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    The Role of Tumor Suppressor Genes in Cancer: Knudson Hypothesis & Oncogenes(Post)

    Cancer Biology · GeneticsTumour Suppressor Genes: Roles, Examples & CancerTumour suppressor genes are the brakes on cell growth — they restrain proliferation, repair damaged DNA and trigger apoptosis

    Wnt Signalling Pathway in Immunity(Post)

    What is Wnt Signalling? The Wnt signalling pathway is an evolutionarily conserved pathway that plays critical roles in the regulation of determining cell fate, polari

    Carbon Dioxide sensing in Immune Cells(Post)

    BRIEF HISTORY OF THE “GREEN” EVOLUTION OF OXYGEN AND CARBON DIOXIDE WITHIN OUR ATMOSPHERE Million years ago carbon dioxide (CO2) levels used to be conside

    Immune Cell 'Bloodhounds': Engineering Metabolite-Sensing Receptors to Infiltrate Solid Tumors(Post)

    Imagine an elite squad of soldiers sent to neutralize a hidden fortress, only to find themselves wandering aimlessly in a thick, disorienting fog just outside the gates. This is the current reality f

    Myeloid Lineage: Pioneers of Immune Cell Production(Post)

    Immunology · HaematopoiesisMyeloid Lineage: Pioneers of Immune Cell DevelopmentThe myeloid lineage is one of the two great branches of haematopoiesis, generating the innate immune and blood cells — mo

    Investigadores: ahorre días de ensayo con PBS y TBS según la técnica(Post)

    Para western blot con detección de fosforilación, use TBS o TBS-T: el fosfato de PBS puede interferir con anticuerpos anti-fosfo y con fosfatasa alcalina. Para lavados isotónicos, ELISA y cultivo

    Hemocytometer Cell Counting Procedure(Post)

    Cell Culture · ProtocolsHaemocytometer Cell Counting: Procedure, Calculation and Common ErrorsA haemocytometer turns a known chamber volume into a cell concentration, and the whole method rests on fou

    Microtubule and Mitosis review(Post)

    Introduction Microtubules are key components of the cytoskeleton and are composed of alpha and beta tubulin. These dimerise in a head to tail fashion to form 13 linear protofilaments.

    6 Schritte: ELISA Blockierpuffer nach Detektionschemie wählen(Post)

    Der richtige Startpunkt für die meisten ELISA‑Protokolle ist Magermilchpulver (NFDM) in TBST für HRP‑basierte Assays ohne Phospho‑Ziele, typischerweise in der gängigen Konzentrationsspanne. Bei St

    A Quick Guide to Myelin(Post)

    Neuroscience · Myelin BiologyA Quick Guide to MyelinMyelin is the lipid-rich insulating sheath wrapped around nerve axons. Made by oligodendrocytes in the central nervous system and Schwann cells in t

    Excluding Doublets in FACS Analysis: A Comprehensive Guide(Post)

    Flow Cytometry · GatingExcluding Doublets in Flow Cytometry: Pulse Geometry and Gating StrategyTwo cells passing the laser together are recorded as one event, with roughly double the signal. In DNA co

    Diabetic Nephropathy & Diabetic Mellitus(Post)

    Overview of Diabetes Mellitus Diabetes mellitus (DM) is a major epidemic that affects over 347 million people worldwide (Danaei et al. 2011). The World Hea

    Mechanisms of HIV persistence during HAART(Post)

    HIV Virus: Replication Cycle and Pathogenesis in absence of HAART The human immunodeficiency virus (HIV) is a formidable pathogen that has posed a significant global

    Fc‑Block verwenden: So blockieren Sie Fc‑Rezeptoren richtig(Post)

    Fc‑Block verwenden: So blockieren Sie Fc‑Rezeptoren richtig Verwenden Sie Fc‑Block, wenn Sie FcR‑reiche Zellen wie Monozyten, Makrophagen, dendritische Zellen oder B‑Zellen färben. Die Kernmaßnahme:

    His-Tag vs. Fc-Tag: Welcher Fusionstag passt zu Ihrem Protein?(Post)

    His-Tag vs. Fc-Tag: Welcher Fusionstag passt zu Ihrem Protein? His-Tag eignet sich für schnelles Screening und kosteneffiziente Aufreinigung über IMAC. Fc-Tag lohnt sich, wenn Sie Fc-eigene Eigenscha

    Innate Immunity: Components, Functions & First Line of Defence(Post)

    Immunology · Innate ImmunityInnate Immunity: Components, Functions & First Line of DefenceThe innate immune system is the body’s first line of defence — a fast, non-specific response built from physic

    Cell Synchronisation Methods(Post)

    Cell Culture · ProtocolsCell Synchronisation Methods: Protocols for G1, S, G2 and M Phase ArrestAn asynchronous culture contains cells at every stage of the cycle at once, so any measurement is an ave

    chemokine receptors and ligands(Post)

    Chemokines are highly conserved cytokines that control the movement of cells around the body. All 45 members of the chemokine family can be divided into 4 subfamilies depending on th

    TLX as a Novel Therapeutic Target(Post)

    Danka Kozareva PhD Candidate, University College Cork The generation of neurons from stem cells The process of generating

    HMGB1 in inflammation and cancer(Post)

    HMGB1 Functional Overview High mobility group box 1 ( HMGB1), a member of the high-mobility group (HMG) family, was first identified in the thymus in 1973 an