Peroxidase Activity Colorimetric/Fluorometric Assay Kit (BN00985)

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SKU:
BN00985
  • Peroxidase Activity Colorimetric/Fluorometric Assay Kit (BN00985)
  • Peroxidase Activity Colorimetric/Fluorometric Assay Kit (BN00985)
£478.50

Description

ELISA Kit Technical ManualMSDS

Peroxidase Activity Colorimetric/Fluorometric Assay Kit

Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues. Assay Genie's Peroxidase Assay Kit provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the GenieRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (lmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.

Figure: Colorimetric/Fluorometric H2O2 standard Curves obtained using kit protocol.

Key Information Description

Product SKU

BN00985

Size

100 assays

Detection Method

Absorbance (570 nm) or Fluorescence (Ex/Em=535/587)

Species Reactivity

Mammalian

Applications

The kit has a detection limit of 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.

Features and Benefits

The assay is simple, direct, highly sensitive and high-throughput adaptable

Kit Components

  • Assay Buffer
  • GenieRed Probe (in DMSO)
  • HSubstrate (0.88 M)
  • HRP Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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