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    CTLA-4 in Breast Cancer: Insights for Immunotherapy(Post)

    The Role of CTLA-4 in Breast Cancer: Implications for ImmunotherapyBreast cancer (BC) remains one of the most prevalent malignancies affecting women worldwide, with significant morbidity and mortalit ...

    Oct-4 Stem Cells(Post)

    Oct-4 stem cells, also known as Octamer-binding transcription factor 4 stem cells, are a fascinating area of study in the field of stem cell research. These stem cells hold great promise due to t ...

    Anti-CTLA-4 Immunotherapy(Post)

    CD Markers Cluster of Differentiation (CD) markers play an important role in the differentiation of B cells, T cells and NK (natural killer) c ...

    How to Thaw Cells: Best Practices for Cell Culture Success(Post)

    Cell culture techniques are fundamental to biomedical research, providing crucial insights into cellular functions, drug discovery, and disease mechanisms. A critical step in cell culture is the ...

    4-1BB: Energizing Immune Cells to Overcome Tumor Resistance(Post)

    Immunotherapy has become a central pillar in the fight against cancer, with a focus on enhancing the body’s natural defenses. Among the molecules at the forefront of immunotherapeutic re ...

    Anti-CTLA-4: Unleashing the Power of T Cells in Combination Immunotherapy(Post)

    Immunotherapy has revolutionized cancer treatment by harnessing the body's immune system to fight malignancies. Among the notable advancements in this field is the development of immune ...

    Ipilimumab and Nivolumab: Advancing Research on CTLA-4 in Cancer Therapy(Post)

    What You Need to Know About IpilimumabWhat is Ipilimumab?Ipilimumab is a monoclonal antibody that targets CTLA-4, a protein involved in downregulating the immune system, to boost the bo ...

    Boiling Proteins for Western Blotting: Optimal Conditions and Best Practices(Post)

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    Antigen Presenting Cells (APCs) and cancer immunotherapy(Post)

    Explore antigen presentation's crucial role in adaptive immunity and its impact on cancer immunotherapy, highlighting the diversity and function of MHC class I molecules. Key Takeawa ...

    VEGF: Inflammation and Tumourigenesis(Post)

    What is VEGF? Vascular Endothelial Growth Factors (VEGFs) are a group of homodimeric polypeptides, and are master regulators of vascular development, maintenance and ...

    Unraveling the Mysteries of Platelet-Activating GPCR Signaling(Post)

    Platelet activation plays a pivotal role in hemostasis, the process that stops bleeding and initiates wound healing. Central to this process is the activation of G protein-coupled receptors (GPCR ...

    BDNF in inflammation and neuropathologies(Post)

    BDNF Overview Brain-derived neurotrophic factor (BDNF) is a central mediator of neuroplasticity, a term used to describe the ability of neuron ...

    Interleukin-8 signalling(Post)

    IL-8 Overview Interleukin-8 (IL-8), also known as CXCL8, was discovered over 30 years ago as a small molecule that could promote chemotaxis and induce the production ...

    3D Organoid Cell Viability Assay: Assessing Environmental Stress on BENOs for Live Delivery(Post)

    3D Organoid Cell Viability Assay (AKES081) effectively measures BENO model viability across different storage conditions. AKES081 detects significant viability differences, particularly ...

    Wnt Signalling Pathway in Immunity(Post)

    What is Wnt Signalling? The Wnt signalling pathway is an evolutionarily conserved pathway that plays critical roles in the regulation of determining cell ...

    OKRs for Researchers(Post)

    Objectives & Key Results Objectives & Key Results (OKRs) is a framework for setting and tracking goals. At Assay Genie, we've tailored this approach to sui ...

    Lusvertikimab: Unveiling the Potential in Immuno-Oncology(Post)

    What You Need to Know About LusvertikimabWhat is Lusvertikimab?Lusvertikimab is a monoclonal antibody designed to inhibit the IL-4 receptor, a key player in regulating immune responses, ...

    CD155: Breaking Immune Suppression in Tumor Environments(Post)

    Introduction to CD155 in Cancer ImmunotherapyCD155, also known as Poliovirus Receptor (PVR), is a transmembrane protein that plays a pivotal role in tumor immune evasion. While CD155 is ...

    CD80: Amplifying Immune Activation Through Co-Stimulatory Pathways(Post)

    Introduction to CD80 and Its Role in Immune Activation CD80 is a critical immune checkpoint molecule that plays an essential role in T cell activation through co-stimulatory pathway ...

