Description
Anti-Mouse CD115 (CSF-1R) In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse CD115 (CSF-1R) In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD115 (CSF-1R) protein, crucial for macrophage and monocyte signaling, making it ideal for research in immunology, oncology, and related fields. With a rat IgG2a isotype, it ensures ultra-low endotoxin levels (<0.1 EU/μg), perfect for a range of assays including ELISA, flow cytometry, and functional studies. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Elevate your research with this reliable and versatile antibody. CD115, also known as colony-stimulating factor-1 receptor (CSF-1R), is a protein expressed on the surface of myeloid lineage cells, including monocytes, macrophages, and osteoclasts. It plays a pivotal role in the survival, proliferation, and differentiation of these cells by binding to its ligands, CSF-1 and IL-34, and triggering downstream signaling pathways. This makes CD115 a key player in immune regulation and a target of interest in various disease models.
| Product Name: | Anti-Mouse CD115 (CSF-1R) In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0002 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | AFS98 |
| Protein: | CD115 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Macrophage Colony Stimulating Factor Receptor, M-CSFR, CSF-R1, C-fms, CSF-1R |
| Isotype: | Rat IgG2a κ |
| Reactivity: | Mouse |
| Immunogen: | Not available |
| Applications: | B, CyTOF®, Depletion, FA, FC |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CyTOF®, Depletion, FA, FC |
| Recommended Usage: | FC The suggested concentration for this AFS98 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone AFS98 recognizes an epitope on mouse CD115. |
| Antigen Distribution: | CD115 is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid cells, dendritic cells, and osteoclasts. |
| Immunogen: | Not available |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD115 antibody, clone AFS98, recognizes CD115, also known as mouse colony-stimulating factor 1 receptor (CSF-1R and macrophage colony-stimulating factor receptor (M-CSFR). CD115 is a 150kDa single-pass type I membrane protein encoded by the c-fms gene that belongs to the type III protein tyrosine kinase receptor family. CD115 has an immunoglobulin-like extracellular domain, transmembrane domain, and C-terminal tail receptor and is expressed by monocytes, macrophages, plasmacytoid and conventional dendritic cells (DCs), osteoclasts, and their precursors. CD115 is the receptor for CSF1, also known as M-CSF, and IL-34. Binding and signaling through CD115 regulates the proliferation, differentiation, survival, and cytokine-production of monocytes and macrophages1,2. In addition, CD115 plays a key role in the differentiation and proliferation of osteoclasts as well as their bone resorption activity3. Mutations in CSF-1R are associated with cancer, including myeloid malignancies, Alzheimer’s disease, and other inflammatory and autoimmune diseases4. The presence of tumor-associated macrophages (TAMs) expressing CSF-1R correlates with poor survival in various tumor types5,6, and efforts to eliminate these TAMs using CSF1R small-molecule inhibitors and monoclonal antibodies are currently in clinical trials7.
| Technical Datasheet: | View |
| Protein: | CD115 |
| Ligand/Receptor: | Macrophage colony stimulating factor (M-CSF), IL-34 |
| Research Area: | Immunology |
| Rat IgG2a Isotype Control | |
|---|---|
| Clone | 1-1 |
| Isotype | Rat IgG2a |
| Endotoxin Level | Ultra Low Endotoxin |
