![Anti-Mouse/Human CD45R (B220) [RA3-6B2] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/214515/569358/anti-mousehuman-cd45r-b220-ra3-6b2-in-vivo-antibody-low-endotoxin__87251.1770916167.jpg?c=2?imbypass=on "Anti-Mouse/Human CD45R (B220) [RA3-6B2] In Vivo Antibody - Low Endotoxin")
Description
Anti-Mouse/Human CD45R (B220) [RA3-6B2] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse/Human CD45R/B220 [RA3-6B2] In Vivo Antibody - Low Endotoxin from Assay Genie, a precise and highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD45R/B220 protein, a marker crucial in the identification and analysis of B cells, making it indispensable for research in immunology and related fields. With a rat IgG2a isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), making it perfectly suitable for ELISA, flow cytometry, immunohistochemistry, and various other assays.
Available in different sizes, this antibody is formulated in phosphate-buffered saline to guarantee stability and efficacy. Enhance your research with this trustworthy and versatile antibody. CD45R/B220 is a protein tyrosine phosphatase that plays a significant role in the regulation of B cell function. CD45R/B220 is predominantly expressed on B cells, serving as an important marker for B cell lineage and developmental stages. It is extensively used in studies involving B cell development, activation, and immune responses."
| Product Name: | Anti-Mouse/Human CD45R (B220) [RA3-6B2] In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0180 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | RA3-6B2 |
| Protein: | CD45R |
| Product Type: | Monoclonal Antibody |
| Synonyms: | B220 |
| Isotype: | Rat IgG2a κ |
| Reactivity: | Mouse/Human |
| Immunogen: | Abelson murine leukemia virus-induced pre-B tumor cells |
| Applications: | Act, CyTOF®, Depletion, FA, FC, IHC FF, IP, PhenoCycler® |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | Act, CODEX®, CyTOF®, Depletion, FA, FC, IHC FF, IP |
| Recommended Usage: | FC The suggested concentration for this RA3-6B2 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse/Human |
| Host Species: | Rat |
| Specificity: | Clone RA3-6B2 recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule. |
| Antigen Distribution: | The CD45R antigen is present on mouse B-cells, B-cell precursors and lytically active subsets of lymphokine-activated killer cells (NK cells and non-MHC restricted CTL). |
| Immunogen: | Abelson murine leukemia virus-induced pre-B tumor cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform.
| Technical Datasheet: | View |
| Protein: | CD45R |
| Function: | Phosphatase, T and B cell activation |
| Ligand/Receptor: | Galectin-1, CD2, CD3, CD4 |
| Research Area: | Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers |
![Anti-Mouse/Human CD45R (B220) [RA3-6B2] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/214515/569358/anti-mousehuman-cd45r-b220-ra3-6b2-in-vivo-antibody-low-endotoxin__87251.1770916167.jpg?c=2 "Anti-Mouse/Human CD45R (B220) [RA3-6B2] In Vivo Antibody - Low Endotoxin")
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![Anti-Mouse/Human CD44 [IM7] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214413/569499/anti-mousehuman-cd44-im7-in-vivo-antibody-low-endotoxin__61349.1770911829.jpg?c=2 "Anti-Mouse/Human CD44 [IM7] In Vivo Antibody - Low Endotoxin")
