10122 results for 'lysis buffer'
-
RIPA Buffer Recipe(Page)
RIPA Buffer Recipe - Cell Lysis | Assay Genie(Post)
RIPA (Radio Immuno Precipitation Assay) buffer is mostly used when carrying out a western blot or immunoprecipatation assay. A RIPA buffer is used in order to lyse cells and extract protein fro
Immunohistochemistry (IHC) Buffers & Recipes(Page) Immunohistochemical Staining, Antibodies & Buffers(Page) 6 Schritte: ELISA Blockierpuffer nach Detektionschemie wählen(Post)Der richtige Startpunkt für die meisten ELISA‑Protokolle ist Magermilchpulver (NFDM) in TBST für HRP‑basierte Assays ohne Phospho‑Ziele, typischerweise in der gängigen Konzentrationsspanne. Bei St
IHC Buffers & Recipes - Mandarin(Page)免疫组织化学缓冲液与配方(IHC,中文) | Assay Genie
RIPA Buffer Recipe - Mandarin(Page)RIPA 缓冲液配方与细胞裂解方案(中文) | Assay Genie<
Western Blot Buffers & Recipes - Mandarin(Page)蛋白质印迹缓冲液与配方(中文) | Assay Genie
Co-Immunoprecipitation (co-IP) Protocol(Page) Guide to Western Blot Sample Preparation(Post)Protein Analysis · ProtocolsWestern Blot Sample Preparation: A Practical GuideMost failed Western blots fail before the gel is loaded. Lysis buffer choice, inhibitor selection, accurate protein quanti
Deprotonation: Unveiling the Chemistry Behind It(Post)Chemistry · Acid-BaseDeprotonation: Mechanism, pKa and Choosing the Right BaseDeprotonation is the removal of a proton from a molecule to give its conjugate base. Whether it happens, and how completel
ELISA Troubleshooting: Total Protein Normalization in Lysates(Post)QUICK ANSWER A researcher ran a panel of EMT-marker ELISA kits (E-cadherin, claudin-1, N-cadherin, vimentin, ZEB1, TWIST1) on breast cell-line lysates and found E-cadherin reading "pos
What is the difference between PBS and dPBS?(Post)Lab Buffers · ProtocolsPBS vs DPBS: What’s the Difference?PBS and DPBS are the everyday isotonic, pH-balanced salt solutions used to wash, dilute and transport cells and proteins. This guide bre
Subcellular Fractionation Protocol - Mitochrondria, Nuclear, Cytosolic(Page) Comprehensive Analysis of Antibody Structure and Function(Post)Antibodies, or immunoglobulins, stand as critical components of the immune system, orchestrating the identification and neutralization of pathogens like viruses and bacteria. This extensive artic
Agarose vs Polyacrylamide: A Comparative Analysis(Post)Techniques · Gel ElectrophoresisAgarose vs Polyacrylamide: A Comparative AnalysisAgarose and polyacrylamide are the two workhorse gel matrices of electrophoresis. Agarose gels resolve larger DNA and R
Protocols(Page)Protocols & Assay Guides | ELISA, Multiplex, Western Blot | Assay Genie
FAO Activity Negative Results? Data Analysis Help(Post)Quick answer On the Fatty Acid Oxidation (FAO) Assay Kit (BR00001), a researcher found that a subset of skeletal-muscle samples returned negative values once the control well was subtr
Western Blot Protocol & Troubleshooting Guide(Page)Western Blot Protocol & Troubleshooting Guide | 14 Steps + 101 Tips | Assay Genie
Boiling Proteins for Western Blotting: Optimal Conditions and Best Practices(Post)Protocols · Western BlottingBoiling Proteins for Western BlottingHeating protein samples in SDS loading buffer denatures proteins and coats them in a uniform negative charge for clean SDS-PAGE s
Featured
Human CAT (Catalase) ELISA Kit (HUFI07676)
- Product Code:
- HUFI07676
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 9.375 pg/mL
- Range:
- 15.625-1000 pg/mL
Human MICA ELISA Kit (HUFI00015)
- Product Code:
- HUFI00015
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 46.875 pg/mL
- Range:
- 78.125-5000 pg/mL
Fatty Acid Oxidation (FAO) Assay Kit (BR00001)
- Product Code:
- BR00001
- Instrument:
- Microplate Reader
Anti-Mouse IFNAR-1 In Vivo Antibody - Low Endotoxin (IVMB0202)
- Product Code:
- IVMB0202
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- MAR1-5A3
- Protein:
- IFNAR1
- Isotype:
- Mouse IgG1
Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin (IVMB0148)
- Product Code:
- IVMB0148
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- PC61
- Protein:
- CD25
- Isotype:
- Rat IgG1 lambda
Anti-Mouse NK1.1 In Vivo Antibody - Low Endotoxin (IVMB0100)
- Product Code:
- IVMB0100
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- PK136
- Protein:
- NK1.1
- Isotype:
- Mouse IgG2a kappa
Anti-Mouse PD-1 Antibody [RMP1-14] In Vivo-Low Endotoxin (IVMB0037)
- Product Code:
- IVMB0037
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- RMP1-14
- Protein:
- PD-1
- Isotype:
- Rat IgG2a kappa
LSAMP Rabbit Polyclonal Antibody (CAB14248)
- Product Code:
- CAB14248
- Antibody Type:
- Polyclonal Antibody
MCM4 Rabbit Polyclonal Antibody (CAB13513)
- Product Code:
- CAB13513
- Antibody Type:
- Polyclonal Antibody
VEGF Rabbit Polyclonal Antibody (CAB12303)
- Product Code:
- CAB12303
- Antibody Type:
- Polyclonal Antibody
MDA ELISA Kit (UNFI0048)
- Product Code:
- UNFI0048
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 4.688 ng/mL
- Range:
- 7.813-500 ng/mL
Dopamine ELISA Kit (UNFI0013)
- Product Code:
- UNFI0013
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 0.938 ng/mL
- Range:
- 1.563-100 ng/mL
Serotonin ELISA Kit (UNFI0018)
- Product Code:
- UNFI0018
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 0.938 ng/mL
- Range:
- 1.563-100 ng/mL
PGE2 ELISA Kit (UNFI0032)
- Product Code:
- UNFI0032
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 18.75 pg/mL
- Range:
- 31.25-2000 pg/mL
Human IL-6 ELISA Kit (HUFI00180)
- Product Code:
- HUFI00180
- Sensitivity:
- 2.813pg/ml
- Range:
- 4.688-300pg/ml
- ELISA Type:
- Sandwich
Glucose Assay Kit (BA0081)
- Product Code:
- BA0081
- Instrument:
- Microplate Reader