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15d-PGJ2 (15-Deoxy-Delta12,14-prostaglandin J2) ELISA Kit (UNES00055) (UNES00055)

SKU:
UNES00055
Product Type:
ELISA Kit
Size:
96 Assays
Sensitivity:
1.88ng/mL
Range:
3.13-200ng/mL
ELISA Type:
Competitive
Reactivity:
Universal
Sample Type:
Serum, plasma and other biological fluids
€599
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Description

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Product Name:15d-PGJ2 (15-Deoxy-Delta12,14-prostaglandin J2) ELISA Kit (UNES00055)
SKU:UNES00055
Target:15d-PGJ2 (15-Deoxy-Delta12,14-prostaglandin J2) ELISA Kit (UNES00055)
Size:96T
Assay type:Competitive-ELISA
Assay time:2.0h
Sensitivity:1.88 ng/mL
Detection range:3.13-200 ng/mL
Kit component:
ComponentQuantity (24 Assays)Quantity (96 Assays)Storage
Micro ELISA Plate (Dismountable)8 wells x 3 strips8 wells x 12 strips-20°C, 6 months
Reference Standard1 vial2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 60 µL1 vial, 120 µL
Concentrated HRP Conjugate (100×)1 vial, 60 µL1 vial, 120 µL-20°C (shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL1 vial, 20 mL4°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL1 vial, 30 mL
Substrate Reagent1 vial, 10 mL1 vial, 10 mL4°C (shading light)
Stop Solution1 vial, 10 mL1 vial, 10 mL4°C
Plate Sealer5 pieces5 pieces
Product Description1 copy1 copy

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 15d-PGJ2. During the reaction, 15d-PGJ2 in the sample or standard competes with a fixed amount of 15d-PGJ2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 15d-PGJ2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 15d-PGJ2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Linearity:
Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)92-10789-10096-108
Average (%)10095103
1:4Range (%)97-11391-10590-106
Average (%)1049897
1:8Range (%)101-11488-10286-98
Average (%)1079492
1:16Range (%)95-11090-10387-100
Average (%)1019694
Recovery:
Sample TypeRange (%)Average Recovery (%)
Serum (n=5)92-10699
EDTA plasma (n=5)94-10799
Cell culture media (n=5)93-106100
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n2020.020.020.020.020.0
Mean (ng/mL)422851.52917.8453.9842.53029.8
Standard deviation28.340.0116.726.843.0160.6
C V (%)6.714.74.05.95.15.3
Sample type &Sample volume:Serum, plasma and other biological fluids; 50μL
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of 15d-PGJ2 concentrations in Serum, plasma and other biological fluids.
Specificity:This kit recognizes 15d-PGJ2 in samples. No significant cross-reactivity or interference between 15d-PGJ2 and analogues was observed.

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