Acetyl-CoA Fluorometric Assay Kit (BN00584)

(No reviews yet) Write a Review
SKU:
BN00584
  • Acetyl-CoA Fluorometric Assay Kit (BN00584)
  • Acetyl-CoA Fluorometric Assay Kit (BN00584)
£580.76

Description

ELISA Kit Technical ManualMSDS

Acetyl-CoA Fluorometric Assay Kit

Acetyl CoA is a central molecule of metabolism. It carries acetate, used in the build-up and breakdown of larger molecules. Acetyl CoA is key in synthetic pathways leading to sesquiterpenes, precursors to cholesterol and other sterols, flavenoids and other polyketides, polyenes and long-chain fatty acids. It is the source of the acetyl group used in histone acetylation. The acetyl group is also incorporated into a variety of other molecules such as acetylcholine, melatonin, heme and TCA cycle intermediates. Assay Genie has developed a highly sensitive assay for determining Acetyl CoA level in a variety of biological samples. In the assay, free CoA is quenched then Acetyl CoA is converted to CoA. The CoA is reacted to form NADH which interacts with PicoProbe to generate fluorescence (Ex=535/Em=587 nm). The assay can detect 10 to 1000 pmol of Acetyl CoA (with detection limit ~0.4 µM) in a variety of samples.

Figure 1: Standard Curves were generated following the Kit Protocol.

Key Information Description

Product SKU

BN00584

Size

100 assays

Detection Methods

Fluorescence (Ex/Em 535/587 nm)

Species Reactivity

Mammalian

Applications

The assay can detect 10 to 1000 pmol of Acetyl CoA (with detection limit ~0.4 ) in a variety of samples.

Features and Benefits

  • Very simple procedure
  • Convenient
  • Assay Genie has developed a high sensitivity assay for Acetyl CoA level in a variety of biological samples. In the assay, free CoA is quenched then Acetyl CoA is converted to CoA. The CoA is reacted to form NADH which interacts with PicoProbe to generate fluorescence

Kit Components

  • Acetyl CoA Assay Buffer
  • PicoProbe
  • Conversion Enzyme
  • Acetyl CoA Enzyme Mix
  • Acetyl CoA Substrate Mix
  • CoA Quencher
  • Quench Remover
  • Acetyl CoA Standard (10 µmol)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose