Anti-Mouse CD279 (PD-1) - Fc Reduced (LALA-PG) Recombinant Monoclonal Antibody
Introducing the Anti-Mouse CD279 (PD-1) Fc Reduced LALA-PG Recombinant Monoclonal Antibody from Assay Genie, an exceptional tool for researchers focusing on immune regulation and checkpoint pathways. This antibody targets Programmed Cell Death Protein 1 (PD-1), a critical checkpoint protein involved in the regulation of T-cell function and immune response. Engineered with a unique LALA-PG modification, this antibody ensures reduced Fc receptor binding, enhancing specificity and minimizing off-target effects. Ideal for applications in immunology research, PD-1 blockade studies, and cancer immunotherapy research, it offers unparalleled performance in various assays such as ELISA, flow cytometry, and functional assays. This product boasts high purity and low endotoxin levels, ensuring reliable and reproducible results.
It is available in a range of quantities to suit different experimental needs and is provided in a stable phosphate-buffered saline solution. Enhance your research capabilities with this versatile and high-quality antibody, designed to deliver precise and impactful results in studies related to immune checkpoints and therapeutic targets. PD-1, a crucial immune regulatory molecule, is predominantly expressed on T cells and plays a significant role in maintaining immune tolerance by inhibiting T-cell activation upon engagement with its ligands, PD-L1 or PD-L2. Understanding and targeting PD-1 pathways can lead to significant advancements in treatments for cancer, autoimmune diseases, and chronic infections.
Product Name:
Anti-Mouse CD279 (PD-1) - Fc Reduced (LALA-PG) Recombinant Monoclonal Antibody
Product Code:
IVMB0280
Clone:
RMP1-14 (LALA-PG)
Protein:
PD-1
Product Type:
Recombinant Monoclonal Antibody
Synonyms:
Programmed Death-1, CD279, PD1, PDCD1
Isotype:
Mouse IgG2a k
Reactivity:
Mouse
Applications:
B, FA, In Vivo, WB
Formulation:
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<1.0 EU/µg
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
B, FA, In Vivo, WB
Reactivity:
Mouse
Host Species:
Mouse-Rat Chimeric
Specificity:
Anti-PD1 antibody (clone RMP1-14) recognizes an epitope on mouse PD-1.
Antigen Distribution:
PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells.
Immunogen:
Mouse PD-1 transfected BHK cells
Concentration:
≤1.0 EU/mg
Endotoxin Level:
<1.0 EU/µg
Formulation:
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥95% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
This anti-PD1 antibody is a recombinant chimeric version of the original clone RMP1-14 antibody. The variable domain sequences are identical to the original clone RMP1-14; however, the constant region sequences have been switched from rat IgG2a to mouse IgG2a. In this Fc Mutedâ„¢ format, mutations (LALA-PG) have been introduced at crucial binding sites of the Fc domain with the FcR and C1q, reducing or eliminating Fc-mediated Antibody Dependent Cellular Phagocytosis (ADCP).
PD-1 is a 50-55 kD member of the B7 Ig superfamily. PD-1 is also a member of the extended CD28/CTLA-4 family of T cell regulators and is suspected to play a role in lymphocyte clonal selection and peripheral tolerance. The ligands of PD-1 are PD-L1 and PD-L2, and are also members of the B7 Ig superfamily. PD-1 and its ligands negatively regulate immune responses. PD-L1, or B7-Homolog 1, is a 40 kD type I transmembrane protein that has been reported to costimulate T cell growth and cytokine production. The interaction of PD-1 with its ligand PD-L1 is critical in the inhibition of T cell responses that include T cell proliferation and cytokine production. PD-L1 has increased expression in several cancers. Inhibition of the interaction between PD-1 and PD-L1 can serve as an immune checkpoint blockade by improving T-cell responses In vitro and mediating preclinical antitumor activity. Within the field of checkpoint inhibition, combination therapy using anti-PD1 in conjunction with anti-CTLA4 has significant therapeutic potential for tumor treatments. PD-L2 is a 25 kD type I transmembrane ligand of PD-1. Via PD-1, PD-L2 can serve as a co-inhibitor of T cell functions. Regulation of T cell responses, including enhanced T cell proliferation and cytokine production, can result from mAbs that block the PD-L2 and PD-1 interaction.
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's recombinant antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
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