The Lambda Light chain Monoclonal Antibody (CAB19831) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in a variety of applications, including Western blotting.The lambda light chain is an essential component of immunoglobulins and plays a crucial role in the immune response. By targeting the lambda light chain, researchers can gain valuable insights into antibody function and production, making this antibody an invaluable tool for studies in immunology and oncology.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Lambda Light chain Monoclonal Antibody
SKU:
CAB19831
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2357
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
V1-19, IGLV151, Lambda Light chain
Positive Sample:
Mouse spleen, Rat spleen
Cellular Localization:
Secreted, Cell Membrane.
Calculated MW:
12kDa
Observed MW:
25kDa
Predicted to be involved in immune response. Located in extracellular exosome.
Purification Method
Affinity purification
Gene ID
28820
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse spleen, using Lambda Light chain Rabbit mAb (CAB19831) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from Rat spleen, using Lambda Light chain Rabbit mAb (CAB19831) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Lambda Light chain Rabbit mAb (CAB19831) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