    CD86: Enhancing T Cell Activation in Immunotherapy(Post)

    Introduction to CD86 and Its Role in Immune Activation CD86 is a crucial co-stimulatory molecule that plays an essential role in T cell activation, driving the immune system’s abili ...

    Targeting Immune Checkpoints as Cancer Therapy(Post)

    In recent years, the field of oncology has witnessed a paradigm shift with the advent of immunotherapy, a treatment modality that harnesses the body's immune system to combat cancer. Among the mo ...

    OX40 Agonists: Revitalizing the Immune Response in Cancer(Post)

    Cancer immunotherapy has revolutionized treatment options, offering strategies to harness the immune system to target and destroy tumors. One emerging area of interest is the use of OX40 ...

    Utomilumab: Advancing Immuno-Oncology Research(Post)

    Quick Facts About UtomilumabWhat is Utomilumab?Utomilumab is a monoclonal antibody targeting 4-1BB (CD137), a co-stimulatory receptor on T-cells, designed to enhance anti-tumor immunity ...

    Targeting Immune Checkpoints as Cancer Therapy(Post)

    The advent of immune checkpoint targeting marks a significant milestone in the oncological field, offering a beacon of hope for patients battling cancer. This innovative approach leverages the bo ...

    Western Blot Training: A Comprehensive Guide(Post)

    Western blotting, a cornerstone technique in molecular biology, biochemistry, and immunogenetics, is pivotal for detecting specific proteins in a sample. Its applications span various scientific ...

    Auto-inflammatory diseases and genetics (SAIDs)(Post)

    Shelly Pathak PhD candidate, University of Leeds SAIDs Systemic Auto-inflammatory diseases (SAIDs) have been defined as a group of mainly inherited disorde ...

    ELISA vs Western Blot: Which Technique Should You Choose?(Post)

    Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer ELISA and Western Blot are both antibody-based protein detection methods. ELISA is f ...

    Metabolism or pluripotency – which regulates which? A chicken or the egg story.(Post)

    By Sophie Arthur How many diseases and conditions do you think exist in this world? Thousands? Hundreds of thousands? Maybe even millions? Unfortunately, I don’t know the answer. But what ...

    The Strategic Role of Secondary Antibody Incubation Times in Immunodetection Techniques(Post)

    Secondary antibodies are pivotal in the detection of target antigens in various immunodetection assays such as Western blotting, ELISA (Enzyme-Linked Immunosorbent Assay), and immunohistochemistr ...

    Dupilumab: Transforming Immunotherapy and Skin Disease Management(Post)

    What You Need to Know About DupilumabWhat is Dupilumab?Dupilumab is a fully human monoclonal antibody designed to treat various chronic inflammatory diseases by inhibiting key cytokines ...

    Advice and Guidelines from The Jack Ma Foundation for the Treatment and Prevention of COVID-19(Post)

    COVID-19 Prevention and Treatment What is COVID-19? COVID-19 is an infectious disease caused by the cor ...

    Freezing Stimulated T Cells: A Detailed Guide(Post)

    Cryopreservation of stimulated T cells is a common technique in immunology research, allowing for long-term storage while maintaining their functional viability. Stimulated T cells—espec ...

    Immune-Checkpoint Inhibition in Cancer Prevention(Post)

    Harnessing Immune-Checkpoint Inhibition for Cancer Prevention Immune-checkpoint inhibition (ICI) has emerged as a pivotal strategy in cancer therapy, particularly in the context of t ...

    Enfortumab Vedotin: Unveiling Its Role in Cancer Treatment and the Rise of Biosimilars(Post)

    Quick Facts About EnfortumabWhat is Enfortumab Vedotin?Enfortumab vedotin is an innovative cancer therapy, primarily used in treating urothelial carcinoma (bladder cancer) that has not ...

    Passaging Hybridoma Cells: A Detailed Guide(Post)

    Cell Culture · ProtocolsPassaging Hybridoma Cells: A Detailed GuideHybridomas are productive but temperamental. They grow in suspension, are sensitive to shear and to overgrowth, and — the problem th ...

    Sandwich ELISA: Step-by-Step Protocol & Troubleshooting(Post)

    Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~10 min read Quick Answer A sandwich ELISA uses two antib ...

