Beta Actin Monoclonal Antibody

Figure 1: Western blot analysis of extracts of HeLa cells, using Beta Actin antibody (CABC026) at 1:10000-1:2560000 dilution.

Figure 2: Western blot analysis of extracts of various cell lines, using Beta Actin antibody (CABC026) at 1:100000 dilution.

What are actins?

Actins are family of highly conserved proteins that are involved in various types of cell motility, structure, and integrity. There are six different actin isoforms found in humans. These are encoded by 6 different genes: α-skeletal (expressed predominantly in striated muscle cells), α-cardiac (expressed predominantly in striated muscle cells), α-smooth (expressed predominantly in smooth muscle cells), γ-smooth (expressed predominantly in smooth muscle cells), and two ubiquitously expressed cytoplasmic (non-muscle) isoforms, β-cytoplasmic and γ-cytoplasmic. More generally, actins can be classified into 3 subgroups: alpha, beta, and gamma actins. It has been discovered that alpha actins are a major constituent of the contractile apparatus in muscle cells. Beta and gamma actins on the other hand primarily function in internal cell motility as components of the cytoskeleton.

Actin participates in many important cellular processes, including cell migration, cell division and cytokinesis, vesicle and organelle movement, cell signalling and the regulation of gene expression. Actins also help to establish and maintain cell shape and cell junctions.

Beta Actin (β-Actin)

Beta Actin is coded by the gene ACTB. It has a molecular weight of approximately 42 kDa. This protein is one of the two nonmuscle cytoskeletal actins.

Beta Actin has been shown to interact with proteins such Spectrin beta chain, brain 2 (SPTBN2), a protein demonstrated to be involved in the pathogenesis of Spinocerebellar ataxia type 5.

In addition, the mRNA encoding Beta Actin has been witnessed to interact with protein Sam68, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components.

An eight-amino acid sequence in actin has been shown to mediate the interaction between actin and eNOS. This as a result activates eNOS, thus increasing NO production.

Mutations in Beta Actin has been associated with the development in diffuse large B-Cell Lymphoma.

Figure 3: Immunofluorescence analysis of HeLa cells using ACTB antibody (CABC026) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications of Beta Actin (β-Actin)

The most common application Beta Actin is its use as a loading control in Western Blot for data normalization. Normalization of Western Blot data is an analytical step that is performed to compare the relative abundance of a specific protein across the lanes of a blot or gel under diverse experimental treatments, or across tissues or developmental stages.

The reason why this is carried out in Western Blot analysis is to control for experimental variation which introduced either via human error (e.g. inconsistent sample preparation, unequal sample loading across gel lanes, uneven protein transfer).

Why do we use Beta Actin as a loading control?

Beta Actin is one of the most common housekeeping genes used in Western Blot experiments. This is because they are thought to be expressed constitutively, at the same levels, across experiments. In this way, it can be used an an internal control to ensure experimental results are accurate and reproducible.

Why should you use the Antibody Genie Monoclonal Antibody?

Our Beta Actin (ACTB) Monoclonal Antibody (CABC026) is one of the most sensitive on the market and allows researchers to dilute the antibody 1:2,560,000 for detection by Western Blot.

At those dilution factors you'll never need to buy another Beta Actin antibody.This saves time and money, and means the focus can be on the science!

It may also be used for Immunohistochemistry, and Immunofluorescence experiments and has a wide species reactivity (Human, Mouse, Rat, Chicken, Zebrafish, Pig, Oryza sativa).

To buy our Beta Actin monoclonal antibody click here.

Figure 4: Immunohistochemistry of paraffin-embedded human breast cancer using Beta Actin antibody (CABC026) at dilution of 1:100 (20x lens).

Figure 5: Immunohistochemistry of paraffin-embedded mouse liver using Beta Actin antibody (CABC026) at dilution of 1:100 (20x lens).

Figure 6: Immunohistochemistry of paraffin-embedded rat kidney using Beta Actin antibody (CABC026) at dilution of 1:100 (20x lens).

Additional resources