon Genes) pathway plays a pivotal role in the innate immune system's response to cancerous cells and DNA viruses. Exploiting this pathway through STING activators

I have been in the situation that I’m sure many of us have. I work on genes, which if you name to any right-minded individual, sound like a random string of nonsensical gibberish. So, maybe

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Biologics · Bone MetabolismDenosumab: RANKL Inhibition, Clinical Applications and BiosimilarsDenosumab is a fully human monoclonal antibody against RANKL, the cytokine that drives osteoclast formation

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Writing a Thesis Statement Writing a thesis statement can be a frustrating task, whether you're working on your undergraduate, master's, or PhD degree. Unfortunately

Quick Facts About LitifilimabWhat is Litifilimab?Litifilimab is a monoclonal antibody developed by Biogen targeting BDCA2, a receptor on plasmacytoid dendritic cells (pDCs), to regulate

Immunology · Antigen PresentationDendritic Cells: Markers, Activation and SubtypesDendritic cells are the professional antigen-presenting cells that decide whether an immune response happens at all. T

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Immunology · Innate LymphocytesNatural Killer Cells: Receptors, Subsets and FunctionNK cells kill without prior sensitisation and without antigen-specific receptors, using a balance of activating and

PhD Interview Presentation Dos and Don’ts It is becoming increasingly common for master's and undergraduate students to present data they produced during their resea

Background I am a 3rd year PhD student in Trinity College Dublin and my project is very much an interdisciplinary one. I’m very lucky to get to work between two great labs, Dr. Aisling Dunn

The angiotensin pathway is a pivotal hormonal system that plays a crucial role in regulating blood pressure and maintaining fluid and electrolyte balance within the body. This complex biochemical

Immunology · CytokinesIFN-gamma: Signalling, Function and Roles in Inflammation, Cancer and AutoimmunityInterferon gamma is the sole type II interferon and one of the most consequential cytokines in i

Shelly Pathak PhD candidate, University of Leeds SAIDs Systemic Auto-inflammatory diseases (SAIDs) have been defined as a group of mainly inherited disorder

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The Spanish flu pandemic of 1918-1919 was the deadliest in history. It is estimated that up to 100 million people died from the virus, which is more than three times the number who d

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