    TNFRSF9: Enhancing Immune Cell Activity Against Tumors(Post)

    Introduction to TNFRSF9 and Its Role in Immune ActivationTNFRSF9, also known as 4-1BB or CD137, is a co-stimulatory receptor expressed on the surface of T cells, natural killer (NK) cell ...

    Types of ELISA: Direct, Indirect, Sandwich & Competitive Compared(Post)

    Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer The five main types of ELISA are sandwich, competitive, direct, indirect and multiple ...

    Ferroptosis: A New Frontier in Cancer Therapy(Post)

    For decades, the primary strategy in cancer treatment has been to trigger apoptosis, a form of programmed cell death, in malignant cells. However, many cancers develop resistance to apoptosis-induci ...

    Protease vs Peptidase: Understanding Enzymatic Digestion(Post)

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    ICOS: Stimulating the Immune System for Superior Cancer Therapy(Post)

    Immunotherapy has transformed the landscape of cancer treatment by leveraging the body’s immune system to target and destroy cancer cells. A key player in this process is Inducible T-cel ...

    The Role of Claudins in Cancer (Post)

    Introduction Claudins are a family of proteins that play a critical role in the formation of ...

    LAG-3: Revitalizing T Cells in Exhaustion for Combination Therapies(Post)

    Lymphocyte Activation Gene-3 (LAG-3) is an immune checkpoint receptor that plays a critical role in regulating T cell function. In recent years, LAG-3 has emerged as a potential target f ...

    TIGIT: A New Frontier in Cancer and Autoimmune Disease Immunotherapy(Post)

    TIGIT (T cell immunoreceptor with Ig and ITIM domains) has emerged as a promising target in cancer immunotherapy and the treatment of autoimmune diseases. As a checkpoint receptor, TIGIT ...

    Multiple Sclerosis and Stem Cells(Post)

    By Sonika Singh PhD candidate, University of Nottingham A Devastating Disease Multiple Sclerosis (MS) is a chronic and inflammatory disease ba ...

    LILT: A Novel Immune Checkpoint for Cancer Therapy(Post)

    Recent advances in immunotherapy have highlighted the importance of targeting immune checkpoints to enhance the immune system’s ability to combat cancer. A new potential target in this g ...

    10 Steps of Glycolysis: Enzymes, Diagram & ATP Yield(Post)

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    Embryonic Induced Pluripotent Stem Cell Differentiation: Pathways and Lineage-Specific Markers(Post)

    Embryonic induced pluripotent stem cells (iPSCs) hold immense potential in the field of regenerative medicine due to their ability to differentiate into various cell types. Understanding the diff ...

    Unlocking a New Immune Checkpoint: The CD47-Thrombospondin-1 Pathway in Cancer(Post)

    Cancer immunotherapy has revolutionized oncology, offering powerful new ways to treat various malignancies by harnessing the body's own immune system. Central to this success are immune checkpoint in ...

    The Rat Effectively Models Signature Cytokines of T Helper Cells(Post)

    Understanding the immune system's intricacies requires models that accurately reflect its complexity. The rat, as a model organism, plays a crucial role in immunological research, particularly i ...

    TNFR2: Taming Regulatory T Cells to Enhance Anti-Tumor Immunity(Post)

    In recent years, cancer immunotherapy has become a powerful tool for treating various malignancies. One promising approach involves targeting the tumor microenvironment, particularly reg ...

    Targeting VISTA: Unleashing the Power of T Cells in Cancer Therapy(Post)

    V-domain Ig suppressor of T cell activation (VISTA) is an emerging immune checkpoint receptor that plays a key role in suppressing T cell activity within the tumor microenvironment (TME) ...

    Transformation Protocol – Sample Transformation Protocols(Post)

    Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bac ...

    GITR: Boosting T Cell Activation for Enhanced Cancer Immunotherapy(Post)

    Glucocorticoid-induced tumor necrosis factor receptor (GITR) is an immune checkpoint molecule that plays a crucial role in regulating T cell activation and survival. In recent years, GIT ...

    CD27 Activation: Strengthening the Immune Army Against Cancer(Post)

    Harnessing the immune system to fight cancer has become a pivotal strategy in modern oncology. A key component in this fight is CD27, a receptor that plays a crucial role in T-cell activ ...

