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The Essential Guide to DNA: Components, Purpose and Purification Methods(Post)
Exploring DNA's essence, this guide illuminates its structure, function, and purification techniques, essential in fields like medicine and biotechnology. Key Takeaways: DNA's Struct
Validation of MycoGenie Rapid Mycoplasma Detection Kit - A highly sensitive visual determination method for Mycoplasma detection.(Post)The MycoGenie Rapid Mycoplasma Detection Kit enables the detection of 28 Mycoplasma species in under 1 hour using a simple visual determination method, eliminating the need for expensive
Converting RPM to G-force in Centrifugation(Post)Lab Methods · CentrifugationRPM to G-Force (RCF) Conversion: Formula, Calculator Method and Common ErrorsA protocol that says “spin at 3,000 rpm” is incomplete, because the force a sample experiences
Immune Cell 'Bloodhounds': Engineering Metabolite-Sensing Receptors to Infiltrate Solid Tumors(Post)Imagine an elite squad of soldiers sent to neutralize a hidden fortress, only to find themselves wandering aimlessly in a thick, disorienting fog just outside the gates. This is the current reality f
Site Directed Mutagenesis(Page)Site-Directed Mutagenesis | Protocol, Kits & Troubleshooting | Assay Genie
CD73: Combating Tumor Immunosuppression by Targeting Adenosine(Post)Introduction to CD73 and Tumor Immunosuppression CD73, also known as ecto-5'-nucleotidase, is an enzyme found on the surface of various cells, including tumor cells and immune cells. It p
Hijacking the Skull's Gateways: A New 'Backdoor' for Brain Drug Delivery(Post)For decades, the brain has been considered an immune sanctuary, protected by the formidable blood-brain barrier. This intricate defense system, while vital for safeguarding our most complex organ, ha
Wnt Signalling Pathway in Immunity(Post)What is Wnt Signalling? The Wnt signalling pathway is an evolutionarily conserved pathway that plays critical roles in the regulation of determining cell f
Microtubule and Mitosis review(Post)Introduction Microtubules are key components of the cytoskeleton and are composed of alpha and beta tubulin. These dimerise in a head to tail fashion to form 13 linear protofilaments.
3D Cell Culture: Assay Guide(Post)References Key Points Three-dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fa
Beyond Burnout: How T Cell Exhaustion Hijacks Cancer Immunity and What We Can Do About It(Post)Imagine a highly trained soldier, elite and effective, sent to the front lines of a relentless war. Day after day, they fight without rest, their supplies dwindle, and the enemy never stops coming. Ev
Excluding Doublets in FACS Analysis: A Comprehensive Guide(Post)Flow Cytometry · GatingExcluding Doublets in Flow Cytometry: Pulse Geometry and Gating StrategyTwo cells passing the laser together are recorded as one event, with roughly double the signal. In DNA co
T Cell Types, Activation, Polarization & Function(Post)T Cell Types, Activation, Polarization & Function A comprehensive guide to T cells! Introduction T-cells, vital components of the immune system, encompass vari
CRISPR-Cas Screening for Cell Viability(Post)Key Points Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is a method of gene editing that uses t
Understanding the Autophagy Pathway: A Critical Process in Cellular Maintenance(Post)Cell Biology · Protein DegradationThe Autophagy Pathway: Mechanism, Regulation and How to Measure ItAutophagy is the cell’s route for degrading and recycling its own contents — damaged organelles, agg
BDNF in inflammation and neuropathologies(Post)BDNF Overview Brain-derived neurotrophic factor (BDNF) is a central mediator of neuroplasticity, a term used to describe the ability of neurons
Galectin Therapeutics(Post)Idiopathic Pulmonary Fibrosis Idiopathic Pulmonary Fibrosis (IPF) is a chronic, irreversible and ultimately fatal disease characterized by prog
chemokine receptors and ligands(Post)Chemokines are highly conserved cytokines that control the movement of cells around the body. All 45 members of the chemokine family can be divided into 4 subfamilies depending on th
TLX as a Novel Therapeutic Target(Post)Danka Kozareva PhD Candidate, University College Cork The generation of neurons from stem cells The process of generating
Trained Immunity: How Your Innate Immune System Learns and Remembers(Post)For decades, immunologists believed that only adaptive immunity—the sophisticated system of T cells and B cells—could form lasting memories of past infections. The innate immune system, our body's fir
T cell Immunophenotyping - A Guide(Post)Immunology · Flow CytometryT Cell Immunophenotyping: Markers, Subsets and Panel DesignImmunophenotyping identifies cell populations by the proteins on their surface. For T cells that means resolving n