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Types of ELISA: Direct, Indirect, Sandwich & Competitive Compared(Post)
Written by Seán Mac Fhearraigh, PhD • Updated: 19 May 2026 • ~9 min read Quick Answer The five main types of ELISA are sandwich, competitive, direct, indirect and multiplex. Sandwich ELISA is
FAO Activity Negative Results? Data Analysis Help(Post)Quick answer On the Fatty Acid Oxidation (FAO) Assay Kit (BR00001), a researcher found that a subset of skeletal-muscle samples returned negative values once the control well was subtr
Sample Dilution Range Calculation for Competitive ELISA kits(Post)Quick answer A competitive ELISA is running correctly when the blank (zero standard) gives the highest OD, the standard curve drops smoothly with rising concentration, and your samples land in the
Ferroptosis Assays(Page)Ferroptosis Assay Kits | Lipid Peroxidation, Iron & Glutathione | Assay Genie
Lipid Metabolism Assays(Page)Lipid Metabolism Assay Kits | Cholesterol, Fatty Acids & Bile Acids | Assay Genie
LPS ELISA Troubleshooting: The Importance of Sample Nature(Post)Quick answer A researcher measuring circulating lipopolysaccharide (LPS) in human serum with our LPS ELISA saw almost no color, even after 30 minutes with substrate. Samples had been test
ELISA Cross-Reactivity: Tagged Standards vs Native Samples(Post)Quick answer A researcher validating two isoform assays — the Human ALT1 (HUFI00557) and ALT2 (HUFI00593) ELISA kits — spiked the ALT2 kit's standard onto the ALT1 kit's plate and saw
Oxidative Stress Assays(Page)Oxidative Stress Assay Kits | ROS, Antioxidant & Glutathione | Assay Genie
Immunoassays(Page)Immunoassays: ELISA, Multiplex & ELISpot Kits | Assay Genie
Transformation Protocol – Sample Transformation Protocols(Post)Introduction Transformation of bacteria with plasmids is not only of note for bacterial studies but also can be used for gene expression studies in mammalian cells. Most plasmids are of bact
Whitepapers(Page) Glycolysis & Carbohydrates Assay Kits(Page)Glycolysis & Carbohydrate Assay Kits | Glucose, Lactate, ATP | Assay Genie
ELISA vs Western Blot: Which Technique Should You Choose?(Post)Written by Seán Mac Fhearraigh, PhD • Updated: 19 May 2026 • ~9 min read Quick Answer ELISA and Western Blot are both antibody-based protein detection methods. ELISA is faster, higher through
Guide to Fluorescence Activated Cell Sorting (FACs): The Latest Technology in Cell Separation(Post)Flow Cytometry · Cell SortingGuide to Fluorescence-Activated Cell Sorting (FACS)Fluorescence-activated cell sorting (FACS) is a specialised form of flow cytometry that physically isolates individual c
Urea Assays(Page)Urea Assay Kits | Urea Cycle, Ammonia & Amino Acid Assays | Assay Genie
Hemocytometer Cell Counting Procedure(Post)Cell Culture · ProtocolsHaemocytometer Cell Counting: Procedure, Calculation and Common ErrorsA haemocytometer turns a known chamber volume into a cell concentration, and the whole method rests on fou
Apoptosis Assays(Page)Apoptosis Assay Kits | Caspase, Annexin V, TUNEL & JC-1 | Assay Genie
Competitive vs Sandwich ELISA: Should You Subtract the Blank?(Post)Quick answer: A researcher subtracted the blank from a competitive ELISA (a Serotonin kit) and every result turned negative. In a competitive assay the signal is inversely proportional to
QuickStep ELISA Kits(Page)QuickStep ELISA Kits | 90-Minute 1-Wash Assays | Assay Genie
Mouse Multiplex Inflammation ELISA Panels(Page)Featured
Human CAT (Catalase) ELISA Kit (HUFI07676)
- Product Code:
- HUFI07676
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 9.375 pg/mL
- Range:
- 15.625-1000 pg/mL
Human MICA ELISA Kit (HUFI00015)
- Product Code:
- HUFI00015
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 46.875 pg/mL
- Range:
- 78.125-5000 pg/mL
Fatty Acid Oxidation (FAO) Assay Kit (BR00001)
- Product Code:
- BR00001
- Instrument:
- Microplate Reader
Anti-Mouse IFNAR-1 In Vivo Antibody - Low Endotoxin (IVMB0202)
- Product Code:
- IVMB0202
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- MAR1-5A3
- Protein:
- IFNAR1
- Isotype:
- Mouse IgG1
Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin (IVMB0148)
- Product Code:
- IVMB0148
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- PC61
- Protein:
- CD25
- Isotype:
- Rat IgG1 lambda
Anti-Mouse NK1.1 In Vivo Antibody - Low Endotoxin (IVMB0100)
- Product Code:
- IVMB0100
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- PK136
- Protein:
- NK1.1
- Isotype:
- Mouse IgG2a kappa
![Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214372/569320/anti-mouse-pd-1-cd279-rmp1-14-in-vivo-antibody-low-endotoxin__18202.1743527968.jpg?c=2 "Anti-Mouse PD-1 (CD279) [RMP1-14] In Vivo Antibody - Low Endotoxin")
Anti-Mouse PD-1 Antibody [RMP1-14] In Vivo-Low Endotoxin (IVMB0037)
- Product Code:
- IVMB0037
- Antibody Type:
- Functional-Grade In Vivo Antibody
- Clone:
- RMP1-14
- Protein:
- PD-1
- Isotype:
- Rat IgG2a kappa
LSAMP Rabbit Polyclonal Antibody (CAB14248)
- Product Code:
- CAB14248
- Antibody Type:
- Polyclonal Antibody
MCM4 Rabbit Polyclonal Antibody (CAB13513)
- Product Code:
- CAB13513
- Antibody Type:
- Polyclonal Antibody
VEGF Rabbit Polyclonal Antibody (CAB12303)
- Product Code:
- CAB12303
- Antibody Type:
- Polyclonal Antibody
MDA ELISA Kit (UNFI0048)
- Product Code:
- UNFI0048
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 4.688 ng/mL
- Range:
- 7.813-500 ng/mL
Dopamine ELISA Kit (UNFI0013)
- Product Code:
- UNFI0013
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 0.938 ng/mL
- Range:
- 1.563-100 ng/mL
Serotonin ELISA Kit (UNFI0018)
- Product Code:
- UNFI0018
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 0.938 ng/mL
- Range:
- 1.563-100 ng/mL
PGE2 ELISA Kit (UNFI0032)
- Product Code:
- UNFI0032
- Detection Method:
- Competitive ELISA, Coated with Antigen
- Sensitivity:
- 18.75 pg/mL
- Range:
- 31.25-2000 pg/mL
Human IL-6 ELISA Kit (HUFI00180)
- Product Code:
- HUFI00180
- Sensitivity:
- 2.813pg/ml
- Range:
- 4.688-300pg/ml
- ELISA Type:
- Sandwich