    Sensing Danger: The Biology of TLR-Mediated Inflammation(Post)

    By Eoin Mac Réamoinn Toll-like receptors (TLRs) are renowned for their fundamental roles in innate sensing and initiating inflammatory responses. TLR ...

    Beyond Burnout: How T Cell Exhaustion Hijacks Cancer Immunity and What We Can Do About It(Post)

    Imagine a highly trained soldier, elite and effective, sent to the front lines of a relentless war. Day after day, they fight without rest, their supplies dwindle, and the enemy never stops coming. E ...

    Toll-Like Receptor Signaling Pathways: A Key to Innate Immunity(Post)

    In the intricate landscape of the immune system, toll-like receptors (TLRs) play a fundamental role in the first line of defense against pathogens. These receptors, essential components of the in ...

    Physiological Roles for IL-33 | Assay Genie(Post)

    By Charlotte O'Donnell PhD A role for IL-33 in Barrier Function and Epithelial Wound Healing ...

    Toll-like Receptor Signalling in Neurodegenerative Disease(Post)

    Toll-Like Receptors The innate immune response has come under the spotlight in recent years due to its central role in propagating the pathoge ...

    Understanding T Cell Fatigue: Implications for Immune Function and Therapeutic Interventions(Post)

    T cells are central players in adaptive immunity, responsible for recognizing and eliminating infected or aberrant cells. Upon encountering antigens, T cells undergo clonal expansion and diffe ...

    8 Steps to Mindfulness in the Laboratory(Post)

    Stress and anxiety are a normal part of our daily lives and impact everything we do from our relationships with loved ones to our performance in the laboratory. Like any scientist, I experienced ...

    인터루킨 8 시그널링(Page)

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    Custom ELISA Kits(Page)

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    Human Multiplex Inflammation ELISA Panels(Page)

    The ELISA Genie, Human Inflammation multiplex assays utilizes multiple bead populations differentiated by size and different levels of fluorescence intensity. With multiple sizes of bea ...

    Human Multiplex ELISA Panels(Page)

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    Mouse Multiplex ELISA Panels(Page)

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    Validated GeniePlex Panels(Page)

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    GeniePlex Validated Panels by Cytometer(Page)

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    Validated Multiplex Panels by Flow Cytometer(Page)

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    Distributors - Bahrain(Page)

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    Distributors - United Arab Emirates(Page)

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    Validated GeniePlex Panels - Customer Applications(Page)

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    CAR-T cell signalling(Page)

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    Regulatory T-cell (Treg) suppression & the IL-2 axis(Page)

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    Distributors - Oman(Page)

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    Adaptive immune system molecules(Page)

    Co-stimulatory and Co-inhibitory Molecules in Adaptive Immunity The adaptive immune system is in part regulated by a vast array of co-stim ...

    RIPA Buffer Recipe(Page)

    What is RIPA Buffer? RIPA (Radio Immuno Precipitation Assay) buffer is primarily used when conducting a western blot or immunoprecipatation assay. A RIPA buffer is used in order to l ...

    Co-Immunoprecipitation (co-IP) Protocol(Page)

    Co-immunoprecipitation Protocol A detailed guide of how Co-IP works, a step by step Co-IP protocol and some hints & tips for your Co-IP workflow! Introductio ...

    IHC Buffers & Recipes - Mandarin(Page)

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    B-cell activation and the germinal centre reaction(Page)

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    CD4+ T-helper cell differentiation(Page)

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    T-cell receptor (TCR) signalling(Page)

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    ELISpot Assay Kits(Page)

    ELISpot 400x more sensitive than ELISA! Detection & Visualisation at the Single-Cell Level Explore ELISpot ...

    Custom Antibody(Page)

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    Interactive Pathway Diagrams(Page)

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    Chemokines(Page)

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    Western Blot Protocol & Troubleshooting Guide(Page)

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    Immunohistochemical Staining, Antibodies & Buffers(Page)

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    Cytokine neutralisation network(Page)

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    Subcellular Fractionation Protocol - Mitochrondria, Nuclear, Cytosolic(Page)

    What is Subcellular Fractionation? Subcellular fractionation is the process of iIsolating nuclear, cytosolic and mitochondrial fractions of high purity from tissues. Subcellular fracti ...

    T-Cell Co-Stimulation & Agonist Checkpoint Pathway(Page)

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    Multiplex Assays(Page)

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    Coronavirus Assays(Page)

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    Cytokine JAK/STAT signalling(Page)

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