Calcein AM Cell Viability Assay Kit (Fluorometric) (BN00563)

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SKU:
BN00563
£445.66

Description

ELISA Kit Technical ManualMSDS

Calcein AM Cell Viability Assay Kit

Quantification of number of viable cells is an indispensible tool in Cell Biology research. Assay Genie's Calcein AM Cell Viability Assay Kit is a fluorometric method for extremely sensitive quantification of viable cells that can detect as low as 50 viable cells in less than 30 min.

Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. Hydrolysis of Calcein AM by intracellular esterase produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.

Figure 1: Cell Viability Assay: Fibroblast cells were grown in DMEM supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were serially diluted in a clear cell culture plate and incubated for 30 min. with Calcein AM at 37°C. After incubation, cells were lysed using Cell Lysis Buffer for 10 min at room temperature. Cell lysates were transferred into a 96-well white plate & fluorescence was measured. Inset graph is an expanded segment of the assay data at lower cell number per well.

Key Information Description

Product SKU

BN00563

Size

1000 assays

Detection Method

Fluorescence (Ex/Em = 485/530 nm)

Application

  • Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients.
  • Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals.
  • Assessment of physiological mediators and antibodies that affect cell growth.

Features & Benefits

  • Convenient & non-radioactive
  • High-throughput Highly-sensitive: detect as low as 50 viable cells in less than 30 min.
  • Suitable for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis

Kit Components

  • Calcein AM
  • Calcein Dilution Buffer

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

Usage

For Research Use Only! Not For Use in Humans.

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