5094 results for 'back print'

Un plan expérimental ELISA fiable repose sur six éléments non négociables: des contrôles complets (blanc, négatif, positif, matrice), des étalons pour la courbe de calibration, une série de diluti

By Dr. Niclas Thomas “Data science” is a recent addition to the vocabulary of analytics, and can mean many different things to different people. As far as

I'll start this post off with a story. When I was half way through my PhD and I had found my passion for science communication, I approached my supervisor for the first time to ask “Can I ha

Techniques · Molecular CloningGuide to Bacterial Transformation: The Science of Genetic ManipulationBacterial transformation is the process by which bacteria take up foreign DNA — usually a plasmid —

Cell Signalling · Kinase CascadesMAPK Signaling: The Pathway of Mitogen StimulationThe mitogen-activated protein kinase (MAPK) pathway is one of the most important signalling cascades in biology, conv

For decades, the battle against cancer has focused on the cellular 'soldiers' and the genetic 'blueprints' within them. Yet, a vast, invisible world of communication exists between these cells—a secr

Delve into the pivotal role of Neurofilament Light chain (NEFL) in neuron structure and its emerging importance as a biomarker for various neurodegenerative diseases, offering insights into dis

Microbiology · AntibioticsPenicillin vs Streptomycin: The Battle of AntibioticsPenicillin and streptomycin are two of the most important antibiotics ever discovered — and they work in completely diffe

The antigen tells a CD4 T cell to respond; the cytokine tells it what kind of response to make. A naive CD4⁺ T cell leaving the thymus is uncommitted. Within about seventy-two hours of engaging TCR/C

QUICK ANSWER A researcher ran a panel of EMT-marker ELISA kits (E-cadherin, claudin-1, N-cadherin, vimentin, ZEB1, TWIST1) on breast cell-line lysates and found E-cadherin reading "pos

Techniques · Apoptosis DetectionTUNEL Staining: The Method of Choice for Measuring Cell DeathTUNEL staining is a gold-standard technique for detecting apoptosis in situ. By labelling the fragmented DN

Quick answer A researcher measuring dopamine in mouse brain tissue got no detectable signal and, with many samples to run, asked whether the full standard curve could be skipped to sav

What is a vaccine? A vaccine is a pharmacological product that gives the recieving patient's immune system a chance to learn how to fight infection with decreased risk of illness. Vacci

Enzymology · Protein DegradationProtease vs Peptidase: Understanding the DifferenceProteases and peptidases are the enzymes that cleave peptide bonds — breaking proteins down into peptides and peptide

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Techniques · Gene DeliveryTransduction vs Transfection: Understanding Gene Delivery TechniquesTransduction and transfection are the two main routes for introducing genetic material into cells. Transdu

What if our first line of immune defense, the innate immune system, could learn from past encounters to fight future battles more effectively? For decades, we believed this was the exclusive domain of

By Shane Houston, PhD Candidate Queen’s University Belfast Cancer is an age old adversary of the human race. This out-of-control growth of abnormal cells has been a shadow over human health t

A depletion experiment is only as good as the depletion you can prove. Injecting a functional-grade antibody and assuming the population is gone is the most common failure mode in in vivo immunology.

Validate Western Blot Antibodies: Datasheet Checks Labs Should Ask Reliable results come from antibodies validated specifically for denatured western blot conditions: recombinant monoclonals or well-

Imagine an elite squad of soldiers sent to neutralize a hidden fortress, only to find themselves wandering aimlessly in a thick, disorienting fog just outside the gates. This is the current reality f

Metabolism · Biochemistry GuideBeta-Oxidation of Fatty AcidsBeta-oxidation is the mitochondrial pathway that breaks fatty acids down into acetyl-CoA for energy. This guide covers where it happen

Science communication (Scicomm) is currently on the up. As rebellion against the increasing distrust of science stemmed from bigoted and ignorant leaders, scientists are turning to s

Immunology Guide MHC Class I vs Class II: Key Differences & Functions [Guide] Major Histocompatibility Complex molecules present antigens to T

Pancreatic cancer survival rates Despite recent advances in our understanding of this disease, pancreatic cancer is one of the world’s most lethal malignan

Cancer Biology · GeneticsTumour Suppressor Genes: Roles, Examples & CancerTumour suppressor genes are the brakes on cell growth — they restrain proliferation, repair damaged DNA and trigger apoptosis

Imagine a highly trained soldier, elite and effective, sent to the front lines of a relentless war. Day after day, they fight without rest, their supplies dwindle, and the enemy never stops coming. Ev

Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bact

You are only part human. In some ways, you are as much bacteria as you are mammal, and in some ways bacteria make up far more of you than your own familiar cells and genes! It is becoming

By Francisco Algaba Chueco In this post we discuss a topic that everyone will have heard of. Let’s talk about migraines, a disorder that hides a huge physiol

T Cell Types, Activation, Polarization & Function A comprehensive guide to T cells! Introduction T-cells, vital components of the immune system, encompass vari

by Ryan Wallis, PhD Aging and Disease Imagine a world where everything you do is missing just a little piece and as time goes on you start to lose sense entirely. This is the deva

Cell Biology · Protein DegradationThe Autophagy Pathway: Mechanism, Regulation and How to Measure ItAutophagy is the cell’s route for degrading and recycling its own contents — damaged organelles, agg

Chemokines are highly conserved cytokines that control the movement of cells around the body. All 45 members of the chemokine family can be divided into 4 subfamilies depending on th

Explore antigen presentation's crucial role in adaptive immunity and its impact on cancer immunotherapy, highlighting the diversity and function of MHC class I molecules. Key Takeaway

Cell Death · BiomarkersCleaved Caspase-3: Activation, Function and Detection in ApoptosisCaspase-3 sits at the point where apoptotic signalling becomes irreversible. It is made as an inactive proenzym

For decades, immunologists believed that only adaptive immunity—the sophisticated system of T cells and B cells—could form lasting memories of past infections. The innate immune system, our body's fir