TYL (Tylosin) ELISA Kit (FSES0062)

SKU:
FSES0062
Product type:
ELISA
Application:
ELISA
€394

Description

Technical Manual

TYL (Tylosin) ELISA Kit

Kit Name: TYL (Tylosin) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Tylosin (TYL) in samples, such as urine, raw milk, eggs, tissues, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate,antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, TYL in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-TYL antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of TYL. The concentration of TYL in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 1 ppb (ng/mL)
Reaction Mode: 25°C, 15 min~15min~15min
Detection Limit: Tissue ---5 ppb; Urine ---10 ppb; Egg, Raw milk---20 ppb.
Cross Reactivity:

Tylosin

100%

Tilmicosin

1%

Erythromycin

<0.1%

Eprinomectin

<0.1%

Doramectin

<0.1%

Ivermectin

<0.1%

Avermectin

<0.1%

Emamectin

<0.1%

Moxidectin

<0.1%

Sample Recovery Rate: 90%±30%.
Kits Components:
Item Specifications
ELISA Microplate 96 wells
Standard Liquid 1.5 mL each (0 ppb, 1 ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb)
20×Concentrated Sample Solution 5 mL
HRP Conjugate 12 mL
Antibody Working Solution 7 mL
Substrate Reagent A 7 mL
Substrate Reagent B 7 mL
Stop Solution 7 mL
20×Concentrated Wash Buffer 25 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate reader,Oscillators, Centrifuge, Balance (sensibility 0.01g),
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: Methanol.
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.
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